{"id":3560,"date":"2013-04-11T10:44:40","date_gmt":"2013-04-11T01:44:40","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3560"},"modified":"2017-07-12T14:55:24","modified_gmt":"2017-07-12T05:55:24","slug":"isozyme","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/quality\/isozyme","title":{"rendered":"Isozyme"},"content":{"rendered":"<p>\u7d30\u80de\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u691c\u67fb\u3067\u3059\u3002\u65ed\u785d\u5b50\u793e\u88fd\u306e\u30aa\u30fc\u30bb\u30f3\u30c6\u30a3\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\uff08\u73fe\u5728\u306f\u8ca9\u58f2\u4e2d\u6b62\uff09<br \/>\n\u7d30\u80de\u306e\u62bd\u51fa\u6db2\u3092\u96fb\u6c17\u6cf3\u52d5\u3057\u3001\u96fb\u6c17\u6cf3\u52d5\u5f8c\u306b\u76ee\u7684\u306e\u9175\u7d20\u3092\u9175\u7d20\u57fa\u8cea\u6db2\u3068\u53cd\u5fdc\u3055\u305b\u3066\u767a\u8272\u3055\u305b\u307e\u3059\u3002\u96fb\u6c17\u6cf3\u52d5\u306e\u79fb\u52d5\u5ea6\u304c\u52d5\u7269\u7a2e\u306b\u3088\u308a\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u3066\u3001\u7531\u6765\u52d5\u7269\u7a2e\u3092\u7279\u5b9a\u3057\u307e\u3059\u3002<br \/>\n\u9175\u7d20\u57fa\u8ceaLD\uff08Lactate Dehydrogenase\uff09\u3068NP\uff08Purine nucleoside\u3000phosphorylase\uff09\u3092\u30eb\u30fc\u30c1\u30f3\u306b\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n\u203b\u73fe\u5728\u306f\u3001Isozyme\u691c\u67fb\u306e\u4ee3\u308f\u308a\u306b\u52d5\u7269\u7a2ePCR\u691c\u67fb\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><strong>1. \u6e96\u5099\u3059\u308b\u3082\u306e<\/strong><\/p>\n<p>(1) \u691c\u4f53<\/p>\n<ul>\n<li>\u691c\u67fb\u691c\u4f53\uff08\u7d30\u80de\u30da\u30ec\u30c3\u30c8\uff09\n<li>\u967d\u6027\u5bfe\u71671\uff1aHeLa S-3 \u62bd\u51fa\u7269(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-039)\n<li>\u967d\u6027\u5bfe\u71672\uff1aL929 \u62bd\u51fa\u7269(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-037)\n<\/ul>\n<p>(2) \u8a66\u85ac<\/p>\n<ul>\n<li>Cell Extraction Buffer (\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, # 61-039-030)\n<li>Enzyme Stabilizer \uff08\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-032\uff09\n<li>\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30b2\u30eb\/\uff18(\u30d8\u30ec\u30ca\u7814\u7a76\u6240, #470100)\n<li>\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc(\u30d8\u30ec\u30ca\u7814\u7a76\u6240, #470180)\n<li>\u9175\u7d20\u57fa\u8cea LD(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-014)\n<li>\u9175\u7d20\u57fa\u8cea NP(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-002)\n<li>\u9175\u7d20\u57fa\u8cea PepB(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-010)\n<li>\u9175\u7d20\u57fa\u8cea AST(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-012)\n<li>\u9175\u7d20\u57fa\u8cea MPI(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-008)\n<li>\u9175\u7d20\u57fa\u8cea