{"id":11469,"date":"2018-10-23T22:46:36","date_gmt":"2018-10-23T13:46:36","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/ja\/?page_id=11469"},"modified":"2026-04-20T15:24:25","modified_gmt":"2026-04-20T06:24:25","slug":"dna_barcoding","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/announcement\/dna_barcoding","title":{"rendered":"\u65b0\u3057\u3044\u52d5\u7269\u7a2e\u540c\u5b9a\u691c\u67fb\u306e\u5c0e\u5165\u3068\u89e3\u6790\u7d50\u679c\u306b\u3064\u3044\u3066"},"content":{"rendered":"

\uff1c\u7d30\u80de\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u65b0\u898f\u691c\u67fb\u306e\u5c0e\u5165\u3068\u305d\u306e\u7d50\u679c\u306e\u304a\u77e5\u3089\u305b\uff1e<\/p>\n

\u3000\u7406\u7814\u7d30\u80de\u30d0\u30f3\u30af\u4e8b\u696d\u958b\u59cb\u4ee5\u6765\u3001\u5bc4\u8a17\u7d30\u80de\u682a\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u691c\u67fb\u6cd5\u3068\u3057\u3066\u306f\u3001\uff12\u7a2e\u985e\u306e\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\uff08lactate dehydrogenase\u53ca\u3073nucleotide phosphorylase\uff09\u3092\u89e3\u6790\u3059\u308b\u691c\u67fb\u6cd5\u3092\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u3053\u308c\u306f\u3001\u591a\u7a2e\u985e\u306e\u52d5\u7269\u304c\u5171\u901a\u3057\u3066\u6301\u3063\u3066\u3044\u308b\u540c\u3058\u9175\u7d20\u3067\u3082\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u69cb\u9020\u304c\u7570\u306a\u308a\u3001\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u3063\u3066\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3057\u3066\u3001\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u751f\u5316\u5b66\u7684\u306a\u691c\u67fb\u6cd5\u3067\u3059\u3002\u3057\u304b\u3057\u30012015\u5e74\u306b\u3053\u306e\u30a2\u30a4\u30bd\u30b6\u30a4\u30e0\u691c\u67fb\u3067\u306f\u4e0d\u5341\u5206\u306a\u7d30\u80de\u682a\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u5224\u660e\u3057\u307e\u3057\u305f\u3002
\nhttps:\/\/cell.brc.riken.jp\/ja\/23cln<\/a><\/p>\n

