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\u3000MA104\u7d30\u80de\u306f1994\u5e744\u6708\u306b\u3001\u82f1\u56fdEuropean Collection of Cell Culture \uff08ECACC\uff09\u304b\u3089\u3001\u30a2\u30ab\u30b2\u30b6\u30eb\u7531\u6765\u7d30\u80de\u682a\u3068\u3057\u3066\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u3067\u3059\u3002\u5bc4\u8a17\u5f53\u6642\u306f\u3001\u7d30\u80de\u682a\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u691c\u67fb\u65b9\u6cd5\u3068\u3057\u3066\u3001\uff12\u7a2e\u985e\u306e\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\uff08lactate dehydrogenase\u53ca\u3073nucleotide phosphorylase\uff09\u3092\u89e3\u6790\u3059\u308b\u691c\u67fb\u65b9\u6cd5\u304c\u4e00\u822c\u7684\u3067\u3042\u308a\u3001\u5f53\u30d0\u30f3\u30af\u3092\u542b\u3081\u3001ECACC\u3001\u7c73\u56fdATCC\u3001\u30c9\u30a4\u30c4DSMZ\u7b49\u306e\u4e16\u754c\u306e\u4e3b\u8981\u7d30\u80de\u30d0\u30f3\u30af\u304c\u5f53\u8a72\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3057\u305f\u3002MA104\u7d30\u80de\u306b\u3064\u304d\u307e\u3057\u3066\u3082\u3001\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3001ECACC\u304b\u3089\u306e\u60c5\u5831\u901a\u308a\u30a2\u30ab\u30b2\u30b6\u30eb\u7531\u6765\u3067\u3042\u308b\u3068\u5224\u5b9a\u3057\u3066\u63d0\u4f9b\u3092\u3057\u3066\u307e\u3044\u308a\u307e\u3057\u305f\u3002\u5c1a\u3001\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u3068\u306f\u3001\u591a\u7a2e\u985e\u306e\u52d5\u7269\u304c\u5171\u901a\u3057\u3066\u6301\u3063\u3066\u3044\u308b\u540c\u3058\u9175\u7d20\u3067\u3082\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u69cb\u9020\u304c\u7570\u306a\u308a\u3001\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u3063\u3066\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u3066\u3001\u52d5\u7269\u7a2e\u3092\u5224\u5b9a\u3059\u308b\u751f\u5316\u5b66\u7684\u306a\u691c\u67fb\u65b9\u6cd5\u3067\u3059\u3002
\n\u30002007\u5e74\u3001\u7d30\u80de\u304c\u7531\u6765\u3057\u305f\u52d5\u7269\u7a2e\u306e\u3088\u308a\u7cbe\u5ea6\u304c\u9ad8\u3044\u691c\u67fb\u6cd5\u3068\u3057\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u3092\u5bfe\u8c61\u3068\u3057\u305f\u5206\u5b50\u751f\u7269\u5b66\u7684\u306a\u7a2e\u306e\u540c\u5b9a\u6cd5\uff08\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\uff09\u304c\u958b\u767a\u3055\u308c\u307e\u3057\u305f\uff08\u6587\u732e\uff11\uff09\u3002\u3053\u306e\u691c\u67fb\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u306e\u5869\u57fa\u914d\u5217\u304c\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3059\u308b\u3082\u306e\u3067\u3042\u308a\u3001\u52d5\u7269\u7a2e\u7279\u7570\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3057\u3066\u3001PCR\u7523\u7269\u306e\u6709\u7121\u3067\u52d5\u7269\u7a2e\u3092\u691c\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u5f53\u5ba4\u3067\u3082\u30012011\u5e74\u3088\u308a\u3001\u65b0\u898f\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u30012011\u5e74\u4ee5\u524d\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u3001\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u7d50\u679c\u3092\u4fe1\u7528\u3057\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002
