{"id":13984,"date":"2023-12-18T11:51:04","date_gmt":"2023-12-18T02:51:04","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/ja\/?page_id=13984"},"modified":"2024-11-06T10:40:41","modified_gmt":"2024-11-06T01:40:41","slug":"notes_on_culture_of_suspension_cells","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/manual\/notes_on_culture_of_suspension_cells","title":{"rendered":"\u6d6e\u904a\u7d30\u80de\u306e\u57f9\u990a\u306b\u95a2\u3059\u308b\u4e00\u822c\u7684\u306a\u6ce8\u610f"},"content":{"rendered":"<p style=\"text-align: right;\"><a href=\"\/ja\/wp-content\/uploads\/pdf\/notes_on_culture_of_suspension_cells.pdf\">PDF\u7248\u306f\u3053\u3061\u3089<img decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" valign=\"middle\" border=\"0\"><\/a><\/p>\n<p><font color=\"red\">\u203b \u30d2\u30c8\u8840\u7403\u7cfb\u7d30\u80de\u306a\u3069\u3001\u6d6e\u904a\u7d30\u80de\u306e\u4e2d\u306b\u306f \u7279\u306b\u57f9\u990a\u306e\u96e3\u3057\u3044\u682a\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\u57f9\u990a\u306e\u96e3\u3057\u3044\u7d30\u80de\u682a\u306b\u3064\u3044\u3066\u306f\u3001\u682a\u6bce\u306b\u5225\u9014\u306e\u300c\u57f9\u990a\u958b\u59cb\u306b\u3042\u305f\u3063\u3066\u306e\u6ce8\u610f\u300d\u3092\u3054\u6848\u5185\u3057\u3066\u304a\u308a\u307e\u3059\u306e\u3067\u3001\u57f9\u990a\u524d\u306b\u5fc5\u305a\u304a\u8aad\u307f\u304f\u3060\u3055\u3044\u3002<\/font><\/p>\n<hr \/>\n<p><b>\u6e96\u5099<\/b><\/p>\n<ul>\n<li>\u4e88\u308137\u2103\u306e\u30a6\u30a9\u30fc\u30bf\u30fc\u30d0\u30b9\uff08\u307e\u305f\u306f \u5e02\u8ca9\u306e\u30a6\u30a9\u30fc\u30bf\u30fc\u30d5\u30ea\u30fc\u7d30\u80de\u878d\u89e3\u88c5\u7f6e\uff09\u306e\u96fb\u6e90\u3092\u5165\u308c\u3066\u6e96\u5099\u3057\u3066\u304a\u304d\u307e\u3059\u3002<br \/>\n\u203b\u6642\u3005\u3001\u51cd\u7d50\u30c1\u30e5\u30fc\u30d6\u3092\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u9759\u7f6e\u3057\u3066\u878d\u89e3\u3092\u884c\u3044\u3001\u7d30\u80de\u3092\u5168\u6ec5\u3055\u305b\u3066\u3057\u307e\u3046\u5931\u6557\u4f8b\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u878d\u89e3\u64cd\u4f5c\u306f\u5fc5\u305a37\u2103\u306e\u30a6\u30a9\u30fc\u30bf\u30fc\u30d0\u30b9\u304b\u5c02\u7528\u306e\u7d30\u80de\u878d\u89e3\u88c5\u7f6e\u3092\u304a\u4f7f\u3044\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u4f7f\u7528\u3059\u308b\u57f9\u5730\u306f\u4e8b\u524d\u306b\u6e96\u5099\u3057\u3001\u5ba4\u6e29\u306b\u623b\u3057\u3066\u304a\u304d\u307e\u3059\u3002<br \/>\n\u904e\u5ea6\u306a\u52a0\u6e29\u306f\u57f9\u5730\u6210\u5206\u3092\u5909\u5316\u30fb\u5931\u6d3b\u3055\u305b\u3001\u7d30\u80de\u306e\u5897\u6b96\u4e0d\u826f\u306e\u4e00\u56e0\u3068\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<li>\u57f9\u5730\u306b\u6297\u751f\u7269\u8cea\u306eAmphotericin