{"id":3379,"date":"2013-04-10T10:49:00","date_gmt":"2013-04-10T01:49:00","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3379"},"modified":"2021-12-24T16:14:32","modified_gmt":"2021-12-24T07:14:32","slug":"toketsu","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/manual\/toketsu","title":{"rendered":"\u7d30\u80de\u306e\u51cd\u7d50\u65b9\u6cd5 (\u7de9\u6162\u51b7\u5374\u6cd5\uff1aSlow freezing)"},"content":{"rendered":"<p>\u7d30\u80de\u306e\u56de\u53ce<\/p>\n<blockquote style=\"width: 600px;\"><p><span style=\"color: #ff4433;\"><strong>\u5bfe\u6570\u5897\u6b96\u671f<\/strong><\/span>\u306e\u7d30\u80de\u3092\u56de\u53ce\u3057\u3001<span style=\"color: #ff4433;\"><strong>\u9060\u5fc3\u30001000 rpm\u30013 min \u5ba4\u6e29<\/strong><\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_below.png\" width=\"16\" height=\"16\" \/><\/p>\n<p><span style=\"color: #ff4433;\"><strong>Culture Medium\uff0b10%DMSO<\/strong><\/span>\u306b<span style=\"color: #ff4433;\"><strong>1.0\u00d710<span style=\"font-size: small;\"><sup>6<\/sup><\/span>\u500b\u4ee5\u4e0a<\/strong><\/span><br \/>\n(\u91cf\u304c\u591a\u3051\u308c\u30702.0\u00d710<span style=\"font-size: small;\"><sup>6<\/sup><\/span>\u4ee5\u4e0a)\/mL\u3068\u306a\u308b\u3088\u3046\u306b\u8abf\u88fd\u3059\u308b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_below.png\" width=\"16\" height=\"16\" \/><\/p>\n<p>\u30d7\u30ed\u30b0\u30e9\u30e0\u30d5\u30ea\u30fc\u30b6\u30fc\u304c\u3042\u308c\u3070\u3001<span style=\"color: #ff4433;\"><strong>-80\u2103\u307e\u3067\u3086\u3063\u304f\u308a\u51b7\u5374<\/strong><\/span>\u3059\u308b\u3002<br \/>\n(10\uff5e15\u2103\u306e\u8a66\u6599\u30922\u2103\/\u5206\u306e\u901f\u5ea6\u30674\u2103\u307e\u3067\u51b7\u5374\u3057\u306610\u5206\u4fdd\u6301\u3057\u3001<br \/>\n\u7d9a\u3044\u30661\u2103\/\u5206\u3067-30\u2103\u307e\u3067\u76f4\u7dda\u7684\u306b\u51b7\u5374\u305710\u5206\u4fdd\u6301\u3057\u3001<br \/>\n\u3055\u3089\u306b5\u2103\/\u5206\u3067-80\u2103\u307e\u3067\u51b7\u5374\u3057\u307e\u3059\u3002)<br \/>\n\u307e\u305f\u306f\u5e02\u8ca9\u306e\u51cd\u7d50\u5c02\u7528\u5bb9\u5668\u306b\u5165\u308c\u3001-80\u2103\u306e\u30d5\u30ea\u30fc\u30b6\u30fc\u3067\u4e00\u6669\u653e\u7f6e\u3057\u307e\u3059\u3002<br \/>\n\u3053\u306e\u72b6\u614b\u3067\u306e\u4fdd\u5b58\u306f\u30011\u9031\u9593\u4ee5\u5185\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_below.png\" width=\"16\" height=\"16\" \/><\/p>\n<p>-80\u2103\u3067\u51cd\u3063\u305f\u7d30\u80de\u3092\u51b7\u51cd\u5eab\u304b\u3089\u53d6\u308a\u51fa\u3057\u3001<span style=\"color: #ff4433;\"><strong>\u3059\u3070\u3084\u304f\u6db2\u4f53\u7a92\u7d20\u306b\u5165\u308c\u3066\u3001\u4fdd\u5b58<\/strong><\/span>\u3059\u308b\u3002<br \/>\n\u6db2\u4f53\u7a92\u7d20\u4e2d\u306a\u3089\u3070\u3001\u534a\u6c38\u4e45\u7684\u306b\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>\u7d30\u80de\u306e\u56de\u53ce \u5bfe\u6570\u5897\u6b96\u671f\u306e\u7d30\u80de\u3092\u56de\u53ce\u3057\u3001\u9060\u5fc3\u30001000 rpm\u30013 min \u5ba4\u6e29 Culture Medium\uff0b10%DMSO\u306b1.0\u00d7106\u500b\u4ee5\u4e0a (\u91cf\u304c\u591a\u3051\u308c\u30702.0\u00d7106\u4ee5\u4e0a)\/mL\u3068\u306a\u308b\u3088\u3046\u306b\u8abf\u88fd\u3059\u308b\u3002 \u30d7\u30ed\u30b0\u30e9\u30e0\u30d5\u30ea\u30fc\u30b6\u30fc\u304c\u3042\u308c\u3070\u3001-80\u2103\u307e\u3067\u3086\u3063\u304f\u308a\u51b7\u5374\u3059\u308b\u3002 (10\uff5e15\u2103\u306e\u8a66\u6599\u30922\u2103\/\u5206\u306e\u901f\u5ea6\u30674\u2103\u307e\u3067\u51b7\u5374\u3057\u306610\u5206\u4fdd\u6301\u3057\u3001 \u7d9a\u3044\u30661\u2103\/\u5206\u3067-30\u2103\u307e\u3067\u76f4\u7dda\u7684\u306b\u51b7\u5374\u305710\u5206\u4fdd\u6301\u3057\u3001 [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3341,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3379","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3379","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=3379"}],"version-history":[{"count":5,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3379\/revisions"}],"predecessor-version":[{"id":13139,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3379\/revisions\/13139"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3341"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=3379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}