{"id":9235,"date":"2016-01-26T20:35:13","date_gmt":"2016-01-26T11:35:13","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/ja\/?page_id=9235"},"modified":"2016-06-15T15:48:30","modified_gmt":"2016-06-15T06:48:30","slug":"myo","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/quality\/myo","title":{"rendered":"\u7b4b\u7d30\u80de\u3078\u306e\u5206\u5316\u8a98\u5c0e\u691c\u67fb"},"content":{"rendered":"<p>C2C12\u7d30\u80de\u682a\u306e\u7279\u6027\u3067\u3042\u308b\u7b4b\u7d30\u80de\u3078\u306e\u5206\u5316\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<br \/>\n<a href=\"http:\/\/cell.brc.riken.jp\/ja\/wp-content\/uploads\/2016\/03\/4DG\u5206\u5316\u8a98\u5c0e\u7b4bC2C12\u5f62\u614b\u5199\u771f-e1457406866354.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/2016\/03\/4DG\u5206\u5316\u8a98\u5c0e\u7b4bC2C12\u5f62\u614b\u5199\u771f-e1457406866354.jpg\" alt=\"4DG\u5206\u5316\u8a98\u5c0e(\u7b4b)C2C12\u5f62\u614b\u5199\u771f\" width=\"300\" height=\"225\" class=\"alignright size-full wp-image-9346\" \/><\/a><br \/>\n<b>1. <\/b><b>\u6e96\u5099\u3059\u308b\u3082\u306e<\/b><br \/>\n\u30fb\u691c\u67fb\u5bfe\u8c61\uff1aRCB0987 C2C12\u7d30\u80de\uff08\u878d\u89e3\u5f8c\u30011\u9031\u9593\u7a0b\u5ea6\u57f9\u990a\u3057\u305f\u7d30\u80de\u3092\u4f7f\u7528\uff09<br \/>\n\u30fb\u57f9\u990a\u57f9\u5730\uff1aDMEM\uff0b10% FBS<br \/>\n\u30fb\u5206\u5316\u8a98\u5c0e\u57f9\u5730\uff1aDMEM\uff0b2% HS<br \/>\n\u30fb\u57f9\u990a\u5668\u5177\u4e00\u5f0f<\/p>\n<p><b>2. <\/b><b>\u65b9\u6cd5<\/b><br \/>\n(1)\u3000\u901a\u5e38\u306e\u57f9\u990a\u57f9\u5730\u3092\u4f7f\u7528\u3057\u3001\u03c660mmdish 1\u679a\u306b\u7d30\u80de\u3092\u64ad\u7a2e\u3059\u308b\u3002<br \/>\n(2)\u300060\uff5e80% \u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u307e\u3067\u5897\u6b96\u3057\u305f\u3089\u3001\u5206\u5316\u8a98\u5c0e\u57f9\u5730\u30671\uff5e2\u65e5\u6bce\u306b\u57f9\u5730\u4ea4\u63db\u3092\u884c\u3046\u3002<br \/>\n(3)\u3000\u5206\u5316\u8a98\u5c0e\u57f9\u5730\u3067\u57f9\u5730\u4ea4\u63db\u958b\u59cb\u5f8c\u30015\u65e5\u7a0b\u3067\u5206\u5316\u3057\u305f\u7d30\u80de\u304c\u89b3\u5bdf\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\u3002<br \/>\n\u3000\u3000\u3000\u203b\u7d041\uff5e2\u9031\u9593\u7a0b\u5ea6\u3001\u57f9\u5730\u4ea4\u63db\u3092\u884c\u3044\u3001\u89b3\u5bdf\u3059\u308b\u3002<br \/>\n(4)\u3000\u5f62\u614b\u5199\u771f\u3092\u64ae\u5f71\u3057\u3001\u8a18\u9332\u3059\u308b\u3002<\/p>\n<p><b>3. <\/b><b>\u7d50\u679c\u306e\u5224\u5b9a<\/b><br \/>\n\u5206\u5316\u8a98\u5c0e\u57f9\u5730\u3067\u57f9\u5730\u4ea4\u63db\u3092\u884c\u3046\u524d\u306e\u5f62\u614b\u3068\u6bd4\u8f03\u3057\u3001\u7b4b\u7d30\u80de\u304c\u51fa\u73fe\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u3002<\/p>\n<p>\u203b\u5206\u5316\u8a98\u5c0e\u57f9\u5730\u3067\u57f9\u5730\u4ea4\u63db\u958b\u59cb\u5f8c<\/p>\n<p><a href=\"http:\/\/cell.brc.riken.jp\/ja\/wp-content\/uploads\/2016\/01\/RCB0987-C2C12-.jpg\"><img loading=\"lazy\" decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/2016\/01\/RCB0987-C2C12-.jpg\" alt=\"RCB0987 C2C12\" width=\"1445\" height=\"345\" class=\"alignleft size-full wp-image-9955\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>C2C12\u7d30\u80de\u682a\u306e\u7279\u6027\u3067\u3042\u308b\u7b4b\u7d30\u80de\u3078\u306e\u5206\u5316\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002 1. \u6e96\u5099\u3059\u308b\u3082\u306e \u30fb\u691c\u67fb\u5bfe\u8c61\uff1aRCB0987 C2C12\u7d30\u80de\uff08\u878d\u89e3\u5f8c\u30011\u9031\u9593\u7a0b\u5ea6\u57f9\u990a\u3057\u305f\u7d30\u80de\u3092\u4f7f\u7528\uff09 \u30fb\u57f9\u990a\u57f9\u5730\uff1aDMEM\uff0b10% FBS \u30fb\u5206\u5316\u8a98\u5c0e\u57f9\u5730\uff1aDMEM\uff0b2% HS \u30fb\u57f9\u990a\u5668\u5177\u4e00\u5f0f 2. \u65b9\u6cd5 (1)\u3000\u901a\u5e38\u306e\u57f9\u990a\u57f9\u5730\u3092\u4f7f\u7528\u3057\u3001\u03c660mmdish 1\u679a\u306b\u7d30\u80de\u3092\u64ad\u7a2e\u3059\u308b\u3002 (2)\u300060\uff5e80% \u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u307e\u3067\u5897\u6b96 [&hellip;]<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":3425,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-9235","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/9235","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=9235"}],"version-history":[{"count":8,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/9235\/revisions"}],"predecessor-version":[{"id":9568,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/9235\/revisions\/9568"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3425"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=9235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}