B\u3092\u6dfb\u52a0\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u3001\u5897\u6b96\u304c\u4e0a\u624b\u304f\u3044\u304b\u306a\u304f\u306a\u308b\u4e8b\u4f8b\u304c\u898b\u3089\u308c\u307e\u3059\u3002<br \/>\n\u8907\u6570\u306e\u6297\u751f\u7269\u8cea\u304c\u4e88\u3081\u6df7\u5408\u3055\u308c\u305f\u5e02\u8ca9\u54c1\u306b\u306fAmphotericin B\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u4e8b\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u6297\u751f\u7269\u8cea\u3092\u5229\u7528\u3055\u308c\u308b\u5834\u5408\u306f\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u9060\u5fc3\u6a5f\u306f\u51b7\u5374\u305b\u305a\u306b\u5ba4\u6e29\u3067\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<hr 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href=\"\/ja\/manual\/thawing_of_frozen_ampoules\">\u9001\u4ed8\u3055\u308c\u305f\u7d30\u80de\u306e\u878d\u89e3\u30fb\u57f9\u990a\u65b9\u6cd5\u3092\u53c2\u7167<\/a><\/p>\n<li>\u878d\u89e3\u64cd\u4f5c\u306f\u5fc5\u305a<b>1\u672c\u305a\u3064<\/b>\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li><b>\u878d\u89e3\u6642\u9593<\/b>\u304c\u7d30\u80de\u306e\u751f\u5b58\u7387\u306b\u5927\u304d\u304f\u5f71\u97ff\u3057\u307e\u3059\u3002<\/li>\n<p>\u203b\u7d30\u80de\u306e\u51cd\u7d50\u4fdd\u5b58\u306f\u300c\u7de9\u6162\u51cd\u7d50-\u6025\u901f\u878d\u89e3\u300d\u304c\u57fa\u672c\u3067\u3059\u3002<br \/>\n\u878d\u89e3\u6642\u9593\u3068\u306f\u3001\u7d30\u80de\u3092\u50b7\u5bb3\u3059\u308b\u6c37\u7d50\u6676\u6838\u304c\u751f\u6210\u30fb\u6210\u9577\u3059\u308b-80\u2103\u4ee5\u4e0a\u306e\u6e29\u5ea6\u5e2f\u3092\u5982\u4f55\u306b\u901f\u3084\u304b\u306b\u901a\u904e\u3057\u3066\u878d\u89e3\u3067\u304d\u308b\u304b\u3092\u610f\u5473\u3057\u3066\u3044\u307e\u3059\u3002<br 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href=\"https:\/\/scholar.google.co.jp\/scholar?hl=ja&#038;as_sdt=0%2C5&#038;q=DMSO+toxicity+blood+cell+line+culture&#038;btnG=\">https:\/\/scholar.google.co.jp\/scholar?hl=ja&#038;as_sdt=0%2C5&#038;q=DMSO+toxicity+blood+cell+line+culture&#038;btnG=<\/a><br \/>\n\u878d\u89e3\u5f8c\u3001DMSO\u3092\u542b\u3080\u51cd\u7d50\u4fdd\u5b58\u6db2\u306e\u307e\u307e\u52a0\u6e29\u3055\u308c\u308b \u3042\u308b\u3044\u306f DMSO\u306b\u66dd\u3055\u308c\u3066\u3044\u308b\u6642\u9593\u304c\u9577\u304f\u306a\u308b\u3068\u3001\u7d30\u80de\u306e\u751f\u5b58\u7387\u306f\u3069\u3093\u3069\u3093\u4f4e\u4e0b\u3057\u3001\u5834\u5408\u306b\u3088\u3063\u3066\u306f\u5168\u6ec5\u3057\u307e\u3059\u3002<\/p>\n<p>\u3000\u203bDMSO\u306b\u306f\u7d30\u80de\u306e\u5206\u5316\u8a98\u5c0e\u3092\u4fc3\u9032\u3059\u308b\u4f5c\u7528\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n<a href=\"https:\/\/scholar.google.co.jp\/scholar?hl=ja&#038;as_sdt=0%2C5&#038;q=DMSO+differentiation+blood+cell+line+culture&#038;btnG=\">https:\/\/scholar.google.co.jp\/scholar?hl=ja&#038;as_sdt=0%2C5&#038;q=DMSO+differentiation+blood+cell+line+culture&#038;btnG=<\/a><br \/>\n\u7d30\u80de\u306b\u3088\u3063\u3066\u306f\u878d\u89e3\u6642\u306bDMSO\u304c\u6b8b\u5b58\u3057\u3066\u3044\u308b\u3068\u5206\u5316\u304c\u8a98\u5c0e\u3055\u308c\u3001\u305d\u306e\u682a\u306e\u7279\u6027\u304c\u5909\u5316\u3057\u3066\u3057\u307e\u3046\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u3053\u3053\u3067\u6700\u3082\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u306f\u3001\u878d\u89e3\u76f4\u5f8c\u306e\u307e\u3060\u7d30\u80de\u306e\u4ee3\u8b1d\u304c\u6291\u5236\u3055\u308c\u3066\u3044\u308b\u9593\u306b<b>\u7d20\u65e9\u304f<\/b>\u3001<b>\u3057\u3063\u304b\u308a<\/b>\u3068\u306a\u304a\u304b\u3064<b>\u3084\u3055\u3057\u304fDMSO\u6fc3\u5ea6\u3092\u4e0b\u3052\u308b<\/b>\u4e8b\u3067\u3059\u3002<\/p>\n<p>\u878d\u89e3\u5f8c\u306f\u7d30\u80de\u304cDMSO\u306b\u66dd\u3055\u308c\u308b\u6642\u9593\u3092\u3067\u304d\u308b\u3060\u3051\u6700\u5c0f\u9650\u306b\u6291\u3048\u3001\u57f9\u5730\u3078\u61f8\u6fc1\u3001\u9060\u5fc3\u306e\u5f8c\u3001\u901f\u3084\u304b\u306b\u65b0\u9bae\u306a\u57f9\u5730\u306b\u7f6e\u304d\u63db\u3048\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u518d\u61f8\u6fc1\u306f\u7a4f\u3084\u304b\u306a\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u5fc3\u639b\u3051\u3001\u57f9\u990a\u5bb9\u5668\u306b\u64ad\u7a2e\u3057\u305f\u3089\u76f4\u3061\u306b\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306b\u79fb\u3057\u3066\u57f9\u990a\u3092\u958b\u59cb\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u5f53\u5ba4\u3067\u306f\u4e00\u822c\u7684\u306a\u878d\u89e3\u65b9\u6cd5\u3068\u3057\u3066\u3001\u51cd\u7d50\u7d30\u80de\u3092\u4e88\u3081 5mL \u306e\u57f9\u5730\u306b\u61f8\u6fc1\u5f8c\u3001\u300c1000rpm\uff08200 xg \u524d\u5f8c\uff09\u30013\u5206\u3001\u5ba4\u6e29\u300d\u3067\u9060\u5fc3\u3057\u3001\u4e0a\u6e05\u3092\u53d6\u308a\u9664\u304f\u4e00\u9023\u306e\u64cd\u4f5c\u30922\u56de\u7e70\u308a\u8fd4\u3059\u69d8\u3001\u3054\u6848\u5185\u3057\u3066\u304a\u308a\u307e\u3059\u3002<br \/>\n<a href=\"\/ja\/manual\/thawing_of_frozen_ampoules\">\u9001\u4ed8\u3055\u308c\u305f\u7d30\u80de\u306e\u878d\u89e3\u30fb\u57f9\u990a\u65b9\u6cd5\u3092\u53c2\u7167<\/a><br \/>\n\u3053\u3053\u3067\u4e00\u9023\u306e\u64cd\u4f5c\u3092\u7e70\u308a\u8fd4\u3057\u884c\u3046\u306e\u306f\u3001DMSO\u306e\u6b8b\u5b58\u6fc3\u5ea6\u3092\u5341\u5206\u306b\u4e0b\u3052\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n<p>\u305f\u3060\u3057\u3001\u30d2\u30c8\u8840\u7403\u7cfb\u7d30\u80de\u306a\u3069\u3001\u878d\u89e3\u5f8c\u306e\u751f\u5b58\u7387\u304c\u6975\u7aef\u306b\u4f4e\u304f\u306a\u308b\u4e00\u90e8\u306e\u7d30\u80de\u682a\u306b\u304a\u3044\u3066\u306f\u3001\u4e00\u9023\u306e\u9060\u5fc3\u64cd\u4f5c\u306b\u3088\u3063\u3066\u8d77\u3053\u308b\u7d30\u80de\u3078\u306e\u7269\u7406\u7684\u306a\u30c0\u30e1\u30fc\u30b8\u3092\u7e70\u308a\u8fd4\u3055\u306a\u3044\u65b9\u304c\u3001\u57f9\u990a\u306e\u7acb\u3061\u4e0a\u304c\u308a\u304c\u826f\u304f\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u3053\u306e\u69d8\u306a\u5834\u5408\u306f\u51cd\u7d50\u7d30\u80de\u3092\u4e88\u308110 