{"id":3406,"date":"2013-04-10T13:23:23","date_gmt":"2013-04-10T04:23:23","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3406"},"modified":"2026-08-03T10:18:24","modified_gmt":"2026-08-03T01:18:24","slug":"myco_keikou","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/quality\/myco_kensa\/myco_keikou","title":{"rendered":"\u86cd\u5149\u8272\u7d20\u306b\u3088\u308b\u691c\u51fa\u65b9\u6cd5"},"content":{"rendered":"<p>\u203b\u65e5\u672c\u7d44\u7e54\u57f9\u990a\u5b66\u4f1a\u306e\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\uff0d\u30eb\u306b\u6e96\u3058\u3066\u4e00\u90e8\u6539\u5909<\/p>\n<p><strong>1. \u6e96\u5099\u3059\u308b\u3082\u306e<\/strong><\/p>\n<ul>\n<li>\u691c\u4f53<\/li>\n<table>\n<tbody>\n<tr>\n<td id=\"t1\"><strong>\u8abf\u6574\u6cd5\u3000\uff1a<\/strong><\/td>\n<td id=\"t1\"><strong><span style=\"color: #ff0000;\">\u6297\u751f\u7269\u8cea\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u30673\u9031\u9593\u4ee5\u4e0a\u57f9\u990a<\/span>\u3057\u305f\u7d30\u80de\u3092\u6e96\u5099\u3057\u3001<span style=\"color: #ff0000;\">\u7d99\u4ee3\u5f8c\u307e\u305f\u306f\u57f9\u5730\u4ea4\u63db\u5f8c3\u65e5\u4ee5\u4e0a\u7d4c\u904e\u3057\u305f\u57f9\u990a\u4e0a\u6e05<\/span>\u3092\u63a1\u53d6\u3059\u308b<\/strong><br \/>\u203b\u6bce\u65e5\u57f9\u5730\u4ea4\u63db\u3092\u5fc5\u8981\u3068\u3059\u308b\u7d30\u80de\u306e\u5834\u5408\u306f\u7d30\u80de\u304c\u5341\u5206\u306b\u5897\u6b96\u3057\u305f\u30bf\u30a4\u30df\u30f3\u30b0\u3067\u57f9\u5730\u4ea4\u63db\u76f4\u524d\u306e\u57f9\u990a\u4e0a\u6e05\u3092\u7528\u3044\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/ul>\n<ul>\n<li>Vero\u7d30\u80de\u3000\uff08\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306b\u611f\u67d3\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u6e08\u306e\u3082\u306e\uff09<\/li>\n<p>\u7d30\u80de\u6fc3\u5ea6\u3000\uff1a\u30001-2 x 10<sup>4<\/sup>cells \/ 35dish (0.5mL)<br \/>\n\u5fc5\u8981\u306adish\u6570\u3000\uff1a\u3000\u691c\u4f53\u306e\u6570\u3000\uff0b\u3000Negative control\u7528 2\u679a\n<\/ul>\n<ul>\n<li>Vero\u7d30\u80de\u7528\u57f9\u5730<\/li>\n<div id=\"b1\">\u7d44\u6210\u3000\uff1aMEM+10%FBS\u3000(\u7528\u6642\u8abf\u88fd)\n<\/div>\n<\/li>\n<\/ul>\n<ul>\n<li>\u67d3\u8272\u539f\u6db2 \uff08-20\u2103\u4ee5\u4e0b\u3067\u4fdd\u5b58\uff09<\/li>\n<p>\u5ba4\u6e29\u306730-40\u5206\u9593\u3001\u30b9\u30bf\u30fc\u30e9\u30fc\u3092\u4f7f\u3063\u3066\u3088\u304f\u652a\u62cc\u3057\u30011\u56de\u306b\u4f7f\u3046\u5fc5\u8981\u91cf\u3092\u5206\u6ce8\u3057\u3001\u30a2\u30eb\u30df\u30db\u30a4\u30eb\u3067\u5b8c\u5168\u306b\u5305\u307f\u906e\u5149\u3059\u308b<\/p>\n<div id=\"b1\">\u7d44\u6210\u3000\uff1a5.0mg\u3000Hoechst 33258 (American Hoechst, Co., 33217)<br \/>\n100mL\u3000PBS(\uff0d)\n<\/div>\n<\/ul>\n<ul>\n<li>Mounting