{"id":3383,"date":"2013-04-10T11:25:23","date_gmt":"2013-04-10T02:25:23","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3383"},"modified":"2026-03-11T11:21:17","modified_gmt":"2026-03-11T02:21:17","slug":"fbs_test","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/manual\/fbs_test","title":{"rendered":"\u8840\u6e05(FBS)\u306e\u691c\u5b9a\u6cd5"},"content":{"rendered":"

\u8840\u6e05\u306f\u3001\u88fd\u9020\u5143\u3001Lot\u6bce\u306b\u304b\u306a\u308a\u306e\u5dee\u304c\u3042\u308b\u306e\u3067\u3001\u5fc5\u305a\u691c\u5b9a\u3092\u3057\u3066\u7528\u3044\u308b\u3002\u6570\u7a2e\u985e\u306e\u30c6\u30b9\u30c8\u7528\u30b5\u30f3\u30d7\u30eb\u3092\u696d\u8005\u304b\u3089\u63d0\u4f9b\u3057\u3066\u3082\u3089\u3046\u3002<\/p>\n

\u691c\u5b9a\u306f\u3001\u5897\u6b96\u66f2\u7dda\u3068\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7387\u306e\u7d50\u679c\u3092\u3001\u691c\u5b9a\u6e08\u307f\u306e\u5bfe\u7167(\u5f93\u6765\u54c1\u7b49)\u306e\u8840\u6e05\u3068\u6bd4\u8f03\u3057\u3066\u5224\u5b9a\u3059\u308b\u3002 \u691c\u5b9a\u3059\u308b\u8840\u6e05\u304c\u591a\u6570\u3042\u308b\u5834\u5408\u306f\u3001\u307e\u305aVero\u306e\u5897\u6b96\u66f2\u7dda\u3092\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u6bd4\u8f03\u3057\u30665\u7a2e\u7a0b\u5ea6\u306b\u7d5e\u308b\u3002 \u6b21\u306bL929,NB1RGB\u3092\u7528\u3044\u3066\u540c\u69d8\u306b\u5897\u6b96\u66f2\u7dda\u3092\u3068\u308b\u3002\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7387\u306b\u3088\u308a\u6700\u7d42\u6c7a\u5b9a\u3092\u3059\u308b\u3002<\/p>\n

\u5224\u5b9a\u306b\u969b\u3057\u3066\u306f\u3001\u8840\u6e05\u306e\u8cea\u3084\u5b89\u5b9a\u5316\u3082\u8003\u616e\u3059\u308b\u3002<\/p>\n

\u4f8b\uff1a<\/strong><\/span>\u5897\u6b96\u66f2\u7dda<\/strong><\/span>\u00a0–\u00a0Vero,L929,NB1RGB\u306a\u3069\u3001
\n\u3000\u3000\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u6cd5<\/strong><\/span>\u00a0–\u00a0BALB\/3T3 clone A31,HUC-F\u306a\u3069<\/p>\n

\uff0a\u6ce8\u610f\uff1a<\/strong><\/span>\u4e0b\u306e\u7d30\u80de\u6570\u306f\u53c2\u8003\u306e\u6570\u3067\u3001\u7d30\u80de\u306b\u3088\u3063\u3066\u5909\u3048\u308b\u3053\u3068\u3002<\/p>\n

 <\/p>\n

(a)\u5897\u6b96\u66f2\u7dda<\/span><\/p>\n

    \n
  1. \u57fa\u790e\u57f9\u5730\u306b\u5404\u3005\u306e\u8840\u6e05\u30922\u500d\u6fc3\u5ea6\u542b\u3080\u3088\u3046\u306b\u57f9\u5730\u3092\u8abf\u6574\u3059\u308b\u3002<\/li>\n
  2. \u5404\u3005\u306e\u57f9\u5730\u309235mmdish\u306b1ml\u305a\u3064\u5206\u6ce8\u3057\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u306b\u5165\u308c\u3066\u304a\u304f\u3002
    \n\u3000\u30001\u70b9\u5f53\u305f\u308a2\u679a\u306edish\u3092\u7528\u610f\u3059\u308b\u3002<\/li>\n
  3. \u7d30\u80de\u3092\u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u3059\u308b\u3002
    \n\u3000\u3000\u305f\u3060\u3057\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u306e\u4e2d\u548c\u306b\u306fFBS\u4ee5\u5916\u306e\u8840\u6e05\u3092\u4f7f\u7528\u3059\u308b\u3002<\/li>\n
  4. \u7d30\u80de\u6d6e\u904a\u6db2\u30921000rpm\u30013min\u9060\u5fc3\u3059\u308b\u3002<\/li>\n
  5. \u6c88\u6bbf\u306b\u8840\u6e05\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u3092\u52a0\u3048\u3066\u518d\u3073\u9060\u5fc3\u3092\u3059\u308b\u3002<\/li>\n
  6. \u6c88\u6bbf\u306b\u8840\u6e05\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u3092\u52a0\u3048\u3066\u7d30\u80de\u6570\u3092\u6570\u3048\u308b\u3002<\/li>\n
  7. 3\uff5e5\u00d7104<\/sup><\/span>cells\/ml\u306b\u306a\u308b\u3088\u3046\u306b\u8840\u6e05\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u3067\u5e0c\u91c8\u3059\u308b\u3002\u305f\u3060\u3057\u3001\u7d30\u80de\u306e\u64ad\u7a2e\u6570\u306f\u53c2\u8003\u306e\u5024\u3067\u3042\u308a\u3001\u7d30\u80de\u7a2e\u306b\u3088\u3063\u3066\u5909\u3048\u308b\u3053\u3068\u3002<\/li>\n
  8. (2)\u306e35mm\u30c7\u30a3\u30c3\u30b7\u30e5\u306b1ml\u305a\u3064\u5206\u6ce8\u3059\u308b\u3002<\/li>\n
  9. 1,2,3,4,6\u65e5\u76ee\u306b\u7d30\u80de\u6570\u3092\u6570\u3048\u308b\u3002<\/li>\n<\/ol>\n

