{"id":8317,"date":"2015-03-11T18:45:20","date_gmt":"2015-03-11T09:45:20","guid":{"rendered":"http:\/\/cell0.cell.rbrc.jp\/?page_id=8317"},"modified":"2026-04-20T15:18:20","modified_gmt":"2026-04-20T06:18:20","slug":"23cln","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/announcement\/23cln","title":{"rendered":"23CLN\u7d30\u80de\u306b\u3064\u3044\u3066\u306e\u304a\u77e5\u3089\u305b"},"content":{"rendered":"

\u3000\u30a6\u30b7\u7531\u6765\u7d30\u80de\u682a\u3068\u3057\u3066\u63d0\u4f9b\u3057\u3066\u304d\u307e\u3057\u305f23CLN\u7d30\u80de\uff08\u7406\u7814\u767b\u9332\u756a\u53f7RCB0179\uff09\u304c\u30a6\u30b7\u7531\u6765\u7d30\u80de\u682a\u3067\u306f\u306a\u3044\u3053\u3068\u304c\u5224\u660e\u3044\u305f\u3057\u307e\u3057\u305f\u3002\u3054\u5831\u544a\u3092\u7533\u3057\u4e0a\u3052\u307e\u3059\u3068\u3068\u3082\u306b\u3001\u5229\u7528\u8005\u306e\u7686\u69d8\u306b\u6df1\u304f\u304a\u8a6b\u3073\u7533\u3057\u4e0a\u3052\u308b\u6b21\u7b2c\u3067\u3059\u3002<\/p>\n

