{"id":3715,"date":"2013-04-12T10:50:59","date_gmt":"2013-04-12T01:50:59","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3715"},"modified":"2017-07-12T12:02:44","modified_gmt":"2017-07-12T03:02:44","slug":"confirm_report","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/quality\/origin\/confirm_report","title":{"rendered":"\u30d2\u30c8\u7531\u6765\u7d30\u80de\u682a\u306e\u8d77\u6e90\u306e\u78ba\u8a8d\u306b\u95a2\u3059\u308b  Short Tandem Repeat-Polymerase Chain Reaction\u6cd5\u306b\u3088\u308b\u89e3\u6790\u7d50\u679c\u306b\u3064\u3044\u3066"},"content":{"rendered":"<p style=\"text-align: right; padding-left: 30px;\"><a href=\"\/ja\/quality\/str\/origin\">\u623b\u308b<\/a><a href=\"\/ja\/quality\/str\/origin\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_back.png\" width=\"16\" height=\"16\" border=\"0\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<table width=\"100%\">\n<tbody>\n<tr>\n<td style=\"background-color:#ffffff;border:0px;width: 20%;\"><\/td>\n<td style=\"background-color:#ffffff;border:0px;\"><a href=\"#a\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow.png\" width=\"16\" height=\"16\" border=\"0\" \/>\u00a0\u8981\u65e8<\/a><\/td>\n<td style=\"background-color:#ffffff;border:0px;\"><a href=\"#b\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow.png\" width=\"16\" height=\"16\" border=\"0\" \/>\u00a0\u306f\u3058\u3081\u306b<\/a><\/td>\n<td style=\"background-color:#ffffff;border:0px;\"><a href=\"#c\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow.png\" width=\"16\" height=\"16\" border=\"0\" \/>\u00a0\u6750\u6599\u3068\u65b9\u6cd5<\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"background-color:#ffffff;border:0px;width: 20%;\"><\/td>\n<td style=\"background-color:#ffffff;border:0px;\"><a href=\"#d\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow.png\" width=\"16\" height=\"16\" border=\"0\" \/>\u00a0\u7d50\u679c<\/a><\/td>\n<td style=\"background-color:#ffffff;border:0px;\"><a href=\"#e\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow.png\" width=\"16\" height=\"16\" border=\"0\" \/>\u00a0\u8003\u5bdf<\/a><\/td>\n<td style=\"background-color:#ffffff;border:0px;\"><a href=\"#f\"><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow.png\" width=\"16\" height=\"16\" border=\"0\" \/>\u00a0\u6587\u732e\u53ca\u3073\u53c2\u7167<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<blockquote>\n<p style=\"text-align: center;\">\u4e2d\u6751\u5e78\u592b1\u3001\u5409\u91ce\u4f73\u7e541,\u3000\u5ca9\u702c\u3000\u79c02\u3001\u98ef\u6751\u6075\u7f8e1\u3001\u897f\u689d\u3000\u85ab1\u3001\u6df1\u6d77\u3000\u85ab2\u3001\u5927\u91ce\u5fe0\u592b1\u3001\u5c0f\u5e61\u88d5\u4e003<\/p>\n<p style=\"text-align: center;\">1\u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc\u7d30\u80de\u6750\u6599\u958b\u767a\u5ba4\u30012\u60c5\u5831\u89e3\u6790\u6280\u8853\u5ba4\u30013\u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc<\/p>\n<\/blockquote>\n<p><a name=\"a\"><\/a><\/p>\n<hr \/>\n<h3 style=\"text-align: left;\">\u8981\u65e8<\/h3>\n<p style=\"text-align: right;\"><a href=\"#top\">TOP\u25b2<\/a><\/p>\n<p>\u00a0\u00a0\u00a0\u7d30\u80de\u6750\u6599\u3092\u7528\u3044\u305f\u7814\u7a76\u6210\u679c\u304c\u518d\u73fe\u6027\u306e\u3042\u308b\u79d1\u5b66\u7684\u306a\u3082\u306e\u3067\u3042\u308b\u305f\u3081\u306b\u306f\u3001\u7528\u3044\u305f\u7d30\u80de\u6750\u6599\u306e\u8d77\u6e90\u3001\u751f\u7269\u5b66\u7684\u7279\u6027\u7b49\u304c\u660e\u78ba\u306b\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3042\u308a\u3001\u7d30\u80de\u30d0\u30f3\u30af\u3067\u306f\u3001\u54c1\u8cea\u7ba1\u7406\u306b\u52aa\u3081\u3066\u304d\u305f\u3002 \u305d\u306e\u306a\u304b\u3067\u3082\u3001\u7d30\u80de\u306e\u8d77\u6e90\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u306f\u6700\u91cd\u8981\u4e8b\u9805\u3067\u3042\u308b\u3053\u3068\u3068\u8a8d\u8b58\u3057\u3001\u305d\u306e\u89e3\u6790\u65b9\u6cd5\u306e\u958b\u767a\u3092\u9032\u3081\u3066\u304d\u305f\u3002 \u907a\u4f1d\u5b50\u591a\u578b\u89e3\u6790\u306f\u7d30\u80de\u6750\u6599\u306e\u8d77\u6e90\u3092\u78ba\u8a8d\u3059\u308b\u4e0a\u3067\u6709\u7528\u306a\u89e3\u6790\u65b9\u6cd5\u306e\u4e00\u3064\u3067\u3042\u308b\u3002 \u3044\u304f\u3064\u304b\u306e\u65b9\u6cd5\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u304c\u3001\u305d\u306e\u4e2d\u3067\u3001\u6700\u8fd1\u958b\u767a\u3055\u308c\u305fShort Tandem Repeat-Polymerase Chain Reaction (STR-PCR) \u6cd5\u306f\u3001\u7c21\u4fbf\u3067\u4fe1\u983c\u6027\u306e\u9ad8\u3044\u89e3\u6790\u65b9\u6cd5\u3067\u3042\u308a\u3001\u591a\u6570\u306e\u8a66\u6599\u3092\u5bfe\u8c61\u3068\u3057\u305f\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306a\u89e3\u6790\u304c\u53ef\u80fd\u3067\u3042\u308a\u3001\u7d30\u80de\u30d0\u30f3\u30af\u304c\u6240\u6709\u3059\u308b\u591a\u6570\u306e\u7d30\u80de\u682a\u306e\u8d77\u6e90\u3092\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u3063\u305f\u3002 \u305d\u3053\u3067\u30012002\u5e74\u3088\u308a\u4e88\u5099\u5b9f\u9a13\u3092\u958b\u59cb\u3057\u3001\u305d\u306e\u6709\u52b9\u6027\u3092\u691c\u8a0e\u30fb\u78ba\u8a8d\u3057\u305f\u5f8c\u3001\u6211\u3005\u306e\u30bb\u30f3\u30bf\u30fc\u304c\u4fdd\u6709\u3057\u3001\u30ab\u30bf\u30ed\u30b0\u53ca\u3073\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306b\u63b2\u8f09\u3057\u3066\u3044\u308b\u5168\u30d2\u30c8\u7531\u6765\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066STR-PCR\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u30012005\u5e743\u6708\u306b\u5b9f\u9a13\u53ca\u3073\u5b9f\u9a13\u7d50\u679c\u306e\u89e3\u6790\u3092\u7d42\u4e86\u3057\u305f\u3002 \u89e3\u6790\u306b\u306f\u5bc4\u8a17\u5f53\u521d\u306b\u4fdd\u5b58\u3055\u308c\u305f\u7d30\u80de\u3092\u7528\u3044\u305f\u3002 \u7d50\u679c\u3068\u3057\u3066\u3001\u5bc4\u8a17\u6642\u70b9\u3067\u300c\u4ed6\u306e\u7d30\u80de\u304c\u6df7\u5165\u3057\u3001\u6df7\u5165\u3057\u305f\u7d30\u80de\u306b\u7f6e\u63db\u3055\u308c\u3066\u3044\u305f\u300d\u307e\u305f\u306f\u300c\u4ed6\u306e\u7d30\u80de\u3068\u306e\u53d6\u308a\u9055\u3048\u304c\u3042\u3063\u305f\u300d\u3068\u8003\u3048\u3089\u308c\u308b\u7d30\u80de\u682a\u304c7\u682a\u3042\u3063\u305f\u3002 \u307e\u305f\u3001\u540c\u4e00\u306e\u60a3\u8005\u7531\u6765\u306e\u7d30\u80de\u682a\u3068\u3057\u3066\u5bc4\u8a17\u3092\u53d7\u3051\u3066\u3044\u305f\u304c\u3001\u4e21\u7d30\u80de\u682a\u306f\u5225\u3005\u306e\u60a3\u8005\u7531\u6765\u306e\u7d30\u80de\u682a\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\u7d30\u80de\u682a\u306e\u7d44\u307f\u5408\u308f\u305b\u304c1\u7d44\u3042\u3063\u305f\u3002 \u3053\u308c\u3089\u306e\u53d6\u308a\u9055\u3048\u7d30\u80de\u307e\u305f\u306f\u8d77\u6e90\u4e0d\u660e\u7d30\u80de\u306f\u767b\u9332\u3092\u62b9\u6d88\u3057\u3001\u63d0\u4f9b\u3092\u4e2d\u6b62\u3057\u305f\u3002 \u4eca\u56de\u3001\u73fe\u6709\u306e\u7d30\u80de\u682a\u306b\u95a2\u3059\u308b\u89e3\u6790\u3092\u4e00\u901a\u308a\u7d42\u4e86\u3057\u305f\u304c\u3001\u4eca\u5f8c\u65b0\u898f\u306b\u53ce\u96c6\u3059\u308b\u7d30\u80de\u8a66\u6599\u3092\u542b\u3081\u3066\u3001\u30d2\u30c8\u7531\u6765\u7d30\u80de\u8a66\u6599\u306b\u95a2\u3057\u3066\u306f\u3001\u53ce\u96c6\u6642\u70b9\u53ca\u3073\u305d\u306e\u5f8c\u306e\u7d99\u4ee3\u57f9\u990a\u306e\u904e\u7a0b\u3067\u3001STR-PCR\u89e3\u6790\u3092\u7d99\u7d9a\u7684\u306b\u5b9f\u65bd\u3057\u3066\u3044\u304f\u4e88\u5b9a\u3067\u3042\u308b\u3002 \u7279\u306b\u3001\u65b0\u898f\u306b\u63d0\u4f9b\u3092\u958b\u59cb\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u8d77\u6e90\u304c\u78ba\u8a8d\u3055\u308c\u305f\u7d30\u80de\u682a\u306e\u307f\u3092\u5bfe\u8c61\u3068\u3059\u308b\u3053\u3068\u3068\u3059\u308b\u3002<\/p>\n<p><a name=\"b\"><\/a><\/p>\n<hr \/>\n<h3>\u306f\u3058\u3081\u306b<\/h3>\n<p style=\"text-align: right;\"><a href=\"#top\">TOP\u25b2<\/a><\/p>\n<p>\u57f9\u990a\u7d30\u80de\u682a\u306f\u7814\u7a76\u6750\u6599\u3068\u3057\u3066\u5fc5\u9808\u306a\u3082\u306e\u3067\u3042\u308a\u3001\u81a8\u5927\u306a\u7a2e\u985e\u306e\u57f9\u990a\u7d30\u80de\u682a\u304c\u5e83\u304f\u591a\u304f\u306e\u7814\u7a76\u8005\u306b\u3088\u3063\u3066\u5b9f\u9a13\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u3002 \u57f9\u990a\u7d30\u80de\u682a\u306f\u7dad\u6301\u3068\u4f7f\u7528\u306e\u305f\u3081\u306b\u3001\u7d99\u4ee3\u57f9\u990a\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001\u305d\u306e\u904e\u7a0b\u3067\u4ed6\u306e\u7d30\u80de\u304c\u6df7\u5165\u3057\u305f\u308a\u3001\u4ed6\u306e\u7d30\u80de\u3068\u53d6\u308a\u9055\u3048\u308b\u3068\u3044\u3046\u4eba\u70ba\u7684\u30df\u30b9\u306f\u5b8c\u5168\u306b\u306f\u9632\u304e\u304d\u308c\u306a\u3044\u3053\u3068\u3067\u3042\u308b\u3002 \u7d50\u679c\u3068\u3057\u3066\u3001\u6df7\u5165\u3057\u305f\u7d30\u80de\u304c\u512a\u52e2\u306b\u5897\u6b96\u3057\u3066\u5b8c\u5168\u306b\u7f6e\u304d\u63db\u3063\u3066\u3057\u307e\u3063\u305f\u308a\u3001\u53d6\u308a\u9055\u3048\u306b\u3088\u3063\u3066\u5168\u304f\u5225\u306e\u7d30\u80de\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u3068\u3044\u3046\u4e8b\u614b\u304c\u767a\u751f\u3059\u308b\u3002 \u3053\u3046\u3057\u305f\u4e8b\u614b\u306e\u767a\u751f\u306f\u3001\u53e4\u304f\u304b\u3089\u61f8\u5ff5\u3055\u308c\u3001\u6307\u6458\u3055\u308c\u3066\u3044\u305f\u3068\u3053\u308d\u3067\u3042\u3063\u305f\uff081\uff09\u3002 \u3057\u304b\u3057\u3001\u57f9\u990a\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u3001\u4ed6\u306e\u7d30\u80de\u306e\u6df7\u5165\u3084\u53d6\u308a\u9055\u3048\u304c\u7121\u3044\u304b\u5426\u304b\u306e\u691c\u5b9a\u306f\u3001\u5f93\u6765\u306e\u5f62\u614b\u5b66\u7684\u89b3\u5bdf\u3084\u67d3\u8272\u4f53\u691c\u67fb\u7b49\u306e\u307f\u3067\u306f\u4e0d\u53ef\u80fd\u3067\u3042\u3063\u305f\u3002<\/p>\n<p>\u7d30\u80de\u682a\u306e\u8d77\u6e90\u3092\u78ba\u5b9f\u306b\u691c\u5b9a\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u6700\u7d42\u7684\u306b\u306f\u907a\u4f1d\u5b50\u30ec\u30d9\u30eb\u3067\u306e\u591a\u578b\u89e3\u6790\u304c\u6709\u52b9\u3067\u3042\u308b\u304c\u3001\u81a8\u5927\u306a\u7a2e\u985e\u306e\u57f9\u990a\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u3001\u305d\u306e\u5168\u7d30\u80de\u306e\u8a73\u7d30\u306a\u907a\u4f1d\u5b50\u591a\u578b\u89e3\u6790\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u306f\u73fe\u5b9f\u7684\u306b\u306f\u4e0d\u53ef\u80fd\u3067\u3042\u308b\u3002 \u6700\u8fd1\u958b\u767a\u3055\u308c\u305fDNA finger print \u89e3\u6790\u6cd5\u306e\u4e00\u7a2e\u3067\u3042\u308bMultiplex Short Tandem Repeat-Polymerase Chain Reaction (STR-PCR) \u6cd5\u306f\u3001\u30b2\u30ce\u30e0\u4e0a\u306e\u69d8\u3005\u306a\u90e8\u4f4d\u306b\u5b58\u5728\u3059\u308b\u7e70\u8fd4\u3057\u914d\u5217\uff08Short Tandem Repeat\uff09\u306f\u305d\u306e\u7e70\u8fd4\u3057\u56de\u6570\u306b\u95a2\u3057\u3066\u591a\u578b\u6027\u304c\u3042\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u305f\u907a\u4f1d\u5b50\u591a\u578b\u89e3\u6790\u6cd5\u3067\u3042\u308a\u3001\u3044\u304f\u3064\u304b\u306e\u9818\u57df\u306e\u89e3\u6790\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\uff08Multiplex\uff09\u7d71\u8a08\u5b66\u7684\u691c\u5b9a\u306e\u7cbe\u5ea6\u3092\u9ad8\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff082\uff09\u3002 Multiplex STR-PCR\u6cd5\u306f\u3001\u7c21\u4fbf\u3067\u4fe1\u983c\u6027\u306e\u9ad8\u3044\u907a\u4f1d\u5b50\u591a\u578b\u89e3\u6790\u6cd5\u3067\u3042\u308a\u3001\u81a8\u5927\u306a\u8a66\u6599\u3092\u5bfe\u8c61\u3068\u3057\u305f\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306a\u89e3\u6790\u3092\u53ef\u80fd\u3068\u3057\u305f\u3002 \u3057\u304b\u3057\u3001\u57f9\u990a\u7d30\u80de\u682a\u306e\u591a\u304f\u306f\u67d3\u8272\u4f53\u304c3\u500d\u4f53\u30014\u500d\u4f53\u3042\u308b\u3044\u306f\u305d\u308c\u4ee5\u4e0a\u306b\u5909\u7570\u3057\u3066\u3044\u308b\u3053\u3068\u3001\u307e\u305f\u67d3\u8272\u4f53\u306e\u6b20\u5931\u5909\u7570\u3001\u633f\u5165\u5909\u7570\u3001\u8ee2\u5ea7\u7b49\u3082\u983b\u7e41\u306b\u767a\u751f\u3057\u3066\u3044\u308b\u3053\u3068\u7b49\u304b\u3089\u3001\u7d30\u80de\u682a\u306e\u8d77\u6e90\u306e\u78ba\u8a8d\u691c\u67fb\u6cd5\u3068\u3057\u3066Multiplex STR-PCR\u6cd5\u306e\u9069\u7528\u304c\u53ef\u80fd\u304b\u5426\u304b\u306e\u691c\u8a3c\u304c\u5fc5\u8981\u3067\u3042\u3063\u305f\u3002 \u3057\u304b\u3057\u3001\u305d\u306e\u5f8c\u306e\u691c\u8a3c\u5b9f\u9a13\u306b\u3088\u308a\u3001\u3053\u306e\u65b9\u6cd5\u304c\u7d30\u80de\u682a\u306e\u8d77\u6e90\u306e\u78ba\u8a8d\u691c\u67fb\u6cd5\u3068\u3057\u3066\u4fe1\u983c\u6027\u306e\u9ad8\u3044\u6709\u52b9\u306a\u65b9\u6cd5\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\uff083\uff09\u3002 \u3055\u3089\u306b\u3001\u4e16\u754c\u306e\u5404\u7d30\u80de\u30d0\u30f3\u30af\u306b\u304a\u3044\u3066\u3082\u72ec\u81ea\u306b\u691c\u8a0e\u304c\u91cd\u306d\u3089\u308c\u3001\u540c\u6cd5\u304c\u6709\u52b9\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u3064\u3064\u3042\u308b\u3002 \u5f53\u5ba4\u306b\u304a\u3044\u3066\u3082\u3001Multiplex STR-PCR\u6cd5\u306e\u6709\u7528\u6027\u3092\u691c\u8a0e\u30fb\u5b9f\u8a3c\u3057\u3001\u4fdd\u6709\u30d2\u30c8\u7531\u6765\u7d30\u80de\u306b\u95a2\u3059\u308b\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304d\u305f\u3002 \u6700\u7d42\u7684\u306b\u3001\u30ab\u30bf\u30ed\u30b0\u53ca\u3073\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306b\u63b2\u8f09\u3057\u3066\u3044\u308b\u5168\u30d2\u30c8\u7531\u6765\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u3001\u5bc4\u8a17\u5f53\u521d\u306b\u4fdd\u5b58\u3055\u308c\u305f\u7d30\u80de\u3092\u7528\u3044\u3066\u3001Multiplex STR-PCR\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u3001\u6700\u8fd1\u305d\u306e\u89e3\u6790\u3092\u7d42\u4e86\u3057\u305f\u306e\u3067\u3001\u3053\u3053\u306b\u305d\u306e\u7d50\u679c\u3092\u5831\u544a\u3059\u308b\u3002<\/p>\n<p><a name=\"c\"><\/a><\/p>\n<hr \/>\n<h3>\u6750\u6599\u3068\u65b9\u6cd5<\/h3>\n<p style=\"text-align: right;\"><a href=\"#top\">TOP\u25b2<\/a><\/p>\n<p><span style=\"color: #008000;\"><em>\u7d30\u80de<\/em><\/span><\/p>\n<p style=\"padding-left: 30px;\">\u00a0\u5f53\u30bb\u30f3\u30bf\u30fc\u306e\u30ab\u30bf\u30ed\u30b0\u53ca\u3073\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306b\u63b2\u8f09\u3057\u3066\u3044\u308b\u5168\u30d2\u30c8\u7531\u6765\u7d30\u80de\u682a\u3092\u5bfe\u8c61\u3068\u3057\u305f\u3002 \u5b9f\u9a13\u306b\u306f\u3001\u5bc4\u8a17\u5f53\u521d\u306b\u4fdd\u5b58\u3055\u308c\u305f\u7d30\u80de\u3092\u7528\u3044\u305f\u3002<\/p>\n<p>\u00a0<em><span style=\"color: #008000;\">DNA\u62bd\u51fa\u6cd5<\/span><\/em><\/p>\n<p style=\"padding-left: 30px;\">\u00a0\u00a02&#215;10<sup>6<\/sup>\u500b\u306e\u7d30\u80de\u304b\u3089\u3001DNeasy Tissue Kit<sup>R<\/sup> (QIAGEN\u3001Hilden) \u3092\u7528\u3044\u3066\u3001DNA\u3092\u62bd\u51fa\u3057\u305f\u3002<\/p>\n<p><span style=\"color: #008000;\"><em>Multiplex STR-PCR\u6cd5<\/em><\/span><\/p>\n<p style=\"padding-left: 30px;\">Promega PowerPlex<sup>R<\/sup>1.2 \u3092\u7528\u3044\u3066\u89e3\u6790\u3092\u884c\u3063\u305f\u3002 DNA 2.5 ng\u3092\u92f3\u578b\u3068\u3057\u3001AmpliTaq Gold<sup>R<\/sup> (Applied Biosystems, California) \u3092\u7528\u3044\u3066PCR\u3092\u884c\u3063\u305f\u3002 \u7528\u3044\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306f<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table1\">\u88681<\/a>\u306b\u793a\u3059\u3002 PCR\u306b\u306fGeneAmp<sup>R<\/sup> PCR system 9700\uff08Applied Biosystems\uff09\u3092\u7528\u3044\u3001\u6761\u4ef6\u306fdenature 94\u2103 30 