{"id":3498,"date":"2013-04-10T17:17:02","date_gmt":"2013-04-10T08:17:02","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3498"},"modified":"2025-01-30T16:24:07","modified_gmt":"2025-01-30T07:24:07","slug":"myco_kensa","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/quality\/myco_kensa","title":{"rendered":"\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u691c\u67fb"},"content":{"rendered":"

\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306f\u5fae\u5c0f\u306a\u751f\u7269\u3067\u3042\u308b\u305f\u3081\u3001\u57f9\u990a\u7d30\u80de\u3084\u57f9\u990a\u6db2\u3092\u6c5a\u67d3\u3057\u3066\u3044\u3066\u3082\u6c17\u4ed8\u304b\u306a\u3044\u3053\u3068\u304c\u307b\u3068\u3093\u3069\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u6c5a\u67d3\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u5185\u306e\u307b\u3068\u3093\u3069\u3059\u3079\u3066\u306e\u4ee3\u8b1d\u53cd\u5fdc\u3001\u7d30\u80de\u306e\u7279\u6027\u3084\u5897\u6b96\u306b\u307e\u3067\u5f71\u97ff\u3092\u53ca\u307c\u3059\u3068\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u6c17\u4ed8\u304b\u306a\u3044\u3046\u3061\u306b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u6c5a\u67d3\u304c\u7d30\u80de\u57f9\u990a\u74b0\u5883\u306b\u5e83\u304c\u3063\u3066\u3044\u308b\u53ef\u80fd\u6027\u3082\u3042\u308a\u3001\u65e5\u5e38\u7684\u306b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u691c\u67fb\u3092\u884c\u3046\u3053\u3068\u304c\u671b\u307e\u308c\u307e\u3059\u3002<\/p>\n

\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u691c\u51fa\u6cd5\u306b\u306f\u6570\u591a\u304f\u306e\u65b9\u6cd5\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u5f53\u5ba4\u3067\u306f\u3001\u86cd\u5149\u8272\u7d20\u306b\u3088\u308b\u691c\u51fa\u65b9\u6cd5\u3068Nested-PCR\u306b\u3088\u308b\u691c\u51fa\u65b9\u6cd5\u3092\u4f75\u7528\u3057\u3066\u3001\u7dcf\u5408\u7684\u306b\u5224\u5b9a\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u30fb\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u691c\u67fb\u306e\u524d\u306b\u30fb\u30fb\u30fb<\/strong>
\n\u6297\u751f\u7269\u8cea\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u304c\u691c\u51fa\u3055\u308c\u3065\u3089\u304f\u3001\u3088\u308a\u6b63\u78ba\u306b\u9ad8\u611f\u5ea6\u306b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u6c5a\u67d3\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u3001\u6297\u751f\u7269\u8cea\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u30673\u9031\u9593\u4ee5\u4e0a\u57f9\u990a\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

\u86cd\u5149\u8272\u7d20\u306b\u3088\u308b\u691c\u51fa\u65b9\u6cd5<\/a>\u3000\u203b \u53c2\u8003\u6587\u732e (1)\uff5e(4)<\/p>\n