\n\u3000\u6628\u5e74\u30012011\u5e74\u4ee5\u524d\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u306e\u4e2d\u306b\u7531\u6765\u52d5\u7269\u7a2e\u304c\u5bc4\u8a17\u8005\u767b\u9332\u60c5\u5831\u3068\u7570\u306a\u308b\u7d30\u80de\u682a\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u767a\u898b\u3055\u308c\u305f\u305f\u3081\u30012011\u5e74\u4ee5\u524d\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u306b\u3064\u3044\u3066\u3082\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3068\u3044\u305f\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u691c\u67fb\u306e\u4e2d\u3067\u3001\u4eca\u56de\u306e\u4e8b\u5b9f\u304c\u5224\u660e\u3044\u305f\u3057\u307e\u3057\u305f\u3002
\n\u56f3\uff11\u306b\u793a\u3057\u307e\u3057\u305f\u3088\u3046\u306b\u3001MA104\u7d30\u80de\u3092\u5bfe\u8c61\u3068\u3057\u3066PCR\u3092\u5b9f\u65bd\u3057\u305f\u3068\u3053\u308d\u3001\u30a2\u30ab\u30b2\u30b6\u30eb\u3092\u540c\u5b9a\u3067\u304d\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u306fPCR\u7523\u7269\u304c\u5f97\u3089\u308c\u305a\u3001\u30a2\u30d5\u30ea\u30ab\u30df\u30c9\u30ea\u30b6\u30eb\u3092\u540c\u5b9a\u3067\u304d\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3066PCR\u7523\u7269\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002
\n\u3000\u5c1a\u3001ECACC\u306e\u73fe\u5728\u306e\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u3067\u306f\u300cMA104\u7d30\u80de\u306f\u3001\u6a39\u7acb\u8005\u304b\u3089\u306e\u767b\u9332\u60c5\u5831\u3067\u3042\u308b\u30a2\u30ab\u30b2\u30b6\u30eb\u7531\u6765\u3067\u306f\u306a\u304f\u3001\u30a2\u30d5\u30ea\u30ab\u30df\u30c9\u30ea\u30b6\u30eb\u7531\u6765\u3067\u3042\u308b\u300d\u3053\u3068\u304c\u8a18\u8f09\u3055\u308c\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u3000MA104\u7d30\u80de\u3092\u63d0\u4f9b\u3057\u305f\u5168\u3066\u306e\u5229\u7528\u8005\u306b\u306f\u3001\u9673\u8b1d\u3068\u8aac\u660e\u306e\u3054\u9023\u7d61\u3092\u5dee\u3057\u4e0a\u3052\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u3010\u4eca\u5f8c\u306e\u5bfe\u5fdc\u306b\u3064\u304d\u307e\u3057\u3066\u3011
\n\u30002011\u5e74\u4ee5\u524d\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u30d2\u30c8\u53ca\u3073\u30de\u30a6\u30b9\u4ee5\u5916\u306e\u52d5\u7269\u7531\u6765\u306e\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u304c\u53ef\u80fd\u306a\u52d5\u7269\u7a2e\u306b\u3064\u3044\u3066\u306f\u9806\u6b21\u691c\u67fb\u3092\u884c\u3063\u3066\u304a\u308a\u307e\u3059\u3002\u672a\u691c\u67fb\u306e\u7d30\u80de\u306e\u63d0\u4f9b\u4f9d\u983c\u304c\u3042\u3063\u305f\u5834\u5408\u306f\u3001\u63d0\u4f9b\u524d\u306b\u5fc5\u305a\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3068\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u3000\u5f15\u304d\u7d9a\u304d\u3001\u7406\u7814\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc\u306e\u4e8b\u696d\u306b\u5bfe\u3057\u3066\u3054\u7406\u89e3\u3068\u3054\u652f\u63f4\u3092\u8cdc\u308a\u305f\u304f\u3001\u5b9c\u3057\u304f\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<\/p>\n

\u3010\u6587\u732e\u3011<\/p>\n

    \n
  1. Ono, K. et al. Species identification of animal cells by nested PCR targeted to mitochondrial DNA. In Vitro Cell Dev Biol Anim. 43: 168-175 (2007)<\/li>\n<\/ol>\n

    \"\u56f3\uff11\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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