sec, annealing 60\u2103 30 sec, extension 70\u2103 45 sec \u306b\u306610 cycle, \u6b21\u306bdenature 90\u2103 30 sec, annealing 60\u2103 30 sec, extension 70\u2103 45 sec \u306b\u306620 cycle\u3067\u5b9f\u65bd\u3057\u305f\u3002 PCR\u5f8c\u306e\u30b5\u30f3\u30d7\u30eb\u3092deionized formamide\u306b\u6eb6\u89e3\u3057\u3001ABI PRISM<sup>TM<\/sup> 310 Genetic Analyzer\uff08Applied Biosystems\uff09\u306b\u3066PCR\u7523\u7269\u306e\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u305f\u3002<\/p>\n<p><em><span style=\"color: #008000;\">\u7d71\u8a08\u5b66\u7684\u89e3\u6790\u6cd5<\/span><\/em><\/p>\n<p style=\"padding-left: 30px;\">\u7570\u306a\u308b\uff19\u7b87\u6240\u306e\u67d3\u8272\u4f53\u9818\u57df\u306b\u304a\u3051\u308b\u3001\u305d\u308c\u305e\u308c\u306e\u7e70\u8fd4\u3057\u914d\u5217\u306e\u7e70\u8fd4\u3057\u56de\u6570\u306e\u7d50\u679c\u3092\u3001\u89e3\u6790\u3057\u305f\u4ed6\u306e\u5168\u3066\u306e\u7d30\u80de\u306b\u5bfe\u3057\u3066\u6bd4\u8f03\u3057\u3001\u591a\u6b21\u5143\u89e3\u6790\u3092\u884c\u3044\u3001\u968e\u5c64\u7684\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\uff08hierarchical clustering method\uff09\u306e\u6700\u77ed\u8ddd\u96e2\u6cd5\uff08euclidean\uff09\u3067\u89e3\u6790\u3057\u305f\uff084\uff09\u3002 \u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u624b\u6cd5\u3068\u3057\u3066\u306f\u3001\u975e\u968e\u5c64\u7684\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\uff08non-hierarchical clustering method\uff09\u306e\u3046\u3061\u306e\u4ee3\u8868\u7684\u306a k-mean \u6cd5\u53ca\u3073 Fuzzy cMeans \u6cd5\uff084\uff09\u3082\u8a66\u307f\u305f\u304c\u3001\u65e2\u77e5\u306e\u60c5\u5831\u3068\u5b9f\u9a13\u30c7\u30fc\u30bf\u3092\u6bd4\u8f03\u691c\u8a0e\u3057\u305f\u7d50\u679c\u3001\u968e\u5c64\u7684\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u306e\u65b9\u304c\u9069\u3057\u3066\u3044\u308b\u3068\u5224\u65ad\u3057\u3001\u968e\u5c64\u7684\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u3092\u7528\u3044\u3066\u89e3\u6790\u3057\u305f\u3002 \u78ba\u7387\u5206\u5e03\u304b\u3089\u30ef\u30a4\u30d6\u30eb\u5206\u5e03\u306b\u9069\u5408\u3055\u305b\u300199.5\uff05\u4fe1\u983c\u533a\u9593\u3092\u8d8a\u3048\u308b\u3082\u306e\u3092\u3001\u540c\u4e00\u7d30\u80de\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3057\u305f\uff084\uff09\u3002<\/p>\n<p><a name=\"d\"><\/a><\/p>\n<hr \/>\n<h3>\u7d50\u679c<\/h3>\n<p style=\"text-align: right;\"><a href=\"#top\">TOP\u25b2<\/a><\/p>\n<p>Multiplex STR-PCR\u89e3\u6790\u306b\u95a2\u3057\u3066\u30012002\u5e74\u3088\u308a\u4e88\u5099\u5b9f\u9a13\u3092\u958b\u59cb\u3057\u3001\u305d\u306e\u6709\u52b9\u6027\u3092\u691c\u8a0e\u30fb\u78ba\u8a8d\u3057\u305f\u5f8c\u3001\u6211\u3005\u306e\u30bb\u30f3\u30bf\u30fc\u304c\u4fdd\u6709\u3057\u3001\u30ab\u30bf\u30ed\u30b0\u53ca\u3073\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306b\u63b2\u8f09\u3057\u3066\u3044\u308b\u5168\u30d2\u30c8\u7531\u6765\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066STR-PCR\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u30012005\u5e743\u6708\u306b\u5b9f\u9a13\u53ca\u3073\u5b9f\u9a13\u7d50\u679c\u306e\u89e3\u6790\u3092\u7d42\u4e86\u3057\u305f\u3002 \u89e3\u6790\u306f\u3001\u500b\u3005\u306e\u7d30\u80de\u682a\u306e\u30b2\u30ce\u30e0DNA\u3092\u92f3\u578b\u3068\u3057\u3066\uff19\u7a2e\u985e\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u3092\u7528\u3044\u3066\u5b9f\u65bd\u3055\u308c\u305fPCR\u306e\u7d50\u679c\u306b\u95a2\u3057\u3066\u3001\u4ed6\u306e\u5168\u3066\u306e\u7d30\u80de\u306e\u5b9f\u9a13\u7d50\u679c\u3068\u6bd4\u8f03\u691c\u8a0e\u3059\u308b\u3068\u3044\u3046\u65b9\u6cd5\u3067\u884c\u3063\u305f\u3002 \u5404\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306b\u3088\u3063\u3066\u51fa\u73fe\u3059\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b\u30d4\u30fc\u30af\u306e\u5168\u7a2e\u985e\u3092\u691c\u51fa\u3067\u304d\u308b\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u5b9f\u9a13\u306e\u7d50\u679c\u3092<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_figure1\">\u56f3\uff11<\/a>\u306b\u793a\u3059\u3002 \u5b9f\u969b\u306e\u8a66\u6599\u3067\u306f\u3001\u5404\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306b\u3088\u3063\u3066\u3001\u539f\u5247\u7684\u306b\u306f\u30d4\u30fc\u30af\u304c\uff11\u672c\u307e\u305f\u306f\uff12\u672c\u691c\u51fa\u3055\u308c\u308b\u3053\u3068\u306b\u306a\u308b\u3002 \u7236\u89aa\u30fb\u6bcd\u89aa\u53cc\u65b9\u304b\u3089\u7e70\u308a\u8fd4\u3057\u6570\u304c\u540c\u3058\u907a\u4f1d\u5b50\u578b\u3092\u5f15\u304d\u7d99\u3044\u3067\u3044\u308b\u3068\uff11\u672c\u306e\u30d4\u30fc\u30af\u3001\u7236\u89aa\u30fb\u6bcd\u89aa\u304b\u3089\u7570\u306a\u308b\u7e70\u308a\u8fd4\u3057\u6570\u306e\u907a\u4f1d\u5b50\u578b\u3092\u5f15\u304d\u7d99\u3044\u3067\u3044\u308b\u3068\uff12\u672c\u306e\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u308b\u3002 \u5404\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u306b\u3088\u3063\u3066\u3069\u306e\u9577\u3055\u306e\u30d4\u30fc\u30af\u304c\u691c\u51fa\u3055\u308c\u305f\u304b\u306e\u7d50\u679c\u306b\u95a2\u3059\u308b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u69cb\u7bc9\u3057\u3001\u7d71\u8a08\u5b66\u7684\u51e6\u7406\u306e\u5f8c\u3001\u78ba\u7387\u5206\u5e03\u304b\u3089\u30ef\u30a4\u30d6\u30eb\u5206\u5e03\u306b\u9069\u5408\u3055\u305b\u300199.5\uff05\u4fe1\u983c\u533a\u9593\u3092\u8d8a\u3048\u308b\u3082\u306e\u3092\u3001\u540c\u4e00\u7d30\u80de\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3057\u305f\uff08\u6750\u6599\u3068\u65b9\u6cd5\u3092\u53c2\u7167\uff09\u3002<\/p>\n<p><em><span style=\"color: #008000;\">\u7570\u306a\u308b\u7814\u7a76\u8005\u304b\u3089\u5bc4\u8a17\u3055\u308c\u305f\u7d30\u80de\u682a\u304c\u540c\u4e00\u7d30\u80de\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\u7d30\u80de\u30da\u30a2<\/span><\/em><\/p>\n<p style=\"padding-left: 30px;\"><strong><\/strong>\u00a0\u00a0RERF-LC-AI\u7d30\u80de\uff08\u80ba\u764c\u7531\u6765\u7d30\u80de\u682a\uff09\u3068GT3TKB\u7d30\u80de\uff08\u80c3\u764c\u7531\u6765\u7d30\u80de\u682a\uff09\u3068\u304c\u540c\u4e00\u306e\u7d30\u80de\u682a\u3067\u3042\u308b\u3053\u3068\u3092\u5f37\u304f\u793a\u5506\u3059\u308b\u7d50\u679c\u304c\u5f97\u3089\u308c\u305f\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table2\">\u88682<\/a>\uff09\u3002 \u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u682a\u306e\u5bc4\u8a17\u8005\u306f\u7570\u306a\u308b\u304c\u3001GT3TKB\u7d30\u80de\u306e\u5bc4\u8a17\u8005\u3088\u308a \u300cGT3TKB\u7d30\u80de\u3068RERF-LC-AI\u7d30\u80de\u3068\u3092\u540c\u6642\u306b\u57f9\u990a\u3057\u3066\u3044\u305f\u6642\u671f\u304c\u3042\u308a\u3001GT3TKB\u7d30\u80de\u3078\u306eRERF-LC-AI\u7d30\u80de\u306e\u6df7\u5165\u307e\u305f\u306f\u53d6\u308a\u9055\u3048\u304c\u3042\u3063\u305f\u53ef\u80fd\u6027\u304c\u9ad8\u304f\u3001GT3TKB\u7d30\u80de\u306e\u5bc4\u8a17\u3092\u53d6\u4e0b\u3052\u308b\u3002\u300d \u3068\u3044\u3046\u8da3\u65e8\u306e\u9023\u7d61\u3092\u53d7\u3051\u305f\u3002 RERF-LC-AI\u7d30\u80de\u306e\u63d0\u4f9b\u306f\u7d99\u7d9a\u3057\u3001GT3TKB\u7d30\u80de\u306e\u63d0\u4f9b\u306f\u4e2d\u6b62\u3057\u305f\u3002<\/p>\n<p style=\"padding-left: 30px;\">\u00a0\u00a0\u305d\u306e\u4ed6\u3001\u5f53\u30bb\u30f3\u30bf\u30fc\u3078\u306e\u5bc4\u8a17\u4ee5\u524d\u306b\u3001\u300cHeLa\u7d30\u80de\u304c\u6df7\u5165\u3057\u3001HeLa\u7d30\u80de\u304c\u512a\u52e2\u306b\u5897\u6b96\u3057\u305f\u300d\u307e\u305f\u306f\u300cHeLa\u7d30\u80de\u3068\u306e\u53d6\u308a\u9055\u3048\u304c\u3042\u3063\u305f\u300d\u3068\u5224\u65ad\u3055\u308c\u305f\u7d30\u80de\u682a\u304c2\u7a2e\u985e\u3042\u3063\u305f\u3002 \u305d\u308c\u3089\u306e\u7d30\u80de\u682a\u306e\u63d0\u4f9b\u306f\u73fe\u5728\u4e2d\u6b62\u3057\u3066\u3044\u308b\u3002<\/p>\n<p><em><span style=\"color: #008000;\">\u00a0\u540c\u3058\u7814\u7a76\u8005\u304b\u3089\u7570\u306a\u308b\u7d30\u80de\u682a\u3068\u3057\u3066\u5bc4\u8a17\u3055\u308c\u305f\u682a\u304c\u540c\u4e00\u7d30\u80de\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\u7d30\u80de\u30da\u30a2<\/span><\/em><\/p>\n<p style=\"padding-left: 30px;\">\u300cSCCTF\u7d30\u80de\u3068SCCKN\u7d30\u80de\u300d\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table3\">\u88683<\/a>\uff09\u3001\u300cLu-130\u7d30\u80de\u3068Lu-134(A,B)\u7d30\u80de\u300d\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table4\">\u88684<\/a>\uff09\u3001\u300cHKMUS-SF\u7d30\u80de\u3068SKG-II-SF\u7d30\u80de\u300d\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table5\">\u88685<\/a>\uff09\u3001\u300cETK-1\u7d30\u80de\u3068SSP-25\u7d30\u80de\u300d\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table6\">\u88686<\/a>\uff09\u3001\u300cTCO-1\u7d30\u80de\u3068TCS\u7d30\u80de\u300d\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table7\">\u88687<\/a>\uff09\u3001\u4ee5\u4e0a\u306f\u3044\u305a\u308c\u3082\u540c\u3058\u7814\u7a76\u8005\u304b\u3089\u3001\u7570\u306a\u308b\u60a3\u8005\u304b\u3089\u6a39\u7acb\u3055\u308c\u305f\u540c\u3058\u7a2e\u985e\u306e\u764c\u306e\u7d30\u80de\u682a\u3068\u3057\u3066\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u30da\u30a2\u3067\u3042\u308b\u3002 \u3057\u304b\u3057\u3001\u4eca\u56de\u306e\u691c\u67fb\u3067\u305d\u308c\u305e\u308c\u540c\u4e00\u60a3\u8005\u7531\u6765\u306e\u7d30\u80de\u682a\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3059\u7d50\u679c\u304c\u5f97\u3089\u308c\u305f\u3002 \u4eca\u5f8c\u306e\u5bfe\u5fdc\u306b\u95a2\u3057\u3066\u306f\u3001\u5bc4\u8a17\u8005\u3068\u3082\u76f8\u8ac7\u306e\u4e0a\u3001\u8868\u4e2d\u306b\u793a\u3057\u3066\u3042\u308b\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table8\">\u88688<\/a>\uff09\u3002<\/p>\n<p>\u00a0<em><span style=\"color: #008000;\">\u540c\u4e00\u60a3\u8005\u7531\u6765\u306e\u7d30\u80de\u682a\u3067\u3042\u308b\u3068\u3055\u308c\u3066\u3044\u305f\u304c\u7570\u306a\u308b\u60a3\u8005\u7531\u6765\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\u7d30\u80de\u682a<\/span><\/em><\/p>\n<p style=\"padding-left: 30px;\">TCO-1\u7d30\u80de\u3068TCO-2\u7d30\u80de\u3068\u306f\u3001\u540c\u4e00\u60a3\u8005\u306e\u5b50\u5bae\u764c\u7531\u6765\u306e\u7d30\u80de\u682a\u3067\u3042\u308b\u3068\u3057\u3066\u540c\u4e00\u7814\u7a76\u8005\u304b\u3089\u5bc4\u8a17\u3055\u308c\u3066\u3044\u305f\u304c\u3001\u4e21\u7d30\u80de\u682a\u306f\u7570\u306a\u308b\u60a3\u8005\u7531\u6765\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table7\">\u88687<\/a>\uff09\u3002 \u5bc4\u8a17\u8005\u306e\u610f\u5411\u306b\u3088\u308a\u3001TCO-2\u7d30\u80de\u306e\u307f\u306e\u63d0\u4f9b\u3092\u7d99\u7d9a\u3059\u308b\u3002<\/p>\n<p>\u00a0<span style=\"color: #008000;\"><em>\u5973\u6027\u7531\u6765\u7d30\u80de\u3067\u3042\u308b\u3068\u3055\u308c\u3066\u3044\u305f\u304cY\u67d3\u8272\u4f53\u304c\u691c\u51fa\u3055\u308c\u305f\u7d30\u80de\u682a<\/em><\/span><\/p>\n<p style=\"padding-left: 30px;\">\u5973\u6027\u4e73\u764c\u7d30\u80de\u7531\u6765\u3067\u3042\u308b\u3068\u3057\u3066\u5bc4\u8a17\u3055\u308c\u3066\u3044\u305fHBL-100\u7d30\u80de\u306b\u3001\uff39\u67d3\u8272\u4f53\u306e\u5b58\u5728\u304c\u5f37\u304f\u793a\u5506\u3055\u308c\u305f\u305f\u3081\u3001FISH (fluorescence in situ hybridization)\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u305f\u3068\u3053\u308d\u3001\uff39\u67d3\u8272\u4f53\u306e\u5b58\u5728\u304c\u78ba\u8a8d\u3055\u308c\u305f\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_figure2\">\u56f32<\/a>\uff09\u3002 \u5bc4\u8a17\u8005\u304b\u3089\u306e\u5bc4\u8a17\u53d6\u4e0b\u3052\u306e\u610f\u601d\u3092\u78ba\u8a8d\u3057\u305f\u306e\u3067\u3001HBL-100\u7d30\u80de\u306e\u63d0\u4f9b\u306f\u4e2d\u6b62\u3057\u305f\u3002<\/p>\n<p><a name=\"e\"><\/a><\/p>\n<hr \/>\n<h3>\u8003\u5bdf<\/h3>\n<p style=\"text-align: right;\"><a href=\"#top\">TOP\u25b2<\/a><\/p>\n<p><strong>\u3000\u3000<\/strong>HeLa\u7d30\u80de\u306f\u4e16\u754c\u3067\u521d\u3081\u3066\u6a39\u7acb\u3055\u308c\u305f\u7d30\u80de\u682a\u3067\u3042\u308a\u3001\u305d\u306e\u5f8c\u591a\u6570\u306e\u7814\u7a76\u8005\u306b\u3088\u3063\u3066\u81a8\u5927\u306a\u6570\u306e\u5b9f\u9a13\u306b\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u3002 HeLa\u7d30\u80de\u306f\u3001\u73fe\u5728\u3067\u3082\u6700\u3082\u6c4e\u7528\u6027\u306e\u9ad8\u3044\u7d30\u80de\u682a\u306e\u4e00\u3064\u3067\u3042\u308a\u3001HeLa\u7d30\u80de\u306e\u4ed6\u306e\u7d30\u80de\u682a\u3078\u306e\u6df7\u5165\u306f\u3001\u53e4\u304f\u304b\u3089\u6307\u6458\u3055\u308c\u3066\u3044\u305f\uff083\uff09\u3002 \u4eca\u56de\u306e\u691c\u67fb\u3067\u306f\u30012\u7a2e\u985e\u306e\u7d30\u80de\u306b\u95a2\u3057\u3066\u3001HeLa\u7d30\u80de\u306e\u6df7\u5165\u306b\u3088\u308b\u7f6e\u63db\u304c\u751f\u3058\u3066\u3044\u305f\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3053\u3068\u304c\u5224\u660e\u3057\u305f\u3002<br \/>\n<strong>\u3000\u3000<\/strong>GT3TKB\u7d30\u80de\u306f\u3001\u540c\u7d30\u80de\u304c\u6a39\u7acb\u3055\u308c\u308b\u3088\u308a\u4ee5\u524d\u306b\u6a39\u7acb\u3055\u308c\u3066\u3044\u305fRERF-LC-AI\u7d30\u80de\u3068\u540c\u4e00\u306e\u7d30\u80de\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\u3002 \u300c\u7d50\u679c\u300d\u3067\u3082\u8ff0\u3079\u305f\u304c\u3001GT3TKB\u7d30\u80de\u306e\u6a39\u7acb\u30fb\u5bc4\u8a17\u8005\u3082\u3001\u6211\u3005\u304c\u4eca\u56de\u5b9f\u65bd\u3057\u305f\u691c\u67fb\u7d50\u679c\u304b\u3089\u5224\u65ad\u3057\u3066\u3001\u5bc4\u8a17\u5f53\u521d\u304b\u3089RERF-LC-AI\u7d30\u80de\u306e\u300c\u6df7\u5165\u306b\u3088\u308b\u7f6e\u63db\u300d\u307e\u305f\u306f\u300c\u53d6\u308a\u9055\u3048\u300d\u304c\u3042\u3063\u305f\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3053\u3068\u3092\u8a8d\u3081\u3001\u5bc4\u8a17\u3092\u53d6\u308a\u4e0b\u3052\u308b\u3053\u3068\u3068\u306a\u3063\u305f\u3002 \u3053\u308c\u4ee5\u5916\u3067\u540c\u4e00\u4eba\u7269\u7531\u6765\u7d30\u80de\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u5224\u65ad\u3055\u308c\u305f\u7d30\u80de\u30da\u30a2\u306f\u3001\u540c\u4e00\u306e\u5bc4\u8a17\u8005\u304b\u3089\u306e\u5bc4\u8a17\u7d30\u80de\u3067\u3042\u308a\u3001\u7531\u6765\u3059\u308b\u764c\u306e\u7a2e\u985e\u304c\u540c\u4e00\u306e\u3082\u306e\u3067\u3042\u3063\u305f\uff08<a href=\"\/ja\/quality\/str\/origin\/confirm_report\/confirm_table8\">\u88688<\/a>\uff09\u3002<br \/>\n<strong>\u3000\u3000<\/strong>HBL-100\u7d30\u80de\u306f\u5973\u6027\u4e73\u764c\u7531\u6765\u7d30\u80de\u3067\u3042\u308b\u3068\u3057\u3066\u5bc4\u8a17\u3092\u53d7\u3051\u3066\u3044\u305f\u304c\u3001\u4eca\u56de\u306e\u691c\u67fb\u3067\u7537\u6027\u7531\u6765\u7d30\u80de\u3067\u3042\u308b\u3053\u3068\u304c\u5224\u660e\u3057\u305f\u3002 \u300c\u6df7\u5165\u306b\u3088\u308b\u7f6e\u63db\u300d\u307e\u305f\u306f\u300c\u53d6\u308a\u9055\u3048\u300d\u3092\u8d77\u3053\u3057\u305f\u7d30\u80de\u306f\u3001\u5f53\u5ba4\u304c\u4fdd\u6709\u3059\u308b\u7d30\u80de\u682a\u306e\u4e2d\u306b\u306f\u5b58\u5728\u305b\u305a\u3001\u73fe\u6642\u70b9\u3067\u306f\u4e0d\u660e\u3067\u3042\u308b\u3002<br \/>\n<strong>\u3000\u3000<\/strong>Multiplex STR-PCR\u89e3\u6790\u306f\u3001\u5404\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u3001\u5bc4\u8a17\u5f53\u521d\u306e\u7d30\u80de\u3092\u7528\u3044\u3066\u5b9f\u65bd\u3057\u305f\u3002 \u5f93\u3063\u3066\u3001\u300c\u4ed6\u306e\u7d30\u80de\u306e\u6df7\u5165\u306b\u3088\u308b\u7f6e\u63db\u300d\u307e\u305f\u306f\u300c\u4ed6\u306e\u7d30\u80de\u3068\u306e\u53d6\u308a\u9055\u3048\u300d\u306f\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u6642\u70b9\u3067\u65e2\u306b\u5b58\u5728\u3057\u3066\u3044\u305f\u3068\u8003\u3048\u3089\u308c\u305f\u3002 \u4eca\u56de\u3001\u73fe\u6709\u306e\u7d30\u80de\u682a\u306b\u95a2\u3059\u308b\u89e3\u6790\u3092\u4e00\u901a\u308a\u7d42\u4e86\u3057\u305f\u304c\u3001\u4eca\u5f8c\u65b0\u898f\u306b\u53ce\u96c6\u3059\u308b\u7d30\u80de\u8a66\u6599\u3092\u542b\u3081\u3066\u3001\u30d2\u30c8\u7531\u6765\u7d30\u80de\u8a66\u6599\u306b\u95a2\u3057\u3066\u306f\u3001\u53ce\u96c6\u6642\u70b9\u53ca\u3073\u305d\u306e\u5f8c\u306e\u7d99\u4ee3\u57f9\u990a\u306e\u904e\u7a0b\u3067\u3001Multiplex STR-PCR\u89e3\u6790\u3092\u7d99\u7d9a\u7684\u306b\u5b9f\u65bd\u3057\u3066\u3044\u304f\u4e88\u5b9a\u3067\u3042\u308b\u3002 \u7279\u306b\u3001\u65b0\u898f\u306b\u63d0\u4f9b\u3092\u958b\u59cb\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u8d77\u6e90\u304c\u78ba\u8a8d\u3055\u308c\u305f\u7d30\u80de\u682a\u306e\u307f\u3092\u5bfe\u8c61\u3068\u3059\u308b\u3053\u3068\u3068\u3059\u308b\u3002<br \/>\n<strong>\u3000\u3000<\/strong>\u7d30\u80de\u682a\u306e\u6df7\u540c\u306e\u539f\u56e0\u3068\u3057\u3066\u306f\u3001\u4e00\u65b9\u306e\u7d30\u80de\u306e\u4ed6\u65b9\u3078\u306e\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3001\u57f9\u990a\u5bb9\u5668\u4e38\u3054\u3068\u306e\u53d6\u308a\u9055\u3048\u3001\u4ed6\u7814\u7a76\u8005\u3078\u306e\u8b72\u6e21\u306e\u969b\u306e\u52d8\u9055\u3044\u7b49\u3005\u69d8\u3005\u306a\u539f\u56e0\u304c\u8003\u3048\u3089\u308c\u308b\u3002 \u5f62\u614b\u3084\u5897\u6b96\u80fd\u3060\u3051\u3067\u306f\u533a\u5225\u304c\u56f0\u96e3\u306a\u7d30\u80de\u682a\u9593\u540c\u58eb\u3067\u306f\u3001\u3053\u3046\u3057\u305f\u6df7\u540c\u306f\u5c11\u306a\u304b\u3089\u305a\u767a\u751f\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002 \u30d2\u30c8\u7531\u6765\u7d30\u80de\u682a\u306e\u307f\u306a\u3089\u305a\u3001\u30de\u30a6\u30b9\u7531\u6765\u306e\u7d30\u80de\u682a\u306b\u304a\u3044\u3066\u3082\u3001\u4f8b\u3048\u3070NIH3T3\u7d30\u80de\u3068L929\u7d30\u80de\u3068\u306f\u3069\u3061\u3089\u3082\u6c4e\u7528\u6027\u306e\u9ad8\u3044\u4ed8\u7740\u6027\u7d30\u80de\u682a\u3067\u3042\u308b\u304c\u3001\u65e5\u672c\u306e\u7814\u7a76\u5ba4\u3067\u306f\u4e21\u7d30\u80de\u682a\u304c\u304a\u4e92\u3044\u306b\u6df7\u540c\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u304c\u591a\u3044\u3068\u63a8\u5bdf\u3055\u308c\u3066\u3044\u308b\u3002 \u5f93\u3063\u3066\u3001\u5404\u7814\u7a76\u5ba4\u306b\u306f\u3001\u72ec\u81ea\u306b\u9577\u671f\u9593\u4fdd\u5b58\u3057\u3066\u3044\u308b\u3088\u3046\u306a\u7d30\u80de\u682a\u304c\u591a\u6570\u3042\u308b\u3053\u3068\u3068\u63a8\u5bdf\u3059\u308b\u304c\u3001\u7814\u7a76\u76ee\u7684\u306b\u3088\u3063\u3066\u306f\u3001\u300c\u4ed6\u7d30\u80de\u306e\u6df7\u5165\u306b\u3088\u308b\u7f6e\u63db\u300d\u307e\u305f\u306f\u300c\u4ed6\u7d30\u80de\u3068\u306e\u53d6\u308a\u9055\u3048\u300d\u306e\u6709\u7121\u306b\u95a2\u3057\u3066\u306e\u691c\u67fb\u3092\u53b3\u5bc6\u306b\u5b9f\u65bd\u3059\u308b\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u308b\u3002<br \/>\n<strong>\u3000\u3000<\/strong>\u5f53\u5ba4\u4ee5\u5916\u306eATCC (American Type Culture Collection, U.S.A.)\u53ca\u3073JCRB (Japanese Cancer Research Resources Bank\u3001\u65e5\u672c)\u7b49\u306e\u7d30\u80de\u30d0\u30f3\u30af\u3067\u3082Multiplex STR-PCR\u89e3\u6790\u3092\u7d99\u7d9a\u7684\u306b\u5b9f\u65bd\u3057\u3066\u304a\u308a\u3001\u305d\u306e\u89e3\u6790\u7d50\u679c\u306f\u968f\u6642\u516c\u958b\u3057\u3001\u5404\u3005\u306e\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u304b\u3089\u5165\u624b\u53ef\u80fd\u3067\u3042\u308b\uff085\uff09\u3002 \u5404\u7d30\u80de\u30d0\u30f3\u30af\u3067\u306e\u89e3\u6790\u7d50\u679c\u3092\u6bd4\u8f03\u691c\u8a0e\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306a\u3088\u3046\u306b\u3001\u4e0a\u8a182\u7d30\u80de\u30d0\u30f3\u30af\u3068\u5f53\u5ba4\u3068\u306f\u3001\u540c\u3058Promega PowerPlex<sup>R<\/sup>1.2 \u3092\u7528\u3044\u3066\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u3066\u3044\u308b\u3002 \u5f93\u3063\u3066\u3001\u5404\u7814\u7a76\u5ba4\u306b\u304a\u3044\u3066\u306f\u3001Promega PowerPlex<sup>R<\/sup>1.2 \u3092\u7528\u3044\u3066\u72ec\u81ea\u306bMultiplex STR-PCR\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u3001\u305d\u306e\u7d50\u679c\u3092\u5404\u7d30\u80de\u30d0\u30f3\u30af\u304c\u6240\u6709\u3059\u308b\u7d30\u80de\u682a\u306e\u89e3\u6790\u7d50\u679c\u3068\u6bd4\u8f03\u691c\u8a0e\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\uff086\uff09\u3002 \u3057\u304b\u3057\u3001Multiplex STR-PCR\u5b9f\u9a13\u3092\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3057\u89e3\u6790\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u306f\u3001\u3069\u3053\u306e\u7814\u7a76\u5ba4\u3067\u3082\u8fc5\u901f\u306b\u5bb9\u6613\u306b\u5b9f\u65bd\u3067\u304d\u308b\u3088\u3046\u306a\u3082\u306e\u3067\u306f\u306a\u3044\u3053\u3068\u3082\u4e8b\u5b9f\u3067\u3042\u308b\u3002 \u305d\u3053\u3067\u3001\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u306f\u3001\u81ea\u3089\u306e\u7814\u7a76\u5ba4\u3067\u9577\u671f\u9593\u4fdd\u6709\u3059\u308b\u3053\u3068\u306f\u907f\u3051\u3001\u65b0\u898f\u7814\u7a76\u8ab2\u984c\u3092\u958b\u59cb\u3059\u308b\u524d\u306b\u3001Multiplex