{"id":3362,"date":"2013-04-10T10:15:10","date_gmt":"2013-04-10T01:15:10","guid":{"rendered":"http:\/\/cell.brc.riken.jp:7321\/?page_id=3362"},"modified":"2022-06-03T09:35:36","modified_gmt":"2022-06-03T00:35:36","slug":"passage","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/manual\/passage","title":{"rendered":"\u7d30\u80de\u306e\u7d99\u4ee3\u65b9\u6cd5"},"content":{"rendered":"<p><strong><span style=\"color: #3344ff;\"><strong>\u3010\u4ed8\u7740\u7d30\u80de\u3011<\/strong><\/span><\/strong><\/p>\n<p><strong>1. \u6e96\u5099\u3059\u308b\u3082\u306e<\/strong><\/p>\n<ul>\n<li>\u5bfe\u8c61\uff1a\u7d30\u80de\n<li>\u57f9\u5730\n<li>PBS\uff08-\uff09\n<li>\u30c8\u30ea\u30d7\u30b7\u30f3\u7b49\n<li>\u57f9\u990a\u5bb9\u5668\u30fb\u57f9\u990a\u5668\u5177\u30fb\u6a5f\u5668\u7b49\n<\/ul>\n<p><strong>2. \u7d99\u4ee3\u64cd\u4f5c<\/strong><\/p>\n<ol class=\"list_parentheses\">\n<li> \u57f9\u5730\u30fbPBS\uff08-\uff09\u3092\u5ba4\u6e29\u306b\u623b\u3059\u3002\n<li> \u9855\u5fae\u93e1\u89b3\u5bdf\u306b\u3088\u308a\u3001\u7d30\u80de\u304c80\uff5e90\uff05\u7a0b\u5ea6\u306e\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u3002\n<li> \u7d30\u80de\u8868\u9762\u3092\u50b7\u3064\u3051\u306a\u3044\u3088\u3046\u306b\u5bb9\u5668\u3092\u50be\u3051\u306a\u304c\u3089\u53e4\u3044\u57f9\u5730\u3092\u9664\u304d\u3001PBS\uff08-\uff09\u3092\u57f9\u990a\u5bb9\u5668\u306e\u58c1\u306b\u6cbf\u3063\u3066\u9759\u304b\u306b\u5165\u308c\u3001\u5bb9\u5668\u3092\u3086\u3063\u304f\u308a2\uff5e3\u56de\u50be\u3051\u3066\u7d30\u80de\u8868\u9762\u3092\u6d17\u3063\u305f\u3042\u3068PBS\uff08-\uff09\u3092\u9664\u304f\u3002\uff08\uff582\uff09<br \/>\u3000\u3000<span style=\"color: red\">\u6ce8\uff09\u57f9\u5730\u3084PBS\uff08-\uff09\u3092\u9664\u3044\u305f\u969b\u306b\u7d30\u80de\u8868\u9762\u304c\u4e7e\u3044\u3066\u3057\u307e\u3046\u3068\u3001\u7d30\u80de\u304c\u6b7b\u3093\u3067\u3057\u307e\u3046\u306e\u3067\u6ce8\u610f\u3059\u308b\u3002<\/span>\n<li>\u30c8\u30ea\u30d7\u30b7\u30f3\u7b49\u3092\u9069\u91cf\u6dfb\u52a0\u3057\u3001\u5bb9\u5668\u3092\u50be\u3051\u308b\u306a\u3069\u3057\u3066\u3001\u7d30\u80de\u5168\u4f53\u306b\u884c\u304d\u308f\u305f\u3089\u305b\u305f\u5f8c\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b\u3002<br \/>\u3000\u3000\uff0860mmdish\u306e\u5834\u5408\u306f0.5ml\u7a0b\u5ea6\uff09\n<li>\u9855\u5fae\u93e1\u3067\u7d30\u80de\u304c\u4e38\u304f\u306a\u308a\u5265\u304c\u308c\u59cb\u3081\u305f\u306e\u3092\u78ba\u8a8d\u3059\u308b\u3002<br \/>\u3000\u3000<span style=\"color: red\">\u6ce8\uff09\u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u306e\u6642\u9593\u304c\u9577\u3059\u304e\u308b\u3068\u7d30\u80de\u306e\u751f\u5b58\u306b\u5f71\u97ff\u3059\u308b\u3002<\/span>\n<li> \u8840\u6e05\u5165\u308a\u57f9\u5730\u7b49\u3092\u52a0\u3048\u30c8\u30ea\u30d7\u30b7\u30f3\u306e\u6d3b\u6027\u3092\u6b62\u3081\u3001\u7a4f\u3084\u304b\u306b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3044\u3001\u7d30\u80de\u3092\u57f9\u990a\u5bb9\u5668\u304b\u3089\u5265\u304c\u3059\u3068\u540c\u6642\u306b\u7d30\u80de\u3092\u5206\u6563\u3055\u305b\u308b\u3002<span style=\"color: red\">Trypsin-EDTA\uff08\u203b\uff09<\/span>\u4f7f\u7528\u6642\u306f\u9060\u5fc3\u3057\u3066Trypsin-EDTA\u3092\u53d6\u308a\u9664\u304f\u3002<br \/>\u3000\u3000<span style=\"color: red\">\uff08\u203b\uff09\u7d30\u80de\u3092\u5168\u3066\u56de\u53ce\u3057\u3066\u9060\u5fc3\u5f8c\uff081000rpm\uff583\uff5e5min \u5ba4\u6e29\uff09\u3001\u4e0a\u6e05\u3092\u6368\u3066\u3001\u65b0\u3057\u3044\u57f9\u5730\u3067\u7d30\u80de\u3092\u518d\u61f8\u6fc1\u3059\u308b\u3002<\/span>\n<li>\u65b0\u3057\u3044\u57f9\u990a\u5bb9\u5668\u306b\u57f9\u5730\u3092\u5165\u308c\u3001\u7d30\u80de\u61f8\u6fc1\u6db2\u3092\u64ad\u7a2e\u3059\u308b\u3002\uff08\u7d99\u4ee3\u5bc6\u5ea6\u306f\u30c7\u30fc\u30bf\u30b7\u30fc\u30c8\u53c2\u7167\uff09<br \/>\u3000\u3000\u7d99\u4ee3\u5bc6\u5ea6\u306f\u7d30\u80de\u306e\u72b6\u614b\u306b\u3088\u3063\u3066\u8abf\u6574\u3059\u308b\u3002\n<li> \u57f9\u990a\u5bb9\u5668\u3092\u8efd\u304f\u63fa\u3059\u308a\u3001\u7d30\u80de\u3092\u5747\u4e00\u306b\u3059\u308b\u3002\n<li> \u9855\u5fae\u93e1\u3067\u7d30\u80de\u3092\u78ba\u8a8d\u3059\u308b\u3002\n<li> \u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u5165\u308c\u308b\u3002\uff08\u6e29\u5ea6\u306f\u30c7\u30fc\u30bf\u30b7\u30fc\u30c8\u53c2\u7167\uff09\n<\/ol>\n<p><a href=\"http:\/\/ch.nicovideo.jp\/rbrc\">\u30cb\u30b3\u30cb\u30b3\u52d5\u753b RikenBRC\u30c1\u30e3\u30f3\u30cd\u30eb<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/niconico_s.png\" \/><\/a><\/p>\n<table>\n<tbody>\n<tr>\n<td align=\"center\"><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378952884\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"aligncenter\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/mov\/pass_01.png\" \/><\/a><\/td>\n<td align=\"center\"><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378953491\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"aligncenter\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/mov\/med_01.png\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378952884\" target=\"_blank\" rel=\"noopener\">6.\u7d99\u4ee3\u64cd\u4f5c\uff08\u4ed8\u7740\u7d30\u80de\uff09[1]-[4]<\/a><\/td>\n<td><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378953491\" target=\"_blank\" rel=\"noopener\">7.\u57f9\u5730\u4ea4\u63db\uff08\u4ed8\u7740\u7d30\u80de\uff09<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><span style=\"color: #3344ff;\"><strong>\u3010\u6d6e\u904a\u7d30\u80de\u3011<\/strong><\/span><\/strong><\/p>\n<p><strong>1. \u6e96\u5099\u3059\u308b\u3082\u306e<\/strong><\/p>\n<ul>\n<li>\u5bfe\u8c61\uff1a\u7d30\u80de\n<li>\u57f9\u5730\n<li>\u57f9\u990a\u5bb9\u5668\u30fb\u57f9\u990a\u5668\u5177\u30fb\u6a5f\u5668\u7b49\n<\/ul>\n<p><strong>2. \u7d99\u4ee3\u64cd\u4f5c<\/strong><\/p>\n<ol class=\"list_parentheses\">\n<li> \u57f9\u5730\u3092\u5ba4\u6e29\u306b\u623b\u3059\u3002\n<li> \u9855\u5fae\u93e1\u89b3\u5bdf\u306b\u3088\u308a\u3001\u7d30\u80de\u304c80\uff5e90\uff05\u7a0b\u5ea6\u306e\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u3002<br \/>\n\u3000 \uff1c\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u306e\u898b\u6975\u3081\u65b9\uff1e<br \/>\n\u3000\u30fb\u76ee\u5b89\u3068\u3057\u3066\u306f\u57f9\u5730\u304c\u3084\u3084\u9ec4\u8272\u304f\u306a\u308b\u3002<br \/>\n\u3000\u30fb\u4e0b\u306e\u5199\u771f\u53c2\u7167\u3002<\/p>\n<li> \u57f9\u990a\u6db2\u3092\u7a4f\u3084\u304b\u306b\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3057\u3001\u7d30\u80de\u3092\u5206\u6563\u3055\u305b\u308b\u3002\n<li> \u65b0\u3057\u3044\u57f9\u990a\u5bb9\u5668\u306b\u57f9\u5730\u3092\u5165\u308c\u3001\u7d30\u80de\u61f8\u6fc1\u6db2\u3092\u64ad\u7a2e\u3059\u308b\u3002\uff08\u7d99\u4ee3\u5bc6\u5ea6\u306f\u30c7\u30fc\u30bf\u30b7\u30fc\u30c8\u53c2\u7167\uff09\n<li> \u57f9\u990a\u5bb9\u5668\u3092\u8efd\u304f\u63fa\u3059\u308a\u3001\u7d30\u80de\u3092\u5747\u4e00\u306b\u3059\u308b\u3002\n<li> \u9855\u5fae\u93e1\u3067\u7d30\u80de\u3092\u78ba\u8a8d\u3059\u308b\u3002\n<li> \u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u5165\u308c\u308b\u3002\uff08\u6e29\u5ea6\u306f\u30c7\u30fc\u30bf\u30b7\u30fc\u30c8\u53c2\u7167\uff09\n<\/ol>\n<p><img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/rcb0041_confluent.jpg\" alt=\"RCB0041\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\" width=\"384px\"><\/a><\/p>\n<p><a href=\"http:\/\/ch.nicovideo.jp\/rbrc\">\u30cb\u30b3\u30cb\u30b3\u52d5\u753b RikenBRC\u30c1\u30e3\u30f3\u30cd\u30eb<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/niconico_s.png\" \/><\/a><\/p>\n<table>\n<tbody>\n<tr>\n<td align=\"center\"><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378953608\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"aligncenter\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/mov\/pass_02.png\" \/><\/a><\/td>\n<td align=\"center\"><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378958349\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"aligncenter\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/mov\/med_02.png\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378953608\" target=\"_blank\" rel=\"noopener\">8.\u7d99\u4ee3\u64cd\u4f5c\uff08\u6d6e\u904a\u7d30\u80de\uff09[1]-[3]<\/a><\/td>\n<td><a href=\"http:\/\/www.nicovideo.jp\/watch\/1378958349\" target=\"_blank\" rel=\"noopener\">9.\u57f9\u5730\u4ea4\u63db\uff08\u6d6e\u904a\u7d30\u80de\uff09[1]-[2]<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u4ed8\u7740\u7d30\u80de\u3011 1. \u6e96\u5099\u3059\u308b\u3082\u306e \u5bfe\u8c61\uff1a\u7d30\u80de \u57f9\u5730 PBS\uff08-\uff09 \u30c8\u30ea\u30d7\u30b7\u30f3\u7b49 \u57f9\u990a\u5bb9\u5668\u30fb\u57f9\u990a\u5668\u5177\u30fb\u6a5f\u5668\u7b49 2. \u7d99\u4ee3\u64cd\u4f5c \u57f9\u5730\u30fbPBS\uff08-\uff09\u3092\u5ba4\u6e29\u306b\u623b\u3059\u3002 \u9855\u5fae\u93e1\u89b3\u5bdf\u306b\u3088\u308a\u3001\u7d30\u80de\u304c80\uff5e90\uff05\u7a0b\u5ea6\u306e\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u3002 \u7d30\u80de\u8868\u9762\u3092\u50b7\u3064\u3051\u306a\u3044\u3088\u3046\u306b\u5bb9\u5668\u3092\u50be\u3051\u306a\u304c\u3089\u53e4\u3044\u57f9\u5730\u3092\u9664\u304d\u3001PBS\uff08-\uff09\u3092\u57f9\u990a\u5bb9\u5668\u306e\u58c1\u306b\u6cbf\u3063\u3066\u9759\u304b\u306b\u5165\u308c\u3001\u5bb9\u5668\u3092\u3086\u3063\u304f\u308a2\uff5e3\u56de\u50be\u3051\u3066\u7d30\u80de\u8868\u9762\u3092\u6d17\u3063\u305f\u3042\u3068PBS\uff08- [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":3341,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3362","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3362","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=3362"}],"version-history":[{"count":20,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3362\/revisions"}],"predecessor-version":[{"id":13247,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3362\/revisions\/13247"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3341"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=3362"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}