solution\uff08PH 5.5\u306b\u8abf\u6574\u30014\u2103 \u4fdd\u5b58\uff09<\/li>\n<div id=\"b1\">\u7d44\u6210\u3000\uff1a22.2mL\u30000.1M \u30af\u30a8\u30f3\u9178 (MW 210.14, 2.101g\/100mL)<br \/>\n27.8mL\u30000.2M Na<sub>2<\/sub>HPO<sub>4<\/sub>\u30fb12H<sub>2<\/sub>O (MW 358.14, 7.162g\/100mL)<br \/>\n50mL\u3000\u30b0\u30ea\u30bb\u30ed\u30fc\u30eb\n<\/div>\n<\/ul>\n<ul>\n<li>\u56fa\u5b9a\u6db2<\/li>\n<div id=\"b1\">\u7d44\u6210\u3000\uff1a99\uff05 \u30a8\u30bf\u30ce\u30fc\u30eb : \u9162\u9178 = 3 : 1 \u3000(\u7528\u6642\u8abf\u88fd\u3001\u6c37\u51b7)\n<\/div>\n<\/ul>\n<ul>\n<li>PBS(\uff0d)\u5e0c\u91c8\u56fa\u5b9a\u6db2<\/li>\n<div id=\"b1\">\u7d44\u6210\u3000\uff1aPBS(\uff0d) : \u56fa\u5b9a\u6db2 = 3 : 1\u3000 (\u7528\u6642\u8abf\u88fd)\n<\/div>\n<\/ul>\n<ul>\n<li>\u84b8\u7559\u6c34 <\/li>\n<li>35mm dish <\/li>\n<li>\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u300022 x 22 \uff08mm\uff09\u3000\uff08\u4e7e\u71e5\u6ec5\u83cc\u6e08\u307f\uff09 <\/li>\n<li>\u30d4\u30f3\u30bb\u30c3\u30c8 <\/li>\n<li>\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9 <\/li>\n<li>\u86cd\u5149\u9855\u5fae\u93e1 <\/li>\n<\/ul>\n<p><strong>2. \u65b9\u6cd5<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td id=\"t1\"><strong>\u6ce8\u610f\u3000<\/strong><\/td>\n<td id=\"t1\"><strong>(1) \u2013 (6) \u306e\u64cd\u4f5c\u306f\u7121\u83cc\u64cd\u4f5c\u3092\u884c\u3046\u3002<br \/>\n(7) \u2013 (17) \u306e\u64cd\u4f5c\u306f\u7121\u83cc\u64cd\u4f5c\u4e0d\u8981\u3002<br \/>\n(8) \u2013 (14) \u306e\u64cd\u4f5c\u9593\u306f\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u4e7e\u71e5\u3055\u305b\u306a\u3044\u3002<br \/>\n\u7279\u306b(10) (11)\u306e\u5f8c\u3067\u6025\u6fc0\u306a\u4e7e\u71e5\u304c\u8d77\u3053\u308b\u3068\u7d30\u80de\u306e\u5f62\u614b\u304c\u5d29\u308c\u3084\u3059\u304f\u306a\u308b\u306e\u3067\u6ce8\u610f\u3002<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table width=\"650px\">\n<tbody>\n<tr>\n<td id=\"t1\" valign=\"top\">(1)<\/td>\n<td id=\"t1\">\u4e7e\u71b1\u6ec5\u83cc\u3057\u305f22 x 22 \uff08mm\uff09\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u66f4\u306b\u706b\u708e\u6ec5\u83cc\u3057\u300135mm dish\u306b\u5165\u308c\u308b \uff08\u56f31\uff09<\/td>\n<td id=\"t1\" rowspan=\"5\"><a href=\"\/ja\/wp-content\/uploads\/images\/myco_dna_dish_1.png\"><img decoding=\"async\" class=\"gazou\" src=\"\/ja\/wp-content\/uploads\/images\/myco_dna_dish_1.png\" alt=\"\" width=\"120px\" \/><\/a><br \/>\n\u56f3\uff11-a. \u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u30c7\u30a3\u30c3\u30b7\u30e5\u306b\u5165\u308c\u308b \u2192 (1)<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(2)<\/td>\n<td id=\"t1\">Vero\u7d30\u80de\u7528\u57f9\u5730\u3092 (1) \u306edish\u306b2mL\/dish\u5206\u6ce8\u3059\u308b<br \/>\n\u6ce8\uff09\u3053\u306e\u6642\u306b\u30ab\u30d0\u30fc\u30b0\u30e9\u30b9\u3068dish\u5e95\u9762\u3068\u306e\u9593\u306b\u6c17\u6ce1\u304c\u5165\u3089\u306a\u3044\u3088\u3046\u306b\u3059\u308b\n<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(3)<\/td>\n<td id=\"t1\">1-2 x 10<sup>4<\/sup>cells \/ 35mm dish \/ 0.5mL\u3000\u306eVero\u7d30\u80de\u3092\u5747\u4e00\u306b\u306a\u308b\u3088\u3046\u306b (2) \u306edish\u306b\u64ad\u7a2e\u3059\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(4)<\/td>\n<td id=\"t1\">Negative control \u3068\u3057\u3066\u3001\u691c\u4f53\u3092\u52a0\u3048\u306a\u3044Vero\u7d30\u80de\u3092 (3) \u3068\u540c\u69d8\u306b (2) \u306edish\u306b\u64ad\u7a2e\u3057\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u5185\uff0837\u2103\u30015%CO<sub>2<\/sub>\uff09\u306b\u9759\u7f6e\u3059\u308b\u3002<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(5)<\/td>\n<td id=\"t1\"><span style=\"color: #ff0000; font-weight: bold;\">(3) \u306eVero\u7d30\u80de\u3092\u4e00\u6669\u57f9\u990a\u3057\u305f\u5f8c\u3001\u57f9\u5730\u30921mL\u9664\u53bb\u3057\u3001\u305d\u3053\u306b\u691c\u4f53\u30921mL\/dish\u6dfb\u52a0\u3059\u308b<\/span><\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(6)<\/td>\n<td id=\"t1\">\u691c\u4f53\u306e\u6dfb\u52a0\u5f8c<span style=\"color: #ff0000; font-weight: bold;\">5\u30016\u65e5\u9593<\/span>\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u5185\uff0837\u2103\u30015%CO<sub>2<\/sub>\uff09\u306b\u9759\u7f6e\u3059\u308b\u3002<\/td>\n<td id=\"t1\" rowspan=\"5\"><a href=\"\/ja\/wp-content\/uploads\/images\/myco_dna_dish_2.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/myco_dna_dish_2.png\" alt=\"\" width=\"120px\" \/><\/a><br \/>\n\u56f3\uff11-b. \u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u304c\u5165\u3063\u305f\u30c7\u30a3\u30c3\u30b7\u30e5 \u2192 (1)<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(7)<\/td>\n<td id=\"t1\">\u67d3\u8272\u539f\u6db2\u3092PBS(\uff0d)\u3067100\u500d\u306b\u5e0c\u91c8\u3057\u3001\u30a2\u30eb\u30df\u30db\u30a4\u30eb\u3067\u5305\u3093\u306730\u5206\u9593\u30b9\u30bf\u30fc\u30e9\u30fc\u3067\u652a\u62cc\u3059\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(8)<\/td>\n<td id=\"t1\">Negative control \u306eVero\u7d30\u80de\u304a\u3088\u3073\u691c\u4f53\u3092\u6dfb\u52a0\u3057\u305fVero\u7d30\u80de\u306edish\u304b\u3089\u57f9\u5730\u3092\u9664\u53bb\u3059\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(9)<\/td>\n<td id=\"t1\">2mL PBS(\uff0d)\u3067\u6d17\u6d44\u3057\u3001\u4e0a\u6e05\u3092\u9664\u53bb\u3059\u308b<strong>\uff082\uff5e3\u56de\u7e70\u308a\u8fd4\u3059\uff09<\/strong><\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(10)<\/td>\n<td