STR-PCR\u89e3\u6790\u6cd5\u306b\u3088\u308a\u8d77\u6e90\u304c\u78ba\u8a8d\u3055\u308c\u3001\u7d30\u80de\u7279\u6027\u7b49\u304c\u660e\u78ba\u306a\u7d30\u80de\u682a\u3092\u7d30\u80de\u30d0\u30f3\u30af\u304b\u3089\u5165\u624b\u3059\u308b\u3053\u3068\u304c\u3001\u975e\u5e38\u306b\u6709\u52b9\u306a\u5bfe\u5fdc\u7b56\u3067\u3042\u308b\u3068\u601d\u308f\u308c\u308b\u3002 \u307e\u305f\u3001\u6a39\u7acb\u3057\u3001\u7814\u7a76\u304c\u4e00\u6bb5\u843d\u3064\u3044\u305f\u3088\u3046\u306a\u7d30\u80de\u682a\u306b\u95a2\u3057\u3066\u306f\u3001\u7a4d\u6975\u7684\u306b\u7d30\u80de\u30d0\u30f3\u30af\u306b\u5bc4\u8a17\u3057\u3066\u9802\u304d\u3001\u7d30\u80de\u30d0\u30f3\u30af\u3067\u306e\u53b3\u5bc6\u306a\u54c1\u8cea\u7ba1\u7406\u306e\u4e0b\u3067\u3001\u4ed6\u306e\u7814\u7a76\u8005\u3078\u306e\u63d0\u4f9b\u3092\u884c\u3046\u3053\u3068\u3092\u63a8\u5968\u3059\u308b\u3002 \u3053\u3046\u3057\u305f\u7814\u7a76\u8005\u306e\u7406\u89e3\u3068\u5354\u529b\u306f\u3001\u5b9f\u9a13\u518d\u73fe\u6027\u304c\u78ba\u4fdd\u3055\u308c\u305f\u9ad8\u54c1\u8cea\u306a\u7d30\u80de\u6750\u6599\u3092\u7528\u3044\u305f\u7814\u7a76\u3092\u666e\u53ca\u3055\u305b\u3001\u751f\u547d\u79d1\u5b66\u5206\u91ce\u306e\u767a\u5c55\u306b\u5927\u304d\u306a\u8ca2\u732e\u3092\u3059\u308b\u3082\u306e\u3067\u3042\u308b\u3068\u8003\u3048\u3066\u3044\u308b\u3002<\/p>\n<p><a name=\"f\"><\/a><\/p>\n<hr \/>\n<h3>\u6587\u732e\u53ca\u3073\u53c2\u7167<\/h3>\n<p style=\"text-align: right;\"><a href=\"#top\">TOP\u25b2<\/a><\/p>\n<p>1)Gartler, S.M. Genetic markers as tracers in cell culture. Natl. Cancer Inst. Monogr. <strong>26<\/strong>: 167-195 (1967)<\/p>\n<p>2)Lins, A.M., Micka, K.A., Sprecher, C.J., Taylor, J.A., Bacher, J.W., Rabbach, D.R., Bever, R.A., Creacy, S.D., and Schumm, J.W. Development and population study of an eight-locus short tandem repeat (STR) multiplex system. J. Forensic Sci. <strong>43<\/strong>: 1168-1180 (1998)<\/p>\n<p>3)Masters, J.R., Thomson, J.A., Daly-Burns, B., Reid, Y.A., Dirks, W.G., Packer, P., Toji, L.H., Ohno, T., Tanabe, H., Arlett, C.F., Kelland, L.R., Harrison, M., Virmani, A., Ward, T.H., Ayres, K.L., and Debenham, P.G. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. U.S.A. <strong>98<\/strong>: 8012-8017 (2001)<\/p>\n<p>4)\u300c\u30b0\u30e9\u30d5\u30a3\u30ab\u30eb\u30e2\u30c7\u30ea\u30f3\u30b0\u306e\u5b9f\u969b\u300d\u65e5\u672c\u54c1\u8cea\u7ba1\u7406\u5b66\u4f1a\u7de8 (1999)<\/p>\n<p>5)\u672c\u5831\u544a\u66f8\u306b\u8a18\u8f09\u3057\u305f\u5f53\u30bb\u30f3\u30bf\u30fc\u306e\u89e3\u6790\u7d50\u679c\u306f\u8fd1\u65e5\u4e2d\u306b\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306b\u3066\u3082\u516c\u958b\u3059\u308b\u4e88\u5b9a\u3002<\/p>\n<p>6)\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u4e0a\u3067\u306e\u30b7\u30b9\u30c6\u30e0\u304c\u5b8c\u5099\u3067\u304d\u308b\u307e\u3067\u306e\u9593\u306f\u3001\u500b\u5225\u306e\u7d30\u80de\u682a\u306e\u7167\u4f1a\u306b\u5fdc\u3058\u307e\u3059\u3002 \u5373\u3061\u3001\u89e3\u6790\u7d50\u679c\u3092\u304a\u77e5\u3089\u305b\u9802\u3051\u308c\u3070\u3001\u305d\u306e\u7d50\u679c\u306f\u5f53\u30bb\u30f3\u30bf\u30fc\u304c\u4fdd\u6709\u3059\u308b\u3069\u306e\u7d30\u80de\u682a\u3068\u4e00\u81f4\u3059\u308b\u304b\u3001\u3042\u308b\u3044\u306f\u4e00\u81f4\u3059\u308b\u7d30\u80de\u682a\u306f\u306a\u3044\u7b49\u306e\u60c5\u5831\u3092\u8fd4\u4fe1\u81f4\u3057\u307e\u3059\u3002<\/p>\n<hr \/>\n","protected":false},"excerpt":{"rendered":"<p>\u623b\u308b &nbsp; \u00a0\u8981\u65e8 \u00a0\u306f\u3058\u3081\u306b \u00a0\u6750\u6599\u3068\u65b9\u6cd5 \u00a0\u7d50\u679c \u00a0\u8003\u5bdf \u00a0\u6587\u732e\u53ca\u3073\u53c2\u7167 \u4e2d\u6751\u5e78\u592b1\u3001\u5409\u91ce\u4f73\u7e541,\u3000\u5ca9\u702c\u3000\u79c02\u3001\u98ef\u6751\u6075\u7f8e1\u3001\u897f\u689d\u3000\u85ab1\u3001\u6df1\u6d77\u3000\u85ab2\u3001\u5927\u91ce\u5fe0\u592b1\u3001\u5c0f\u5e61\u88d5\u4e003 1\u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc\u7d30\u80de\u6750\u6599\u958b\u767a\u5ba4\u30012\u60c5\u5831\u89e3\u6790\u6280\u8853\u5ba4\u30013\u7406\u5316\u5b66\u7814\u7a76\u6240\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u30bb\u30f3\u30bf\u30fc \u8981\u65e8 TOP\u25b2 \u00a0\u00a0\u00a0\u7d30\u80de\u6750\u6599\u3092\u7528\u3044\u305f\u7814\u7a76\u6210\u679c\u304c\u518d\u73fe\u6027\u306e\u3042\u308b\u79d1\u5b66\u7684\u306a\u3082\u306e\u3067\u3042\u308b\u305f\u3081\u306b\u306f\u3001\u7528\u3044\u305f\u7d30\u80de\u6750\u6599\u306e\u8d77\u6e90 [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3256,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3715","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3715","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=3715"}],"version-history":[{"count":20,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3715\/revisions"}],"predecessor-version":[{"id":10477,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3715\/revisions\/10477"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3256"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=3715"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}