{"id":9607,"date":"2016-06-24T09:52:22","date_gmt":"2016-06-24T00:52:22","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/ja\/?page_id=9607"},"modified":"2021-12-24T16:15:56","modified_gmt":"2021-12-24T07:15:56","slug":"frozen","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/manual\/frozen","title":{"rendered":"\u7d30\u80de\u306e\u51cd\u7d50\u6cd5(\u30ac\u30e9\u30b9\u5316\u6cd5\uff1aVitrification\uff09(HPS\u30fbHES)"},"content":{"rendered":"<blockquote>\n<ul>\n<li>\u5f53\u5ba4\u304b\u3089\u306e\u9001\u4ed8\u306f\u901a\u5e38\u306e\u7d30\u80de\u8f38\u9001\u7528\u306e\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u4e2d\uff08\u7d04-80\u2103\uff09\u3067\u306f\u306a\u304f\u3001\u6db2\u4f53\u7a92\u7d20\u3092\u5438\u7740\u3055\u305b\u305f\u4fdd\u51b7\u5264\u306b\u3088\u308a\u7d04-170\u2103\u4ee5\u4e0b\u306b\u51b7\u5374\u3057\u305f\u30c9\u30e9\u30a4\u30b7\u30c3\u30d1\u30fc\u306b\u3066\u767a\u9001\u3057\u3066\u3044\u307e\u3059\u3002\n<\/ul>\n<p><strong>\u7d30\u80de\u6750\u6599\u304c\u5230\u7740\u3057\u307e\u3057\u305f\u3089\u3001\u76f4\u3061\u306b\u57f9\u990a\u3092\u958b\u59cb\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/strong><\/p>\n<p>\u203b\u3059\u3050\u306b\u57f9\u990a\u3092\u958b\u59cb\u3057\u306a\u3044\u5834\u5408\u306f<a href=\"\/ja\/distribution\/send_cell\">\u3053\u3061\u3089<\/a>\u3092\u53c2\u7167\u3057\u3066\u304f\u3060\u3055\u3044\u3002\n<\/blockquote>\n<p>\u203b\u767a\u9001\u6642\u306e\u6dfb\u4ed8\u66f8\u985e<a href=\"\/ja\/wp-content\/uploads\/quality\/caution.pdf\">\uff08PDF\uff09<\/a>\u306b\u3082HPS\u30fbHES\u306e\u518d\u57f9\u990a\u65b9\u6cd5\u306e\u8a18\u8f09\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><span style=\"color: #008000;\"><strong>\u306f\u3058\u3081\u306b<\/strong><\/span><\/p>\n<p>\u203b\u5f53\u5ba4\u3067\u306f<span style=\"color: #3344ff;\"><strong>\u300c\u30ac\u30e9\u30b9\u5316\u6cd5\uff08Vitrification\u6cd5\uff09\u300d<\/strong><\/span>\u3092\u7528\u3044\u3066\u30d2\u30c8iPS\u7d30\u80de\u304a\u3088\u3073\u30d2\u30c8ES\u7d30\u80de\uff08\u4ee5\u4e0b\u3001HPS\u30fbHES\uff09\u306e\u51cd\u7d50\u3092\u884c\u3063\u3066\u304a\u308a\u307e\u3059\u3002 \u7d30\u80de\u682a\u306e\u51cd\u7d50\u306b\u4e00\u822c\u7684\u306b\u826f\u304f\u7528\u3044\u3089\u308c\u3066\u3044\u308b\u3001DMSO\u3092\u7528\u3044\u30661\u2103\/1\u5206\u7a0b\u5ea6\u3067\u3086\u3063\u304f\u308a\u51b7\u5374\u3092\u884c\u3044\u3001\u7d30\u80de\u3092\u51cd\u7d50\u3059\u308b<span style=\"color: #3344ff;\"><strong>\u300c\u7de9\u6162\u51b7\u5374\u6cd5\u300d<\/strong><\/span>\u3068\u51cd\u7d50\u53ca\u3073\u878d\u89e3\u306e\u624b\u9806\u304c\u5927\u304d\u304f\u7570\u306a\u308a\u307e\u3059\u3002 \u672c\u9805\u3092\u3088\u304f\u8aad\u307f\u3001\u64cd\u4f5c\u624b\u9806\u3092\u3088\u304f\u7406\u89e3\u3055\u308c\u305f\u4e0a\u3067\u3001\u878d\u89e3\u4f5c\u696d\u3092\u958b\u59cb\u3059\u308b\u3053\u3068\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\uff08<a href=\"#ref\">\u53c2\u8003\u6587\u732e<\/a>\u53c2\u7167\uff09\u3002<\/p>\n<p><span style=\"color: #008000;\"><strong>\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044<\/strong><\/span><\/p>\n<ul>\n<li>HPS\u30fbHES\u306e\u51cd\u7d50\u4fdd\u5b58\u6db2\u306f\u6db2\u91cf\u304c\u5c11\u306a\u304f(200 \u03bcl) \u878d\u70b9\u304c\u4f4e\u3044\uff08-65\u2103\u524d\u5f8c\uff09\u305f\u3081\u3001\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u306e\u6e29\u5ea6\u304c<span style=\"color: #ff0000;\"><strong>-140\u2103\u4ee5\u4e0a\u306b\u4e0a\u304c\u3089\u306a\u3044\u3088\u3046\u6ce8\u610f<\/strong><\/span>\u3057\u3001\u4fdd\u7ba1\u5834\u6240\u304b\u3089\u4f5c\u696d\u7528\u306e\u30af\u30ea\u30fc\u30f3\u30d9\u30f3\u30c1\u306b\u904b\u3076\u969b\u306f\u3001\u305f\u3068\u3048\u8fd1\u8ddd\u96e2\u3067\u3082\u3001<span style=\"color: #ff0000;\"><strong>\u6db2\u4f53\u7a92\u7d20\u306b\u6d6e\u304b\u305b\u3066\u78ba\u5b9f\u306b\u51b7\u5374\u3057\u305f\u72b6\u614b\u3067\u904b\u3093\u3067\u304f\u3060\u3055\u3044<\/strong><\/span>\u3002\n<li>\u5f53\u5ba4\u304b\u3089\u9001\u4ed8\u3055\u308c\u305f\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u306f\u3001\u3054\u304f\u307e\u308c\u306b\u7834\u88c2\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u4fdd\u7ba1\u3084\u878d\u89e3\u306e\u969b\u306b\u306f<span style=\"color: #ff0000;\"><strong>\u624b\u888b\u3068\u30d5\u30a7\u30a4\u30b9\u30ac\u30fc\u30c9<\/strong><\/span>\u3092\u7740\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002\n<li>\u4f5c\u696d\u4e2d\u306e\u6e29\u5ea6\u5909\u5316\u306b\u3088\u308a\u3001\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u306e\u84cb\u304c\u7de9\u3080\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u84cb\u304c\u7de9\u3093\u3067\u3044\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3001\u5145\u5206\u6ce8\u610f\u3057\u3066\u4f5c\u696d\u3092\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002\n<li>\u30ac\u30e9\u30b9\u5316\u6cd5\u306f\u51cd\u7d50\u4fdd\u5b58\u6db2\u306e\u6bd2\u6027\u304c\u975e\u5e38\u306b\u9ad8\u304f\u3001\u7d30\u80de\u304c\u4f5c\u696d\u4e2d\u306b\u878d\u89e3\u3057\u305f\u9ad8\u6fc3\u5ea6\u306e\u51cd\u7d50\u4fdd\u5b58\u6db2\u306b\u89e6\u308c\u308b\u6642\u9593\u304c\u3067\u304d\u308b\u9650\u308a\u77ed\u304f\u306a\u308b\u3088\u3046\u306b\u6025\u901f\u306b\u878d\u89e3\u3057\u3001\u57f9\u5730\u3067\u5e0c\u91c8\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\n<li><span style=\"color: #ff0000;\"><strong>\u6db2\u4f53\u72b6\u614b\u306e\u4fdd\u5b58\u6db2\u306b\u89e6\u308c\u308b\u3068\u3001\u7d30\u80de\u306f1\u5206\u7a0b\u5ea6\u3067\u307b\u3068\u3093\u3069\u304c\u6b7b\u6ec5\u3057\u3066\u3057\u307e\u3044\u307e\u3059<\/strong><\/span>\u3002\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u3092\u6db2\u4f53\u7a92\u7d20\u304b\u3089\u53d6\u308a\u51fa\u3057\u3066\u304b\u3089\u3001\u57f9\u5730\u306b\u623b\u3059\u307e\u3067\u306e\u64cd\u4f5c\u3092\u76ee\u5b89\u3068\u3057\u306630\uff5e40\u79d2\u7a0b\u5ea6\u3067\u884c\u3063\u3066\u4e0b\u3055\u3044\u3002\n<\/ul>\n<hr \/>\n<p><a name=\"1\"><\/a><br \/>\n<span style=\"color: #808000;\"><strong>\u30d2\u30c8iPS \u7d30\u80de\u304a\u3088\u3073\u30d2\u30c8ES\u7d30\u80de(HPS\u30fbHES)\u306e\u878d\u89e3\u65b9\u6cd5<\/strong><\/span><\/p>\n<p><span style=\"color: #008000;\"><strong>\u6e96\u5099\u3059\u308b\u3082\u306e<\/strong><\/span><\/p>\n<ul>\n<li>HPS\u30fbHES\u306e\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\uff08\u30ac\u30e9\u30b9\u5316\u6cd5\u3067\u51cd\u7d50\u3057\u305f\u3082\u306e\uff09\n<li>\u30d5\u30a3\u30fc\u30c0\u30fc\u7d30\u80de\u3092\u64ad\u7a2e\u3057\u305f\u57f9\u990a\u30c7\u30a3\u30c3\u30b7\u30e5<br \/>\uff08\u878d\u89e3\u5f8c\u306eHPS\u30fbHES\u3092\u64ad\u7a2e\u3059\u308b\u305f\u3081\u3001\u5404\u7d30\u80de\u682a\u6307\u5b9a\u306e\u3082\u306e\u3092\u30c7\u30fc\u30bf\u30b7\u30fc\u30c8\u8a18\u8f09\u306e\u64ad\u7a2e\u5bc6\u5ea6\u306b\u5f93\u3063\u306660 mm dish 2\u679a\uff0b\u4e88\u50991\u679a\u3092\u6e96\u5099\u3057\u3066\u304a\u304f\uff09\n<li>HPS\u30fbHES\u306e\u57f9\u990a\u57f9\u5730 \uff08\u5404\u7d30\u80de\u682a\u306b\u5fdc\u3058\u305f\u6307\u5b9a\u306e\u57f9\u5730\uff09\n<li>\u6db2\u4f53\u7a92\u7d20\u3092\u5165\u308c\u305f\u5bb9\u5668\uff08\u51cd\u7d50\u7d30\u80de\u306e\u79fb\u52d5\u306b\u4f7f\u7528\uff09\n<li>\u30d4\u30f3\u30bb\u30c3\u30c8\uff08\u6db2\u4f53\u7a92\u7d20\u304b\u3089\u306e\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u53d6\u308a\u51fa\u3057\u306b\u4f7f\u7528\uff09\n<li>\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u7acb\u3066\n<li>15 ml\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\n<li>1000 \u03bcl\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u53ca\u3073\u30c1\u30c3\u30d7\n<li>\u305d\u306e\u4ed6\u3001\u57f9\u990a\u64cd\u4f5c\u306b\u5fc5\u8981\u306a\u5668\u5177\n<\/ul>\n<p><span style=\"color: #008000;\"><strong>\u64cd\u4f5c\u624b\u9806<\/strong><\/span><\/p>\n<ol class=\"list_parentheses\">\n<li>15 ml\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u306b\u57f9\u990a\u57f9\u5730\u309210 ml\u5165\u308c\u3001\u30d1\u30e9\u30d5\u30a3\u30eb\u30e0\u3092\u3057\u3066\u30a6\u30a9\u30fc\u30bf\u30fc\u30d0\u30b9\u306737\u2103\u306b\u6e29\u3081\u3066\u304a\u304f\u3002\n<li>\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u3092\u4fdd\u7ba1\u5834\u6240\u304b\u3089\u6db2\u4f53\u7a92\u7d20\u3092\u5165\u308c\u305f\u5bb9\u5668\u306b\u79fb\u3057\u3001\u51b7\u5374\u3057\u305f\u72b6\u614b\u3067\u30af\u30ea\u30fc\u30f3\u30d9\u30f3\u30c1\u307e\u3067\u904b\u3076\u3002\n<li><span style=\"color: #ff0000;\"><strong>\u878d\u89e3\u76f4\u524d\u307e\u306737\u2103\u306b\u6e29\u3081\u3066\u304a\u3044\u305f\u57f9\u5730<\/strong><\/span>\u3092\u30af\u30ea\u30fc\u30f3\u30d9\u30f3\u30c1\u5185\u306b\u5165\u308c\u3001\u878d\u89e3\u64cd\u4f5c\u3092\u958b\u59cb\u3059\u308b\u3002\n<li>\u30d4\u30f3\u30bb\u30c3\u30c8\u3067\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u3092\u6db2\u4f53\u7a92\u7d20\u304b\u3089\u53d6\u308a\u51fa\u3057\u3001<span style=\"color: #ff0000;\"><strong>\u5fc5\u305a\u6e29\u3081\u305f\u57f9\u57301 ml\u30921000\u03bcl\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3067\u7d30\u80de\u51cd\u7d50\u6db2\u306b\u5168\u91cf\u3092\u5439\u304d\u3064\u3051\u308b\u3088\u3046\u306b\u5927\u304d\u304f10\u56de\u7a0b\u5ea6\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3044<\/strong><\/span>\u3001\u5b8c\u5168\u306b\u878d\u89e3\u5f8c\u3001\u3059\u3050\u306b\u7d30\u80de\u61f8\u6fc1\u6db2\u3092(3)\u306e\u6e29\u3081\u305f\u57f9\u5730\u306b\u56de\u53ce\u3059\u308b\u3002\n<div