\u300023CLN\u7d30\u80de\u306f1987\u5e746\u6708\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u3067\u3059\u3002\u5bc4\u8a17\u5f53\u6642\u306b\u3001\u7d30\u80de\u682a\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u691c\u67fb\u3059\u308b\u4e00\u822c\u7684\u306a\u65b9\u6cd5\u3068\u3057\u3066\u4ed6\u306e\u7d30\u80de\u30d0\u30f3\u30af\uff08\u7c73\u56fdATCC\u7b49\uff09\u3067\u3082\u5b9f\u65bd\u3057\u3066\u3044\u305f\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\uff08\u540c\u4f4d\u9175\u7d20\uff09\u691c\u67fb\u3092\u3001\uff12\u7a2e\u985e\u306e\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0lactate dehydrogenase\u53ca\u3073nucleotide phosphorylase\u3092\u5bfe\u8c61\u3068\u3057\u3066\u5b9f\u65bd\u3057\u3001\u5bc4\u8a17\u8005\u306e\u60c5\u5831\u901a\u308a\u30a6\u30b7\u7531\u6765\u3067\u3042\u308b\u3068\u5224\u5b9a\u3057\u3066\u63d0\u4f9b\u3092\u3057\u3066\u307e\u3044\u308a\u307e\u3057\u305f\u3002\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u3068\u306f\u3001\u591a\u7a2e\u985e\u306e\u52d5\u7269\u304c\u5171\u901a\u3057\u3066\u6301\u3063\u3066\u3044\u308b\u540c\u3058\u9175\u7d20\u3067\u3082\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u69cb\u9020\u304c\u7570\u306a\u308a\u3001\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u3063\u3066\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u3066\u3001\u52d5\u7269\u7a2e\u3092\u5224\u5b9a\u3059\u308b\u751f\u5316\u5b66\u7684\u306a\u691c\u67fb\u65b9\u6cd5\u3067\u3059\u3002
\n\u3000\u8fd1\u5e74\u3001\u7d30\u80de\u304c\u7531\u6765\u3057\u305f\u52d5\u7269\u7a2e\u306e\u3088\u308a\u7cbe\u5ea6\u304c\u9ad8\u3044\u691c\u67fb\u6cd5\u3068\u3057\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u3092\u5bfe\u8c61\u3068\u3057\u305f\u5206\u5b50\u751f\u7269\u5b66\u7684\u306a\u7a2e\u306e\u540c\u5b9a\u6cd5\uff08\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\uff09\u304c\u958b\u767a\u3055\u308c\u307e\u3057\u305f\uff08\u6587\u732e\uff11\uff09\u3002\u5f53\u5ba4\u3067\u3082\u30012011\u5e74\u3088\u308a\u3001\u65b0\u898f\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u30012011\u5e74\u4ee5\u524d\u306b\u5bc4\u8a17\u3055\u308c\u305f\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u3001\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u7d50\u679c\u3067\u53ef\u3068\u3057\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002
\n\u3000\u5148\u65e5\u300123CLN\u7d30\u80de\u306e\u5229\u7528\u8005\u304b\u3089\u300c23CLN\u7d30\u80de\u3092\u7528\u3044\u3066\u30a6\u30b7\u306e\u907a\u4f1d\u5b50\u306e\u5358\u96e2\u3092\u8a66\u307f\u305f\u304c\u3001PCR\u306b\u3066\u5358\u96e2\u3067\u304d\u306a\u3044\u300d\u3068\u306e\u554f\u3044\u5408\u305b\u3092\u53d7\u3051\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3068\u3053\u308d\u300123CLN\u7d30\u80de\u306f\u30a6\u30b7\u7531\u6765\u3067\u306f\u306a\u304f\u30d6\u30bf\u7531\u6765\u306e\u7d30\u80de\u3067\u3042\u308b\u3068\u5224\u5b9a\u3055\u308c\u307e\u3057\u305f\u3002\u5bc4\u8a17\u76f4\u5f8c\u306e\u51cd\u7d50\u4fdd\u5b58\u7d30\u80de\u3082\u540c\u3058\u691c\u67fb\u7d50\u679c\u3067\u3042\u308a\u3001\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u6642\u70b9\u304b\u3089\u30d6\u30bf\u7531\u6765\u306e\u7d30\u80de\u3067\u3042\u3063\u305f\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u3000\u5c1a\u300123CLN\u7d30\u80de\u3092\u63d0\u4f9b\u3057\u305f\u5168\u3066\u306e\u5229\u7528\u8005\u306b\u306f\u30012015\u5e743\u67084\u65e5\u307e\u3067\u306b\u9673\u8b1d\u3068\u8aac\u660e\u306e\u3054\u9023\u7d61\u3092\u5dee\u3057\u4e0a\u3052\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u3010\u4eca\u5f8c\u306e\u5bfe\u5fdc\u306b\u3064\u3044\u3066\u3011
\n\u3000\u4eca\u56de\u306e\u4e8b\u4f8b\u3092\u8e0f\u307e\u3048\u3066\u3001\u30d2\u30c8\u53ca\u3073\u30de\u30a6\u30b9\u4ee5\u5916\u306e\u52d5\u7269\u7531\u6765\u306e\u7d30\u80de\u682a\u306b\u3064\u3044\u3066\u306f\u30012011\u5e74\u4ee5\u524d\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u3082\u542b\u3081\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2DNA\u540c\u5b9a\u691c\u67fb\u306b\u3088\u308b\u691c\u67fb\u304c\u53ef\u80fd\u306a\u52d5\u7269\u7a2e\u306b\u3064\u3044\u3066\u306f\u3001\u63d0\u4f9b\u524d\u306b\u691c\u67fb\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3068\u3044\u305f\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u3000\u5c1a\u3001\u5f53\u5ba4\u3067\u306f\u3001\u30d2\u30c8\u7d30\u80de\u682a\u306e\u30eb\u30fc\u30c1\u30f3\u691c\u67fb\u3068\u3057\u3066\u30012004\u5e74\u304b\u3089STR\uff08Short tandem repeat\uff09\u591a\u578b\u89e3\u6790\u3092\u5c0e\u5165\u3057\u3001\u8aa4\u8a8d\u30d2\u30c8\u7d30\u80de\u682a\u306e\u6392\u9664\u306b\u52aa\u3081\u3066\u304a\u308a\u307e\u3059\uff08\u6587\u732e\uff12\uff09\u3002\u307e\u305f\u3001\u30de\u30a6\u30b9\u7d30\u80de\u682a\u306b\u95a2\u3057\u307e\u3057\u3066\u306f\u3001\u7531\u6765\u3057\u305f\u30de\u30a6\u30b9\u7cfb\u7d71\u3092\u540c\u5b9a\u3059\u308bSSLP (Simple Sequence Length Polymorphism)\u89e3\u6790\u3092\u5c0e\u5165\u3057\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u3059\uff08\u6587\u732e\uff13\uff09\u3002<\/p>\n

\u3000\u672c\u4ef6\u306b\u95a2\u3059\u308b\u3054\u8cea\u554f\u3084\u3054\u8981\u671b\u7b49\u304c\u3054\u3056\u3044\u307e\u3057\u305f\u3089\u3001\u3054\u9060\u616e\u306a\u304f\u4e0b\u8a18\u307e\u3067\u3054\u9023\u7d61\u3044\u305f\u3060\u304d\u307e\u3059\u3088\u3046\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n

\u3000\u5f15\u304d\u7d9a\u304d\u3001\u7406\u7814\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc\u306e\u4e8b\u696d\u306b\u5bfe\u3057\u3066\u3054\u7406\u89e3\u3068\u3054\u652f\u63f4\u3092\u8cdc\u308a\u305f\u304f\u3001\u5b9c\u3057\u304f\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<\/p>\n

\u3010\u6587\u732e\u3011<\/p>\n

    \n
  1. Ono, K. et al. Species identification of animal cells by nested PCR targeted to mitochondrial DNA. In Vitro Cell Dev Biol Anim. 43: 168-175 (2007)<\/li>\n
  2. Yoshino, K., et al. Essential role for gene profiling analysis in the authentication of human cell lines. Hum. Cell 19: 43-48 (2006)<\/li>\n
  3. Yoshino, K., et al. Development of a simple method to determine the mouse strain from which cultured cell lines originated. Interdisciplinary Bio Central 2: Article No. 14 (open access journal) doi: 10.4051\/ibc.2010.2.4.0014 (2010)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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