G6PD(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\u793e, #61-039-004)\n<li>\u9175\u7d20\u5b89\u5b9a\u7528\u30d0\u30c3\u30d5\u30a1\u30fc(\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9 \u30b3\u30fc\u30c9\u756a\u53f7\uff1aR-578)\n<\/ul>\n<p>(3) \u5bb9\u5668<\/p>\n<ul>\n<li>\u30aa\u30fc\u30bb\u30f3\u30c6\u30a3\u30ad\u30c3\u30c8\u5c02\u7528\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e\uff08\u65ed\u30c6\u30af\u30ce\u30b0\u30e9\u30b9\uff09\n<li>\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\n<li>\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30c8\u30ec\u30a4\n<\/ul>\n<p><strong>2. \u65b9\u6cd5<\/strong><\/p>\n<p>(1) \u691c\u4f53\u306e\u8abf\u88fd<br \/>\n\u3000\u691c\u4f53\u306e\u8abf\u88fd\u306f\u6c37\u4e0a\u3067\u884c\u3046\u3002<\/p>\n<ol class=\"list_parentheses\">\n<li>\u7d30\u80de(\u7d042\u00d7106\u500b)\u3092\u56de\u53ce\u3059\u308b(\u30da\u30ec\u30c3\u30c8\u306e\u72b6\u614b\u306750\u03bcl\u4ee5\u4e0a\u304c\u671b\u307e\u3057\u3044)\u3002\n<li>PBS(-)\u30672\u56de\u9060\u5fc3\u6d17\u6d44\u3057\u305f\u5f8c\u3001\u6d17\u6d44\u6db2\u3092\u6368\u3066\u308b\u3002\uff08\u7d30\u80de\u30da\u30ec\u30c3\u30c8\uff09\n<li>\u7d30\u80de\u30da\u30ec\u30c3\u30c8\u3068\u7b49\u91cf\u306eCell Extraction Buffer(10\uff5e100\u03bcl\u7a0b\u5ea6)\u3092\u52a0\u3048\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u3088\u308a\u30da\u30ec\u30c3\u30c8\u3092\u5b8c\u5168\u306b\u61f8\u6fc1\u3059\u308b\u3002\n<li>15\u5206\u9593\u6c37\u4e0a\u306b\u9759\u7f6e\u3059\u308b\u3002\n<li>\u30df\u30ad\u30b5\u30fc\u306b2\u30013\u56de\u8efd\u304f\u62bc\u3057\u5f53\u3066\u308b\u3088\u3046\u306b\u3057\u3066\u652a\u62cc\u3059\u308b\u3002\n<li>15000rpm\u30014\u2103\u30015\u5206\u9593\u9060\u5fc3\u3059\u308b\u3002\n<li>\u4e0a\u6e05\u3092\u65b0\u3057\u3044\u5fae\u91cf\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u306b\u79fb\u3059\u3002\u3053\u308c\u3092\u6cf3\u52d5\u7528\u30b5\u30f3\u30d7\u30eb\u3068\u3059\u308b\u3002\n<li>\u51cd\u7d50\u4fdd\u5b58\u3059\u308b\u5834\u5408\u306f\u3001\u4e0a\u6e05\u3068\u540c\u91cf\u306e\u9175\u7d20\u5b89\u5b9a\u7528\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u52a0\u3048\u3001-20\u2103\u306b\u51cd\u7d50\u4fdd\u5b58\u3059\u308b\u3002\n<\/ol>\n<p>(2) \u96fb\u6c17\u6cf3\u52d5<\/p>\n<ol class=\"list_parentheses\">\n<li>\u5c02\u7528\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e\u306e\u30ab\u30d0\u30fc\u3092\u6c37\u6c34\u3067\u6e80\u305f\u3059\u3002\n<li>\u30b2\u30eb\u306e\u30a6\u30a7\u30eb\u306b\u3001\u30b5\u30f3\u30d7\u30eb\u3068\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u306a\u308bL929 \u62bd\u51fa\u7269\u3001HeLa S-3 \u62bd\u51fa\u7269\u30920.8\u03bcl\u305a\u3064\u30a2\u30d7\u30e9\u30a4\u3059\u308b\u3002\n<li>4\u2103\u306b\u51b7\u5374\u3057\u305f\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u5c02\u7528\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e\u306b\u5165\u308c\u3001\u30b2\u30eb\u3092\u30bb\u30c3\u30c8\u3059\u308b\u3002\n<li>90V\u300160\u5206\u9593\u96fb\u6c17\u6cf3\u52d5\u3059\u308b\u3002\n<\/ol>\n<p>(3) \u67d3\u8272<\/p>\n<ol