     <\/p>\n

    (b) \u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7387<\/span><\/p>\n

    \u7d30\u80de\u306e\u64ad\u7a2e\u6570\u306f\u3001\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u306e\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u80fd\u306b\u3088\u3063\u3066\u52a0\u6e1b\u3059\u308b\u3002
    \n\u7d30\u80de\u7a2e\u306b\u3088\u3063\u3066\u306ffeeder\u7d30\u80de\u304c\u5fc5\u8981\u3068\u306a\u308b\u3002
    \n\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u8840\u6e05\u3067\u300150\uff5e100\u500b\/60mmdish\u306e\u30b3\u30ed\u30cb\u30fc\u6570\u304c\u6570\u3048\u5b89\u304f\u3001\u3070\u3089\u3064\u304d\u304c\u5c11\u306a\u3044\u306e\u3067\u4fe1\u983c\u3067\u304d\u308b\u3002<\/p>\n

      \n
    1. \u57fa\u790e\u57f9\u5730\u306b\u5404\u3005\u306e\u8840\u6e05\u30922\u500d\u6fc3\u5ea6<\/strong><\/span>\u542b\u3080\u3088\u3046\u306b\u57f9\u5730\u3092\u8abf\u6574\u3059\u308b\u3002<\/li>\n
    2. \u5404\u3005\u306e\u57f9\u5730\u309260mmdish\u306b2.5ml\u305a\u3064\u5206\u6ce8\u3057\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u5165\u308c\u3066\u304a\u304f\u3002
      \n 1\u30b5\u30f3\u30d7\u30eb\u5f53\u305f\u308a5\u679a\u306edish\u3092\u7528\u610f\u3059\u308b\u3002<\/li>\n<\/ol>\n