id=\"t1\">PBS(\uff0d)\u5e0c\u91c8\u56fa\u5b9a\u6db2\u30922mL\u52a0\u3048\u300115\u5206\u9593\u56fa\u5b9a\u3057\u305f\u5f8c\u3001 PBS(\uff0d)\u5e0c\u91c8\u56fa\u5b9a\u6db2\u3092\u9664\u53bb\u3059\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(11)<\/td>\n<td id=\"t1\">\u56fa\u5b9a\u6db2\u30922mL\u52a0\u3048\u30015\u5206\u9593\u56fa\u5b9a\u3057\u3001\u56fa\u5b9a\u6db2\u3092\u9664\u53bb\u3059\u308b<\/td>\n<td id=\"t1\" rowspan=\"7\"><a href=\"\/ja\/wp-content\/uploads\/images\/myco_dna_dish_3.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/myco_dna_dish_3.png\" alt=\"\" width=\"120px\" \/><\/a><br \/>\n\u56f32. \u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u30c7\u30a3\u30c3\u30b7\u30e5\u306e\u7e01\u306b\u7acb\u305f\u305b\u3066\u4e7e\u71e5\u3055\u305b\u308b \u2192 (14)<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(12)<\/td>\n<td id=\"t1\">2mL PBS(\uff0d)\u3067\u6d17\u6d44\u3057\u3001\u4e0a\u6e05\u3092\u9664\u53bb\u3059\u308b<strong>\uff083\u56de\u7e70\u308a\u8fd4\u3059\uff09<\/strong><\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(13)<\/td>\n<td id=\"t1\">(7) \u306e100\u500d\u5e0c\u91c8\u3057\u305f\u67d3\u8272\u539f\u6db2\u30922mL\u52a0\u3048\u300110\u5206\u9593\u9759\u7f6e\u3059\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(14)<\/td>\n<td id=\"t1\">\u84b8\u7559\u6c34\u30672\u56de\u6d17\u6d44\u3057\u3001\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u5272\u3089\u306a\u3044\u3088\u3046\u306b\u30d4\u30f3\u30bb\u30c3\u30c8\u3067\u305d\u3063\u3068\u5916\u3057\u3001<span style=\"color: #ff0000; font-weight: bold;\">\u7d30\u80de\u9762\u3092\u4e0a\u306b\u3057\u3066\u4e7e\u71e5\u3055\u305b\u308b<\/span> \uff08\u56f32\uff09<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(15)<\/td>\n<td id=\"t1\">\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u306bMounting solution\u30921\u6ef4\u305a\u3064\u6ef4\u4e0b\u3059\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(16)<\/td>\n<td id=\"t1\">\u67d3\u8272\u5f8c\u3001\u4e7e\u71e5\u3057\u305f\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u7d30\u80de\u304c\u4ed8\u7740\u3057\u305f\u9762\u304c\u4e0b\u306b\u306a\u308b\u3088\u3046\u306b\u3001Mounting solution\u306e\u4e0a\u306b\u88ab\u305b\u308b<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" valign=\"top\">(17)<\/td>\n<td id=\"t1\">\u86cd\u5149\u9855\u5fae\u93e1\uff08Hoechst 33258\u306e\u52b1\u8d77\u5149\uff09\u3067\u86cd\u5149\u89b3\u5bdf\uff08\u63a5\u773c\u30ec\u30f3\u30ba x10\u3001\u5bfe\u7269\u30ec\u30f3\u30ba x40\uff09\u3092\u884c\u3046<br \/>\n\uff08\u63a5\u773c\u30ec\u30f3\u30ba x10\u3001\u5bfe\u7269\u30ec\u30f3\u30ba x100\uff08\u6cb9\u6d78\uff09\u306b\u3088\u308b\u89b3\u5bdf\u304c\u671b\u307e\u3057\u3044\uff09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>3. \u5224\u5b9a<\/strong><br \/>\n\u57f9\u990a\u7d30\u80de\u306b\u5bfe\u3057\u3066\u3001\u7dad\u6301\u57f9\u990a\u4e2d\u306b2\u56de\u4ee5\u4e0a\u306e\u691c\u67fb\u3092\u884c\u3046\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td id=\"t1\">\u30fb\u9670\u6027\uff08\uff0d\uff09\uff1a<\/td>\n<td id=\"t1\">Vero\u7d30\u80de\u306e\u6838\u4ee5\u5916\u306e\u86cd\u5149\u6591\u70b9\u304c\u78ba\u8a8d\u3055\u308c\u306a\u3044\u5834\u5408<\/td>\n<\/tr>\n<tr>\n<td id=\"t1\">\u30fb\u967d\u6027\uff08\uff0b\uff09\uff1a<\/td>\n<td id=\"t1\">\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u3068\u601d\u308f\u308c\u308b\u5fae\u5c0f\u306a\u7403\u72b6\u3001\u6570\u73e0\u72b6\u3001\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u72b6\u7c92\u5b50 \u3042\u308b\u3044\u306f \u3053\u308c\u3089\u306e\u8907\u5408\u4f53\u304c\u6838\u5916\u86cd\u5149\u6591\u70b9\u3068\u3057\u3066\u7d30\u80de\u8cea\u5185\u304a\u3088\u3073\u7d30\u80de\u9593\u9699\u306b\u78ba\u8a8d\u3055\u308c\u305f\u5834\u5408<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><!-- \n\n\n<ul>\n \n\n<li>\u9670\u6027\uff08\uff0d\uff09\uff1aVero\u7d30\u80de\u306e\u6838\u4ee5\u5916\u306e\u86cd\u5149\u6591\u70b9\u304c\u78ba\u8a8d\u3055\u308c\u306a\u3044\u5834\u5408<\/li>\n\n\n \n\n<li>\u967d\u6027\uff08\uff0b\uff09\uff1a\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u3068\u601d\u308f\u308c\u308b\u5fae\u5c0f\u306a\u7403\u72b6\u3001\u6570\u73e0\u72b6\u3001\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u72b6\u7c92\u5b50 \u3042\u308b\u3044\u306f \u3053\u308c\u3089\u306e\u8907\u5408\u4f53\u304c\u6838\u5916\u86cd\u5149\u6591\u70b9\u3068\u3057\u3066\u7d30\u80de\u8cea\u5185\u304a\u3088\u3073\u7d30\u80de\u9593\u9699\u306b\u78ba\u8a8d\u3055\u308c\u305f\u5834\u5408<\/li>\n\n\n<\/ul>\n\n\n --><\/p>\n<table>\n<tbody>\n<tr>\n<td id=\"t1\" style=\"text-align: center;\"><a href=\"\/ja\/wp-content\/uploads\/images\/myco_minus.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/myco_minus.png\" width=\"360px\" \/><\/a><br \/>\n\u5224\u5b9a(\uff0d)<\/td>\n<td id=\"t1\"><\/td>\n<\/tr>\n<tr>\n<td id=\"t1\" style=\"text-align: center;\"><a href=\"\/ja\/wp-content\/uploads\/images\/myco_plus_1.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/myco_plus_1.png\" width=\"360px\" \/><\/a><br \/>\n\u5224\u5b9a(\uff0b)\uff1a\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u72b6<br \/>\n(<em>Mycoplasma fermentans<\/em>)<\/td>\n<td id=\"t1\" style=\"text-align: center;\"><a href=\"\/ja\/wp-content\/uploads\/images\/myco_plus_2.png\"><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/myco_plus_2.png\" width=\"360px\" \/><\/a><br \/>\n\u5224\u5b9a(\uff0b)\uff1a\u7403\u72b6\u7c92\u5b50<br \/>\n(<em>Mycoplasma arginini<\/em>)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u203b \u5f53\u5ba4\u3067\u540c\u5b9a\u3055\u308c\u305f\u57f9\u990a\u7d30\u80de\u306b\u611f\u67d3\u3059\u308b\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306e\u611f\u67d3\u753b\u50cf\u4f8b \uff08\u5bbf\u4e3b\u7d30\u80de\u306f\u5168\u3066Vero\u7d30\u80de\uff09<\/p>\n<ul>\n<li><a href=\"\/ja\/wp-content\/uploads\/pdf\/mycoplasma_orale_dna.pdf\"><em>Mycoplasma orale<\/em><\/a><\/li>\n<li><a href=\"\/ja\/wp-content\/uploads\/pdf\/mycoplasma_hyorhinis_dna.pdf\"><em>Mycoplasma hyorhinis<\/em><\/a><\/li>\n<li><a href=\"\/ja\/wp-content\/uploads\/pdf\/mycoplasma_arginini_dna.pdf\"><em>Mycoplasma arginini<\/em><\/a><\/li>\n<li><a href=\"\/ja\/wp-content\/uploads\/pdf\/mycoplasma_fermentans_dna.pdf\"><em>Mycoplasma fermentans<\/em><\/a><\/li>\n<li><a href=\"\/ja\/wp-content\/uploads\/pdf\/acholeplasma_laidlawii_dna.pdf\"><em>Acholeplasma laidlawii<\/em><\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u203b\u65e5\u672c\u7d44\u7e54\u57f9\u990a\u5b66\u4f1a\u306e\u6a19\u6e96\u30d7\u30ed\u30c8\u30b3\uff0d\u30eb\u306b\u6e96\u3058\u3066\u4e00\u90e8\u6539\u5909 1. \u6e96\u5099\u3059\u308b\u3082\u306e \u691c\u4f53 \u8abf\u6574\u6cd5\u3000\uff1a \u6297\u751f\u7269\u8cea\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u30673\u9031\u9593\u4ee5\u4e0a\u57f9\u990a\u3057\u305f\u7d30\u80de\u3092\u6e96\u5099\u3057\u3001\u7d99\u4ee3\u5f8c\u307e\u305f\u306f\u57f9\u5730\u4ea4\u63db\u5f8c3\u65e5\u4ee5\u4e0a\u7d4c\u904e\u3057\u305f\u57f9\u990a\u4e0a\u6e05\u3092\u63a1\u53d6\u3059\u308b\u203b\u6bce\u65e5\u57f9\u5730\u4ea4\u63db\u3092\u5fc5\u8981\u3068\u3059\u308b\u7d30\u80de\u306e\u5834\u5408\u306f\u7d30\u80de\u304c\u5341\u5206\u306b\u5897\u6b96\u3057\u305f\u30bf\u30a4\u30df\u30f3\u30b0\u3067\u57f9\u5730\u4ea4\u63db\u76f4\u524d\u306e\u57f9\u990a\u4e0a\u6e05\u3092\u7528\u3044\u308b Vero\u7d30\u80de\u3000\uff08\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\u306b\u611f\u67d3\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u6e08\u306e\u3082\u306e\uff09 \u7d30\u80de\u6fc3\u5ea6\u3000\uff1a\u30001-2 x  [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3498,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3406","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3406","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=3406"}],"version-history":[{"count":20,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3406\/revisions"}],"predecessor-version":[{"id":15660,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3406\/revisions\/15660"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3498"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=3406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}