class=\"list_parentheses\">\n<li>\u9175\u7d20\u57fa\u8cea\u3092500\u03bcl\u306e\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6eb6\u89e3\u3059\u308b\u3002\n<li>\u5fae\u91cf\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u306b\u79fb\u3057\u3001\u8efd\u304f\u9060\u5fc3\u3057\u3066\u4e0d\u6eb6\u7269\u3092\u6c88\u964d\u3055\u305b\u308b\u3002\n<li>\u6cf3\u52d5\u5f8c\u306e\u30b2\u30eb\u3092\u6cf3\u52d5\u69fd\u30ab\u30d0\u30fc\u304b\u3089\u306f\u305a\u3059\u3002\n<li>\u30b2\u30eb\u306e\uff0b\u6975\u5074\u306b\u6eb6\u89e3\u3057\u305f\u9175\u7d20\u57fa\u8cea\u3092\u306e\u305b\u308b\u3002\n<li>\u9175\u7d20\u57fa\u8cea\u6db2\u3092\u30b2\u30eb\u4e0a\u306b\u5747\u4e00\u306b\u5857\u308a\u5e83\u3052\u308b\u3002\n<li>\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30c8\u30ec\u30a4\u306b\u8d85\u7d14\u6c34\u3067\u6e7f\u3089\u305b\u305f\u30ad\u30e0\u30ef\u30a4\u30d7\u3092\u6577\u304d\u3001\u305d\u306e\u4e0a\u306b\u30b2\u30eb\u3092\u4e57\u305b\u3066\u84cb\u3092\u3059\u308b\u3002\n<li>37\u2103\u3067\u767a\u8272\u306e\u69d8\u5b50\u3092\u89b3\u5bdf\u3057\u306a\u304c\u3089\u300110\uff5e30\u5206\u9593\u7a0b\u5ea6\u53cd\u5fdc\u3055\u305b\u308b\u3002\n<li>\u30d0\u30f3\u30c9\u304c\u73fe\u308c\u305f\u3089\u3001\u8d85\u7d14\u6c34\u3092\u5165\u308c\u305f\u30d0\u30c3\u30c8\u306b\u30b2\u30eb\u3092\u6d78\u3057\u306615\u5206\u9593\u7a0b\u5ea6\u632f\u76ea\u6a5f\u3067\u8efd\u304f\u632f\u308a\u3001\u4f59\u5206\u306a\u9175\u7d20\u3092\u9664\u304f\u3002\n<li>\u8d85\u7d14\u6c34\u3092\u65b0\u3057\u3044\u3082\u306e\u306b\u4ea4\u63db\u3057\u3001\u3055\u3089\u306b15\u5206\u9593\u7a0b\u5ea6\u632f\u76ea\u3059\u308b\u3002\n<li>\u30b2\u30eb\u3092\u4e00\u6669\u5ba4\u6e29\u3067\u4e7e\u71e5\u3055\u305b\u308b\u3002\n<\/ol>\n<p>\u7d50\u679c\u4f8b<\/p>\n<table>\n<tbody>\n<tr>\n<td id=\"t1\" style=\"text-align: center;\">\u9175\u7d20\u57fa\u8ceaLD<\/td>\n<td id=\"t1\" style=\"text-align: center;\">\u9175\u7d20\u57fa\u8ceaNP<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/isozyme_LD.jpg\"><\/a><\/td>\n<td id=\"t1\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/isozyme_NP.jpg\"><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>1. HeLa.S3\u62bd\u51fa\u7269 (\u30d2\u30c8)\uff08\u967d\u6027\u5bfe\u71671\uff09\u30002. \u30d2\u30c8\u30003. \u30a6\u30b5\u30ae\u30004. \u30df\u30f3\u30af\u30005. \u30df\u30f3\u30af\u30006. \u30de\u30a6\u30b9\u30007. \u30de\u30a6\u30b9\u30008. L929\u62bd\u51fa\u7269\uff08\u30de\u30a6\u30b9\uff09\uff08\u967d\u6027\u5bfe\u71672\uff09<\/p>\n<p><strong>3. \u5224\u5b9a<\/strong><\/p>\n<p>\u5404\u30ec\u30fc\u30f3\u306e\u30d0\u30f3\u30c9\u304c\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u306a\u308bL929\uff08Mouse\uff09\u53ca\u3073HeLa