style=\"border:3px solid #1e90ff; margin: 5px; padding: 5px;\">\n\u6ce8)\u3000\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u3092\u6db2\u4f53\u7a92\u7d20\u304b\u3089\u53d6\u308a\u51fa\u3057\u3066\u304b\u3089\u3001\u57f9\u5730\u306b\u623b\u3059\u307e\u3067\u306e\u64cd\u4f5c\u3092\u3067\u304d\u308b\u3060\u3051\u8fc5\u901f\u306b\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002<span style=\"color: #ff0000;\"><strong>\u76ee\u5b89\u306f30\uff5e40\u79d2\u7a0b\u5ea6<\/strong><\/span>\u3002<br \/>\n\u6ce8)\u3000\u3053\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3001\u51b7\u3081\u305f\u57f9\u5730\u306e\u4f7f\u7528\u3001\u4e0d\u5145\u5206\u306a\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u7b49\u306e\u8981\u56e0\u306b\u3088\u308a\u3001<span style=\"color: #ff0000;\"><strong>\u518d\u51cd\u7d50\u304c\u8d77\u304d\u308b\u3068\u751f\u5b58\u7387\u304c\u8457\u3057\u304f\u4f4e\u4e0b\u3057\u307e\u3059<\/strong><\/span>\u3002<\/p>\n<\/div>\n<li>200xG (1,000 rpm)\u3001\u5ba4\u6e29\u30013\u5206\u9593\u9060\u5fc3\u3057\u307e\u3059\u3002\n<li>\u4e0a\u6e05\u3092\u9664\u304d\u3001\u57f9\u990a\u57f9\u57304\uff5e5 ml\u306b\u61f8\u6fc1\u5f8c\u3001\u7528\u610f\u3057\u3066\u304a\u3044\u305f\u30d5\u30a3\u30fc\u30c0\u30fc\u7d30\u80de\u306e\u57f9\u990a\u30c7\u30a3\u30c3\u30b7\u30e5\u306b\u64ad\u7a2e\u3057\u3066\u57f9\u990a\u3092\u958b\u59cb\u3059\u308b\u3002<span style=\"color: #ff0000;\"><strong>(\u51cd\u7d50\u30c1\u30e5\u30fc\u30d61\u672c\u306b\u5bfe\u3057\u306660 mm dish 1\uff5e2\u679a)<\/strong><\/span>\n<li>\u57f9\u990a\u4e2d\u306f\u6bce\u65e5\u57f9\u5730\u4ea4\u63db\u3068\u89b3\u5bdf\u3092\u884c\u3046\u3002\n<div style=\"border:3px solid #1e90ff; margin: 5px; padding: 5px;\">\n\u6ce8\uff09\u3000\u878d\u89e3\u7fcc\u65e5\u306b\u7d30\u80de\u584a\u304c\u6d6e\u904a\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u304c\u300160mm dish\u5168\u4f53\u3067\u6570\u5341\u500b\u7a0b\u5ea6\u306e\u30b3\u30ed\u30cb\u30fc\u306e\u63a5\u7740\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u63a5\u7740\u3057\u3066\u3044\u308b\u30b3\u30ed\u30cb\u30fc\u6570\u304c\u5c11\u306a\u304f\u3001\u6d6e\u904a\u3057\u3066\u3044\u308bHPS\u30fbHES\u306e\u7d30\u80de\u584a\u304c\u591a\u3044\u5834\u5408\u306f\u3001\u4e0a\u6e05\u3092\u9060\u5fc3\u3057\u3066\u53d6\u308a\u9664\u304d\u3001\u7d30\u80de\u3092\u65b0\u3057\u3044\u57f9\u5730\u306b\u61f8\u6fc1\u5f8c\u3001\u5143\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u306b\u64ad\u304d\u76f4\u3057\u3066\u304f\u3060\u3055\u3044\u3002\n<\/div>\n<li>\u30c7\u30a3\u30c3\u30b7\u30e5\u5168\u4f53\u306e7\uff5e8\u5272\u306e\u30b3\u30ed\u30cb\u30fc\u304c\u5927\u304d\u304f\u306a\u308a\u3001\u5206\u5316\u3092\u59cb\u3081\u308b\u524d\u306b\u7d99\u4ee3\u64cd\u4f5c\u3092\u884c\u3044\u307e\u3059\u3002\u7d99\u4ee3\u6642\u306b\u4f7f\u7528\u3059\u308b\u30d5\u30a3\u30fc\u30c0\u30fc\u7d30\u80de\u3092\u5fd8\u308c\u306a\u3044\u3088\u3046\u306b\u6e96\u5099\u3057\u3066\u304a\u304f\u3002\n<\/ol>\n<p>\u53c2\u8003\u6587\u732e<br 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