\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u6838DNA\u3092\u86cd\u5149\u8272\u7d20\uff08Hoechst 33258\uff09\u3067\u67d3\u8272\u3057\u86cd\u5149\u9855\u5fae\u93e1\u3067\u76f4\u63a5\u89b3\u5bdf\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u57f9\u990a\u7d30\u80de\u306e\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u6c5a\u67d3\u3092\u691c\u51fa\u3057\u307e\u3059\u3002\u6307\u6a19\u7d30\u80de\u3068\u3057\u3066Vero\u7d30\u80de\u3092\u7528\u3044\u3066\u30b5\u30f3\u30d7\u30eb\u3092\u5171\u57f9\u990a\u3057\u3001\u751f\u5b58\u3057\u3066\u3044\u308b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u5897\u6b96\u3092\u4fc3\u3059\u3053\u3068\u3067\u3001\u9ad8\u611f\u5ea6\u3067\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u3092\u5897\u6b96\u3055\u305b\u308b\u5fc5\u8981\u304c\u3042\u308b\u305f\u3081\u3001\u5224\u5b9a\u307e\u3067\u306b5\uff0d6\u65e5\u307b\u3069\u671f\u9593\u304c\u5fc5\u8981\u3067\u3042\u308a\u3001\u307e\u305f\u3001\u30b5\u30f3\u30d7\u30eb\u304a\u3088\u3073\u6307\u6a19\u7d30\u80de\u306e\u6b7b\u7d30\u80de\u306b\u7531\u6765\u3059\u308b\u6838\u65ad\u7247\u7b49\u3082\u67d3\u8272\u3055\u308c\u308b\u305f\u3081\u3001\u5224\u5b9a\u306b\u306f\u7d4c\u9a13\u3092\u8981\u3057\u307e\u3059\u3002
\n\u203b\u7814\u7a76\u7528\u30d2\u30c8\u81cd\u5e2f\u8840\u6750\u6599\uff08HCB , CBF , C34\uff09\u306f\u691c\u67fb\u3092\u884c\u3063\u3066\u304a\u308a\u307e\u305b\u3093\u3002<\/p>\n

\"\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306b\u6c5a\u67d3\u3055\u308c\u305fVero\u7d30\u80de\u306e\u86cd\u5149\u67d3\u8272\u753b\u50cf\"<\/a>
\n\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306b\u6c5a\u67d3\u3055\u308c\u305fVero\u7d30\u80de\u306e\u86cd\u5149\u67d3\u8272\u753b\u50cf
\uff08\u86cd\u5149\u8272\u7d20\uff1aHoechst 33258\u3001\u5bfe\u8c61\uff1a\u6838DNA\uff09<\/p>\n

Nested-PCR\u306b\u3088\u308b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u691c\u51fa\u65b9\u6cd5<\/a> \u203b\u53c2\u8003\u6587\u732e (5)\uff5e(8)
\n\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306f\u591a\u304f\u306e\u7a2e\u985e\u304c\u5b58\u5728\u3057\u3001\u4e0b\u56f3\u306e\u69d8\u306b\u7a2e\u306b\u3088\u3063\u306616S-23S rRNA\u306e\u30b9\u30da\u30fc\u30b5\u30fc\u9818\u57df\u306b\u591a\u578b\u3092\u793a\u3057\u307e\u3059\u3002\u3053\u306e\u591a\u578b\u3092\u631f\u3080\u5171\u901a\u914d\u5217\u3092\u6a19\u7684\u3068\u3057\u305fprimer\u3092\u7528\u3044\u3066\u3001Nested-PCR\u6cd5\u306b\u3088\u308a\u691c\u51fa\u3092\u884c\u3046\u305f\u3081\u3001\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u6709\u7121\u306e\u4ed6\u3001\u5897\u5e45\u30b5\u30a4\u30ba\u304b\u3089\u3042\u308b\u7a0b\u5ea6\u306e\u7a2e\u3092\u4e88\u6e2c\u3059\u308b\u4e8b\u3082\u3067\u304d\u307e\u3059\u3002\u5fae\u91cf\u306e\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306b\u3088\u308b\u6c5a\u67d3\u3092\u77ed\u6642\u9593\u3067\u7c21\u4fbf\u306b\u691c\u51fa\u30fb\u5224\u5b9a\u3067\u304d\u307e\u3059\u304c\u3001\u3059\u3079\u3066\u306e\u7a2e\u985e\u306e\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u3092\u691c\u51fa\u3067\u304d\u308b\u308f\u3051\u3067\u306f\u306a\u3044\u3068\u3044\u3046\u554f\u984c\u70b9\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

<\/a><\/p>\n

\u691c\u51fa\u3092\u78ba\u8a8d\u3057\u305f\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\u3068\u5897\u5e45\u30b5\u30a4\u30ba<\/p>\n