       <\/p>\n\n\n\n
      \uff1cfeeder\u7d30\u80de\u304c\u5fc5\u8981\u306a\u5834\u5408\uff1e<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
      (3)<\/td>\n\u7d30\u80de\u306bX\u7dda\u3092\u7167\u5c04\u3059\u308b\u3002(5000R)<\/td>\n<\/tr>\n
      (4)<\/td>\n\u7167\u5c04\u5f8c\u3001\u7d30\u80de\u3092\u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u3059\u308b\u3002\u4f46\u3057\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u4e2d\u548c\u306b\u306fFBS\u4ee5\u5916\u306e\u8840\u6e05\u3092\u4f7f\u7528\u3002<\/td>\n<\/tr>\n
      (5)<\/td>\n\u7d30\u80de\u6d6e\u904a\u6db2\u30921000 rpm\u30013min \u9060\u5fc3\u3002<\/td>\n<\/tr>\n
      (6)<\/td>\n\u6c88\u6bbf(\u7d30\u80de)\u306b\u8840\u6e05\u4e0d\u542b\u57f9\u5730\u3092\u52a0\u3048\u3066\u518d\u3073\u9060\u5fc3\u3002<\/td>\n<\/tr>\n
      (7)<\/td>\n\u6c88\u6bbf\u306b\u8840\u6e05\u4e0d\u542b\u57f9\u5730\u3092\u52a0\u3048\u3066\u3001\u7d30\u80de\u6570\u3092\u6570\u3048\u308b\u3002<\/td>\n<\/tr>\n
      (8)<\/td>\n2\uff5e3×104<\/sup><\/span>cells\/1.5ml\u306b\u3001\u8840\u6e05\u4e0d\u542b\u57f9\u5730\u3067\u5e0c\u91c8\u3002<\/td>\n<\/tr>\n
      (9)<\/td>\n(2)\u306e60mmdish\u306b\u30011.5ml\u305a\u3064\u64ad\u7a2e\u3057\u518d\u3073\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u5165\u308c\u308b\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n\uff1cfeeder\u7d30\u80de\u304c\u5fc5\u8981\u3067\u306a\u3044\u5834\u5408\uff1e<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
      (10)<\/td>\n\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7528
      \n(2)\u306e60mmdish\u306b\u8840\u6e05\u3092\u542b\u307e\u306a\u3044\u57f9\u5730\u30921.5ml\u305a\u3064\u5206\u6ce8\u3057\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u3078\u3002<\/td>\n<\/tr>\n
      (11)<\/td>\n\u7d30\u80de\u3092\u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u3059\u308b\u3002\u4f46\u3057\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u4e2d\u548c\u306b\u306fFBS\u4ee5\u5916\u306e\u8840\u6e05\u4f7f\u7528\u3002<\/td>\n<\/tr>\n
      (12)<\/td>\n\u7d30\u80de\u6d6e\u904a\u6db2\u30921000rpm,3min\u9060\u5fc3\u3002<\/td>\n<\/tr>\n
      (13)<\/td>\n\u6c88\u6bbf\u306b\u8840\u6e05\u4e0d\u542b\u57f9\u5730\u3092\u52a0\u3048\u3066\u518d\u3073\u9060\u5fc3\u3002<\/td>\n<\/tr>\n
      (14)<\/td>\n\u6c88\u6bbf\u306b\u8840\u6e05\u4e0d\u542b\u57f9\u5730\u3092\u6dfb\u52a0\u3002<\/td>\n<\/tr>\n
      (15)<\/td>\n150\uff5e300cells\/ml\u306b\u8840\u6e05\u4e0d\u542b\u57f9\u5730\u3067\u5e0c\u91c8\u3002<\/td>\n<\/tr>\n
      (16)<\/td>\n(9)\u53c8\u306f(10)\u306e60mmdish\u306b\u30011ml\u305a\u3064\u64ad\u7a2e\u3002<\/td>\n<\/tr>\n
      (17)<\/td>\n10\uff5e14\u65e5\u9593\u57f9\u990a\u5f8c\u3001\u56fa\u5b9a\u67d3\u8272\u3002<\/td>\n<\/tr>\n
      (18)<\/td>\n50\u500b\u4ee5\u4e0a\u306e\u7d30\u80de\u6570\u304c\u3042\u308b\u30b3\u30ed\u30cb\u30fc\u30921\u500b\u3068\u6570\u3048\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7387\u3092\u6c42\u3081\u308b\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \u203b\u305d\u306e\u4ed6\u306e\u8840\u6e05\u306b\u3064\u3044\u3066\u3082\u3001\u305d\u306e\u8840\u6e05\u3092\u7528\u3044\u308b\u7d30\u80de\u3092\u7528\u3044\u3066\u540c\u69d8\u306b\u884c\u3046\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

      \u8840\u6e05\u306f\u3001\u88fd\u9020\u5143\u3001Lot\u6bce\u306b\u304b\u306a\u308a\u306e\u5dee\u304c\u3042\u308b\u306e\u3067\u3001\u5fc5\u305a\u691c\u5b9a\u3092\u3057\u3066\u7528\u3044\u308b\u3002\u6570\u7a2e\u985e\u306e\u30c6\u30b9\u30c8\u7528\u30b5\u30f3\u30d7\u30eb\u3092\u696d\u8005\u304b\u3089\u63d0\u4f9b\u3057\u3066\u3082\u3089\u3046\u3002 \u691c\u5b9a\u306f\u3001\u5897\u6b96\u66f2\u7dda\u3068\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7387\u306e\u7d50\u679c\u3092\u3001\u691c\u5b9a\u6e08\u307f\u306e\u5bfe\u7167(\u5f93\u6765\u54c1\u7b49)\u306e\u8840\u6e05\u3068\u6bd4\u8f03\u3057\u3066\u5224\u5b9a\u3059\u308b\u3002 \u691c\u5b9a\u3059\u308b\u8840\u6e05\u304c\u591a\u6570\u3042\u308b\u5834\u5408\u306f\u3001\u307e\u305aVero\u306e\u5897\u6b96\u66f2\u7dda\u3092\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3068\u6bd4\u8f03\u3057\u30665\u7a2e\u7a0b\u5ea6\u306b\u7d5e\u308b\u3002 \u6b21\u306bL929,NB1RGB\u3092\u7528\u3044\u3066\u540c\u69d8\u306b\u5897\u6b96\u66f2\u7dda\u3092\u3068\u308b\u3002\u30b3\u30ed\u30cb\u30fc\u5f62\u6210\u7387\u306b\u3088\u308a\u6700\u7d42\u6c7a\u5b9a\u3092\u3059 […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3341,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3383","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3383","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=3383"}],"version-history":[{"count":17,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3383\/revisions"}],"predecessor-version":[{"id":15648,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3383\/revisions\/15648"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3341"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=3383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}