S3\uff08Human\uff09\u306e\u30d0\u30f3\u30c9\u4f4d\u7f6e\u3068\u6bd4\u8f03\u3057\u3066\u76f8\u5bfe\u7684\u306b\u6b63\u3057\u3044\u4f4d\u7f6e\u306b\u51fa\u3066\u3044\u308b\u304b\u3001\u30d0\u30f3\u30c9\u30d1\u30bf\u30fc\u30f3\u56f3\u3068\u7167\u5408\u3057\u78ba\u8a8d\u3059\u308b\u3002<\/p>\n<p>\u52d5\u7269\u7a2e\u306b\u3088\u308b\u79fb\u52d5\u5ea6\uff08\u30d0\u30f3\u30c9\u30d1\u30bf\u30fc\u30f3\u56f3\uff09\u4f8b<br \/>\n<img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/isozyme_img3.jpg\"><\/a><\/p>\n<p>\u30fb\u30d0\u30f3\u30c9\u30d1\u30bf\u30fc\u30f3\u56f3\u6587\u732e<br \/>\nThe AuthentiKit System Handbook For Cell Authentication And Identification (Innovative Chemistry)<br \/>\nJCRB NEWSLETTER\u5225\u518ap30 1985 Oct<br \/>\nIn vitro 16(2) 119-135 1980 PubMed ID : 6988327<\/p>\n<p>\u30fb\u30d0\u30f3\u30c9\u30d1\u30bf\u30fc\u30f3\u56f3\u306b\u8a18\u8f09\u306e\u52d5\u7269\u7a2e<br \/>\n\u9175\u7d20\u57fa\u8ceaLD\u3001NP\u3001G6PD\u3001PepB\u3001MPI\u3001AST\uff1ahuman\u3001mouse\u3001rat\u3001dog\u3001monkey(cercopithecus)\u3001monkey(rhesus)\u3001fox\u3001horse\u3001cat\u3001guinea pig\u3001hamster(syrian)\u3001hamster(chinese)\u3001bovine\u3001rabbit\u3001aoudad\u3001raccoon\u3001baboon\u3001bat\u3001buffalo\u3001mink<br \/>\n\u9175\u7d20\u57fa\u8ceaLD\u3001NP\u3001G6PD\uff1acattle<br \/>\n\u9175\u7d20\u57fa\u8ceaNP\u3001PepB\u3001MPI\uff1amarmoset\u3001goat\u3001pig\uff08G6PD\u3082\u53ef\uff09\u3001sheep<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d30\u80de\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u691c\u67fb\u3067\u3059\u3002\u65ed\u785d\u5b50\u793e\u88fd\u306e\u30aa\u30fc\u30bb\u30f3\u30c6\u30a3\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\uff08\u73fe\u5728\u306f\u8ca9\u58f2\u4e2d\u6b62\uff09 \u7d30\u80de\u306e\u62bd\u51fa\u6db2\u3092\u96fb\u6c17\u6cf3\u52d5\u3057\u3001\u96fb\u6c17\u6cf3\u52d5\u5f8c\u306b\u76ee\u7684\u306e\u9175\u7d20\u3092\u9175\u7d20\u57fa\u8cea\u6db2\u3068\u53cd\u5fdc\u3055\u305b\u3066\u767a\u8272\u3055\u305b\u307e\u3059\u3002\u96fb\u6c17\u6cf3\u52d5\u306e\u79fb\u52d5\u5ea6\u304c\u52d5\u7269\u7a2e\u306b\u3088\u308a\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u3066\u3001\u7531\u6765\u52d5\u7269\u7a2e\u3092\u7279\u5b9a\u3057\u307e\u3059\u3002 \u9175\u7d20\u57fa\u8ceaLD\uff08Lactate Dehydrogenase\uff09\u3068NP\uff08Purine nucleoside\u3000phosphorylase\uff09\u3092\u30eb\u30fc [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3425,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3560","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3560","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=3560"}],"version-history":[{"count":13,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3560\/revisions"}],"predecessor-version":[{"id":10490,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3560\/revisions\/10490"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3425"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=3560"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}