\u3053\u306ePCR\u3067\u691c\u51fa\u53ef\u80fd\u306a100\u6570\u5341\u7a2e\u985e\u306e\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de \u304a\u3088\u3073 \u8fd1\u7e01\u7a2e\u306e\u3046\u3061\u3001\u5f53\u5ba4\u3067\u5b9f\u969b\u306b\u691c\u51fa\u3092\u78ba\u8a8d\u3057\u3066\u3044\u308b\u7a2e\u306f12\u7a2e\u985e\u3002
\n\uff08* \u3000<\/span> \u306f\u57f9\u990a\u7d30\u80de\u306b\u611f\u67d3\u4f8b\u306e\u591a\u3044\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u7a2e)<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Species<\/td>\nMain host<\/td>\nStrains<\/td>\nSource [Cat #]<\/td>\n1st-step PCR product (bp)<\/td>\n2nd-step PCR product (bp)<\/td>\n
Mycoplasmopsis arginini<\/em><\/td>\nBovine<\/td>\nNCTC10129<\/td>\nMinerva Biolabs [51-0129]<\/td>\n370<\/td>\n236<\/td>\n<\/tr>\n
Mesomycoplasma hyorhinis<\/em><\/td>\nPorcine<\/td>\nNCTC10130<\/td>\nMinerva Biolabs [51-0130]<\/td>\n448<\/td>\n314<\/td>\n<\/tr>\n
Mycoplasmopsis fermentans<\/em><\/td>\nHuman<\/td>\nNCTC10117<\/td>\nMinerva Biolabs [51-0117]<\/td>\n492<\/td>\n366<\/td>\n<\/tr>\n
Metamycoplasma orale<\/em><\/td>\nHuman<\/td>\nNCTC10112<\/td>\nMinerva Biolabs [51-0112]<\/td>\n424<\/td>\n290<\/td>\n<\/tr>\n
Metamycoplasma hominis<\/em><\/td>\nHuman<\/td>\nATCC 23114<\/td>\nMinerva Biolabs [51-0111]<\/td>\n370<\/td>\n236<\/td>\n<\/tr>\n
Mycoplasmoides genitalium<\/em><\/td>\nHuman<\/td>\nATCC 33530<\/td>\nMinerva Biolabs [51-0195]<\/td>\n390<\/td>\n252<\/td>\n<\/tr>\n
Metamycoplasma salivarium<\/em><\/td>\nHuman<\/td>\nNCTC10113<\/td>\nMinerva Biolabs [51-0113]<\/td>\n403<\/td>\n269<\/td>\n<\/tr>\n
Mycoplasmoides pirum<\/em><\/td>\nHuman<\/td>\nATCC 25960<\/td>\nATCC [25960 D]<\/td>\n459<\/td>\n323<\/td>\n<\/tr>\n
Mycoplasmoides pneumoniae<\/em><\/td>\nHuman<\/td>\nNCTC10119<\/td>\nMinerva Biolabs [51-0119]<\/td>\n416<\/td>\n280<\/td>\n<\/tr>\n
Mycoplasmopsis synoviae<\/em><\/td>\nAvian<\/td>\nNCTC10124<\/td>\nMinerva Biolabs [51-0124]<\/td>\n496<\/td>\n362<\/td>\n<\/tr>\n
Mycoplasmoides gallisepticum<\/em><\/td>\nAvian<\/td>\nNCTC10115<\/td>\nMinerva Biolabs [51-0115]<\/td>\n846<\/td>\n710<\/td>\n<\/tr>\n
Spiroplasma citri<\/em><\/td>\nInsect, Plant<\/td>\nATCC 27556<\/td>\nMinerva Biolabs [51-0164]<\/td>\n462<\/td>\n326<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\u306e\u691c\u51fa\u611f\u5ea6\uff08\u30d0\u30f3\u30c9\u691c\u51fa\u56de\u6570 \/ PCR\u691c\u67fb\u56de\u6570\uff09<\/p>\n