mL\u306e\u57f9\u5730\u306b\u61f8\u6fc1\u3057\u3001\u4e00\u9023\u306e\u9060\u5fc3\u64cd\u4f5c\u30921\u56de\u306b\u7559\u3081\u3066\u3001\u901f\u3084\u304b\u306b\u57f9\u990a\u3092\u958b\u59cb\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u8a73\u7d30\u306f\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u682a\u306e\u300c\u57f9\u990a\u958b\u59cb\u306b\u3042\u305f\u3063\u3066\u306e\u6ce8\u610f\u300d\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002\n<\/ul>\n<hr \/>\n<p><b>\u64ad\u7a2e\uff5e\u7d30\u80de\u306e\u56de\u5fa9<\/b><\/p>\n<ul>\n<li>\u4e00\u822c\u306b\u30d2\u30c8\u8840\u7403\u7cfb\u7d30\u80de\u306f\u51cd\u7d50\u30fb\u878d\u89e3\u306e\u30c0\u30e1\u30fc\u30b8\u3092\u53d7\u3051\u3084\u3059\u304f\u3001\u56de\u5fa9\u304c\u9045\u3044\u3082\u306e\u304c\u591a\u3044\u3067\u3059\u3002<\/li>\n<li>\u64ad\u7a2e\u5bc6\u5ea6\u304c\u7d30\u80de\u306e\u7acb\u3061\u4e0a\u304c\u308a\u306b\u5927\u304d\u304f\u5f71\u97ff\u3057\u307e\u3059\u3002<\/li>\n<p>\u203b\u5f53\u5ba4\u306e\u7d30\u80de\u306f\u901a\u5e38\u3001\u03c660mm dish 2\u679a \u53c8\u306fT25 cm2 flask 2\u672c\u3078\u306e\u64ad\u7a2e\u3092\u304a\u9858\u3044\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u6d6e\u904a\u7d30\u80de\u306e\u5834\u5408\u306f T25 cm2 flask \u306e\u4f7f\u7528\u3092\u63a8\u5968\u3057\u307e\u3059\u3002<br \/>\n\u3000\u63a8\u5968\u3055\u308c\u308b\u64ad\u7a2e\u5bc6\u5ea6\u306f\u7d30\u80de\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u304c\u3001\u4e00\u822c\u7684\u306b\u878d\u89e3\u76f4\u5f8c\u306f\u751f\u5b58\u7387\u304c\u4f4e\u304f\u306a\u308a\u3084\u3059\u3044\u305f\u3081\u3001\u6642\u306b\u306f\u8996\u91ce\u3092\u8986\u3046\u304f\u3089\u3044\u3001\u901a\u5e38\u3088\u308a\u5bc6\u5ea6\u3092\u9ad8\u304f\u3059\u308b\u3053\u3068\u3082\u6709\u52b9\u306a\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n<li>\u878d\u89e3\u7fcc\u65e5\u306f\u751f\u5b58\u7387\u304c\u5927\u304d\u304f\u4f4e\u4e0b\u3059\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<p>\u203b\u7279\u306b\u521d\u671f\u306f\u591a\u304f\u306e\u6b7b\u7d30\u80de\u304c\u89b3\u5bdf\u3055\u308c\u307e\u3059\u3002\u307e\u305f\u3001\u751f\u304d\u6b8b\u3063\u305f\u7d30\u80de\u306e\u4e2d\u306b\u3082\u3044\u3073\u3064\u306a\u5f62\u3084\u840e\u7e2e\u3057\u3066\u3044\u308b\u3082\u306e\u304c\u542b\u307e\u308c\u3066\u3044\u3066\u3001\u3053\u308c\u3089\u3082\u307e\u305f\u5909\u6027\u3057\u3066\u6b7b\u3093\u3067\u3044\u304d\u307e\u3059\u3002<br \/>\n\u3000\u307b\u3068\u3093\u3069\u306e\u5834\u5408 \u6570\u65e5\u7a0b\u5ea6\u3067\u56de\u5fa9\u3057\u3066\u304d\u307e\u3059\u304c\u3001\u56de\u5fa9\u307e\u3067\u306b\u5fc5\u8981\u306a\u65e5\u6570\u306f\u878d\u89e3\u64cd\u4f5c\u3067\u7d30\u80de\u304c\u53d7\u3051\u305f\u30c0\u30e1\u30fc\u30b8\u306e\u5f71\u97ff\u306b\u3088\u3063\u3066\u5909\u52d5\u3057\u307e\u3059\u3002<br \/>\n\u751f\u304d\u6b8b\u3063\u305f\u7d30\u80de\u304c\u56de\u5fa9\u3057\u5897\u6b96\u3092\u59cb\u3081\u308b\u3068\u3001\u8f2a\u90ed\u304c\u306f\u3063\u304d\u308a\u3057\u305f\u7403\u5f62\u306b\u8fd1\u3044\u7d30\u80de\u306e\u5272\u5408\u304c\u5f90\u3005\u306b\u5897\u3048\u3066\u3044\u304d\u307e\u3059\u3002<br \/>\n\u3000\u521d\u671f\u306b\u591a\u304f\u89b3\u5bdf\u3055\u308c\u305f\u6b7b\u7d30\u80de\u306f\u5f90\u3005\u306b\u5f62\u614b\u304c\u5d29\u308c\u3001\u57f9\u5730\u306b\u6eb6\u3051\u3066\u5206\u6563\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n<li>\u7d30\u80de\u304c\u51cd\u7d50\u878d\u89e3\u306e\u30c0\u30e1\u30fc\u30b8\u304b\u3089\u56de\u5fa9\u3059\u308b\u307e\u3067\u306f\u3001\u3042\u307e\u308a<b>\u300c\u624b\u3092\u52a0\u3048\u306a\u3044\u300d<\/b>\u65b9\u304c\u826f\u3044\u7d50\u679c\u3068\u306a\u308b\u5834\u5408\u304c\u591a\u3044\u3067\u3059\u3002<\/li>\n<p>\u203b\u300c\u624b\u3092\u52a0\u3048\u306a\u3044\u300d\u3068\u306f\u3001\u4f8b\u3048\u3070\u56de\u53ce\u3001\u9060\u5fc3\u3001\u57f9\u5730\u4ea4\u63db\u3001\u7d99\u4ee3\u7b49\u306e\u64cd\u4f5c\u306f\u7d30\u80de\u3078\u306e\u7269\u7406\u7684\u306a\u30c0\u30e1\u30fc\u30b8\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002<br \/>\n\u307e\u305f\u3001\u57f9\u990a\u74b0\u5883\u3092\u5909\u3048\u308b\u3088\u3046\u306a\u64cd\u4f5c\uff08\u57f9\u990a\u7d30\u80de\u306e\u4ee3\u8b1d\u306b\u3088\u3063\u3066\u751f\u3058\u305f\u99b4\u5316\u57f9\u5730\u304c\u9664\u53bb\u3055\u308c\u308b \u3042\u308b\u3044\u306f\u5e0c\u91c8\u3055\u308c\u308b\u7b49\uff09\u3082\u5897\u6b96\u4e0d\u826f\u306e\u4e00\u56e0\u3068\u306a\u308a\u307e\u3059\u3002<br \/>\n\u56de\u5fa9\u307e\u3067\u306f\u5c11\u91cf\u306e\u65b0\u9bae\u57f9\u5730\u3092\u8db3\u3059\u7a0b\u5ea6\u306b\u7559\u3081\u3001\u7d99\u4ee3\u53ef\u80fd\u306a\u5bc6\u5ea6\u307e\u3067\u5145\u5206\u306b\u5897\u6b96\u3059\u308b\u306e\u3092\u5f85\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<li>\u57f9\u990a\u6db2\u306b\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u3092\u6dfb\u52a0\u3057\u3066\u3044\u308b\u7d30\u80de\u682a\u306f\u3001\u6642\u9593\u3068\u5171\u306b\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u304c\u6d88\u8cbb\u3055\u308c\u307e\u3059\u3002<\/li>\n<p>\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u9069\u5b9c\u6dfb\u52a0\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\u203b\u6dfb\u52a0\u306e\u76ee\u5b89\u306f3\uff5e4\u65e5\u3054\u3068\u3001\u3082\u3057\u304f\u306f\u57f9\u5730\u4ea4\u63db\u7b49\u306e\u64cd\u4f5c\u6642\u306b\u884c\u306a\u3044\u307e\u3059\u3002\u6dfb\u52a0\u91cf\u306f\u57f9\u5730\u7dcf\u91cf\u304b\u3089\u8a08\u7b97\u3057\u3066\u6dfb\u52a0\u3057\u3066\u304f\u3060\u3055\u3044\u3002<br \/>\n\uff08\u57f9\u5730\u7dcf\u91cf\uff1d\u57f9\u990a\u5bb9\u5668\u5185\u306e\u57f9\u5730\u91cf + \u65b0\u3057\u304f\u8db3\u3057\u305f\u57f9\u5730\u91cf\uff09<br \/>\n\u3000<\/ul>\n<hr \/>\n<p><b>\u7d99\u4ee3<\/b><\/p>\n<ul>\n<li>\u6d3b\u767a\u306a\u5897\u6b96\u50cf\u304c\u89b3\u5bdf\u3055\u308c\u3001\u5149\u6ca2\u306e\u5f37\u3044\u5897\u6b96\u7d30\u80de\u304c\u6570\u3092\u5897\u3057\u3066\u8996\u91ce\u3092\u57cb\u3081\u3066\u304f\u308c\u3070\u3001\u6700\u521d\u306e\u7d99\u4ee3\u306e\u30bf\u30a4\u30df\u30f3\u30b0 \u3067\u3059\u3002<\/li>\n<li>\u7d99\u4ee3\u306f\u9060\u5fc3\u306b\u3088\u3063\u3066\u57f9\u5730\u3092\u5b8c\u5168\u306b\u7f6e\u304d\u63db\u3048\u308b\u3088\u308a\u3082\u3001\u7d30\u80de\u3092\u305d\u306e\u307e\u307e\u99b4\u5316\u57f9\u5730\u3068\u4e00\u7dd2\u306b\u5e0c\u91c8\u3057\u3066\u884c\u3063\u305f\u65b9\u304c\u826f\u3044\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u57f9\u5730\u306b\u6d6e\u904a\u3057\u3066\u3044\u308b\u6b7b\u7d30\u80de\u306f\u591a\u304f\u306e\u5834\u5408\u3001\u7d99\u4ee3\u3068\u5171\u306b\u5f90\u3005\u306b\u305d\u306e\u5272\u5408\u304c\u6e1b\u5c11\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/li>\n<li>\u6700\u521d\u306e\u7d99\u4ee3\u306f\u3042\u307e\u308a\u7d30\u80de\u5bc6\u5ea6\u3092\u4f4e\u304f\u305b\u305a\u3001\u5e0c\u91c8\u7387\u3092\u6291\u3048\u3066\u884c\u3046\u3068\u826f\u3044\u3067\u3057\u3087\u3046\u3002<br \/>\n\uff08\u4f8b\u3048\u30701\uff1a2\uff09<br \/>\n\u5897\u6b96\u304c\u5b89\u5b9a\u3057\u3066\u304f\u308c\u3070\u3001\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u306b\u63a8\u5968\u3055\u308c\u3066\u3044\u308b\u5e0c\u91c8\u7387\u3067\u306e\u7d99\u4ee3\u304c\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<li>\u5b89\u5b9a\u3057\u3066\u5897\u6b96\u3059\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u3089\u3001\u4eca\u5ea6\u306f\u30aa\u30fc\u30d0\u30fc\u30b0\u30ed\u30fc\u30b9\uff08\u5bc6\u5ea6\u904e\u5270\uff09\u306b\u3082\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/li>\n<p>\u5bc6\u5ea6\u904e\u5270\u306b\u306a\u308b\u3053\u3068\u306f\u3001\u7d30\u80de\u306e\u751f\u5b58\u3084\u5897\u6b96\u306b\u5fc5\u8981\u306a\u6210\u5206\u304c\u76f8\u5bfe\u7684\u306b\u4e0d\u8db3\u3059\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u3001\u5373\u3061\u3001\u7d30\u80de\u6b7b\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002\u5897\u6b96\u304c\u304d\u308f\u3081\u3066\u901f\u3044\u6d6e\u904a\u7d30\u80de\u3067\u306f\u3001\u30aa\u30fc\u30d0\u30fc\u30b0\u30ed\u30fc\u30b9\u306b\u3088\u3063\u3066\u7d30\u80de\u304c\u5168\u6ec5\u3059\u308b\u30b1\u30fc\u30b9\u306e\u5831\u544a\u3082\u591a\u3044\u3067\u3059\u3002\n<\/p><\/ul>\n","protected":false},"excerpt":{"rendered":"<p>PDF\u7248\u306f\u3053\u3061\u3089 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