<\/a><\/p>\n

Nested-PCR\u306b\u3088\u308bA. laidlawii<\/em> \u691c\u51fa\u65b9\u6cd5<\/a>\u3000\u203b\u53c2\u8003\u6587\u732e (9)<\/p>\n

\u57f9\u990a\u7d30\u80de\u306b\u611f\u67d3\u4f8b\u306e\u591a\u3044Acholeplasma<\/em>\u5c5e\u306e\u4e00\u7a2e\u300cAcholeplasma laidlawii<\/em>\u300d\u306b\u7279\u7570\u7684\u306aPrimer\u3092\u7528\u3044\u300116S-23S rRNA\u306e\u30b9\u30da\u30fc\u30b5\u30fc\u9818\u57df\u3092Nested-PCR\u6cd5\u306b\u3088\u3063\u3066\u691c\u51fa\u3057\u307e\u3059\u3002
\n<\/a><\/p>\n

\u691c\u51fa\u3092\u78ba\u8a8d\u3057\u305f\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\u3068\u5897\u5e45\u30b5\u30a4\u30ba<\/p>\n\n\n\n\n
Species<\/td>\nMain host<\/td>\nStrains<\/td>\nSource [Cat #]<\/td>\n1st-step PCR product (bp)<\/td>\n2nd-step PCR product (bp)<\/td>\n
Acholeplasma laidlawii<\/em><\/td>\nBovine<\/td>\nNCTC10116<\/td>\nMinerva Biolabs [51-0116]<\/td>\n392<\/td>\n249<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\u306e\u691c\u51fa\u611f\u5ea6\uff08\u30d0\u30f3\u30c9\u691c\u51fa\u56de\u6570 \/ PCR\u691c\u67fb\u56de\u6570\uff09
\n<\/a><\/p>\n

\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\u691c\u51fa\u30a4\u30e1\u30fc\u30b8
\n\u6dfb\u52a0\u3055\u308c\u305f\u92f3\u578b\u91cf\u306b\u3088\u3063\u3066\u30011st-step\u30682nd-step \u4e21\u65b9\u306e\u30d0\u30f3\u30c9\u304c\u73fe\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002
\n<\/a><\/p>\n

<\u53c2\u8003\u6587\u732e><\/p>\n

    \n
  1. IFO Res. Comm., 13, 52-58 (1987)\n
  2. Bull. Jap. Fed. Culture Collections, 4, 9-15 (1988)\n
  3. \u65e5\u672c\u7d44\u7e54\u57f9\u990a\u5b66\u4f1a\u7d30\u80de\u30d0\u30f3\u30af\u59d4\u54e1\u4f1a\u5831\u544a\u66f8\uff08\u5e73\u62102\u5e743\u6708\uff09\n
  4. \u65e5\u672c\u85ac\u5c40\u65b9\u3000\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u5fdc\u7528\u533b\u85ac\u54c1\/\u751f\u7269\u671f\u9650\u7531\u6765\u533b\u85ac\u54c1\u306e\u88fd\u9020\u306b\u7528\u3044\u308b\u7d30\u80de\u57fa\u6750\u306b\u5bfe\u3059\u308b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u5426\u5b9a\u8a66\u9a13\uff08\u7b2c17\u6539\u8a02\u3000\u53c2\u8003\u60c5\u5831\u3000\u5e73\u621028\u5e743\u6708\uff09\n
  5. Res. Microbiol., 144, 489-493 (1993)\n
  6. Rapid Diagnosis of Mycoplasmas,227-232[eds.I.Kahane&A.Adoni](1993)\n
  7. \u86cb\u767d\u8cea\u6838\u9178\u9175\u7d2040(15):2361-2368(1995)\n
  8. Mol & Diag. Proced. in\u3000Mycoplasmology,vol.\u2161A7[eds.Razin&Tully](1996)\n
  9. \u65e5\u672c\u5de5\u696d\u898f\u683cJIS K 3810-3:2003\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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