\u3000\u305d\u3053\u3067\u3001\u3088\u308a\u7cbe\u5ea6\u304c\u9ad8\u3044\u5206\u5b50\u751f\u7269\u5b66\u7684\u306a\u691c\u67fb\u3068\u3057\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u907a\u4f1d\u5b50\u3092\u5bfe\u8c61\u3068\u3057\u305f\u7a2e\u306e\u540c\u5b9a\u6cd5\uff08\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u907a\u4f1d\u5b50PCR\u540c\u5b9a\u691c\u67fb\uff09\u3092\u5c0e\u5165\u3057\u30012016\u5e74\u307e\u3067\u306b\u540c\u691c\u67fb\u3092\u7d42\u4e86\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u691c\u67fb\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\uff13\u7a2e\u985e\u306e\u907a\u4f1d\u5b5012S ribosomal RNA, 16S ribosomal RNA\u3082\u3057\u304f\u306fcytochrome b\u306e\u5869\u57fa\u914d\u5217\u304c\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u3053\u3068\u3092\u5229\u7528\u3059\u308b\u3082\u306e\u3067\u3042\u308a\u3001\u52d5\u7269\u7a2e\u7279\u7570\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3057\u3066\u3001PCR\u7523\u7269\u306e\u6709\u7121\u3067\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\uff08\u6587\u732e\uff11<\/span>\u3001\u56f3\uff11<\/a>\uff09\uff08DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u306b\u3088\u308b\u5869\u57fa\u914d\u5217\u306b\u57fa\u3065\u304f\u691c\u67fb\u3067\u306f\u3042\u308a\u307e\u305b\u3093\uff09\u3002\u3057\u304b\u3057\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u907a\u4f1d\u5b50PCR\u540c\u5b9a\u691c\u67fb\u306f\u3001\u7d30\u80de\u30d0\u30f3\u30af\u3078\u306e\u5bc4\u8a17\u304c\u6bd4\u8f03\u7684\u591a\u3044\u53c8\u306f\u5229\u7528\u8005\u6570\u304c\u591a\u304414\u7a2e\u985e\u306e\u52d5\u7269\u306b\u95a2\u3057\u3066\u958b\u767a\u3055\u308c\u305f\u3082\u306e\u3067\u3042\u308a\uff08\u6587\u732e\uff11<\/span>\uff09\u3001\u4ed6\u306e26\u7a2e\u306e\u52d5\u7269\u306b\u7531\u6765\u3059\u308b61\u682a\u306e\u7d30\u80de\u306b\u306f\u9069\u7528\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002
\n\u3000\u540c\u5b9a\u3067\u304d\u308b\u7531\u6765\u52d5\u7269\u7a2e\u3092\u62e1\u5927\u3059\u308b\u305f\u3081\u306e\u691c\u67fb\u6cd5\u3068\u3057\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306ecytochrome c oxidase subunit 1 (COI) \u907a\u4f1d\u5b50\uff08\u56f3\uff11<\/a>\u306b\u304a\u3044\u3066\u7dd1\u8272\u306e\u67a0\u3092\u4ed8\u3051\u3066\u3042\u308b\u907a\u4f1d\u5b50\uff09\u306e\u7d04600bp\u3092PCR\u5897\u5e45\u3057\u3001\u305d\u306ePCR\u7523\u7269\u306eDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u884c\u3046\u3053\u3068\u3067\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u65b9\u6cd5\u304c\u958b\u767a\u3055\u308c\u307e\u3057\u305f\uff08COI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\uff09\u3002\u540c\u691c\u67fb\u306f\u3001COI\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7d50\u679c\u304cNational Center for Biotechnology Information\uff08NCBI\uff1a\u7c73\u56fd\u7acb\u751f\u7269\u5de5\u5b66\u60c5\u5831\u30bb\u30f3\u30bf\u30fc\uff09\u7b49\u306e\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306b\u767b\u9332\u3055\u308c\u3066\u3044\u308b\u5404\u7a2e\u52d5\u7269\u306eCOI\u907a\u4f1d\u5b50\u914d\u5217\u306e\u3069\u308c\u3068\u4e00\u81f4\u3059\u308b\u304b\u306b\u3088\u3063\u3066\u3001\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\uff08\u6587\u732e\uff12<\/span>\uff09\u3002\u3053\u308c\u306f\u3001DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u6280\u8853\u306e\u9032\u6b69\u306b\u4f34\u3063\u3066\u3001\u69d8\u3005\u306a\u52d5\u7269\u7a2e\u3067COI\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u304c\u5b9f\u65bd\u3055\u308c\u3001\u305d\u306e\u89e3\u6790\u7d50\u679c\u304c\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306b\u767b\u9332\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u305f\u3081\u306b\u3001\u52d5\u7269\u7a2e\u540c\u5b9a\u3078\u306e\u5fdc\u7528\u304c\u53ef\u80fd\u3068\u306a\u3063\u305f\u691c\u67fb\u65b9\u6cd5\u3067\u3059\u3002\u305d\u3053\u3067\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u907a\u4f1d\u5b50PCR\u540c\u5b9a\u691c\u67fb\u3067\u306f\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3067\u304d\u306a\u304b\u3063\u305f\u52d5\u7269\u7d30\u80de\u306b\u3064\u3044\u3066\u30012017\u5e74\u3088\u308aCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\u3092\u5c0e\u5165\u3057\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u30013\u7a2e\u306b\u7531\u6765\u3059\u308b8\u682a\u306b\u3064\u3044\u3066\u306f\u3001NCBI \u7b49\u3067\u516c\u958b\u3055\u308c\u3066\u3044\u308bCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306e\u60c5\u5831\u304c\u4e0d\u5341\u5206\u3067\u3042\u308a\u3001COI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\u3067\u3082\u7531\u6765\u52d5\u7269\u7a2e\u306e\u540c\u5b9a\u304c\u4e0d\u53ef\u80fd\u3067\u3057\u305f\u3002\u305d\u306e\u3088\u3046\u306a\u30b1\u30fc\u30b9\u3067\u306f\u4ed6\u306e\u907a\u4f1d\u5b50\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u3092\u691c\u8a0e\u3057\u3001\u4eca\u56de\u306f\u9069\u7528\u53ef\u80fd\u306acytochrome b\u907a\u4f1d\u5b50\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002
\n\u3000\u89e3\u6790\u306e\u7d50\u679c\u300161\u682a\u4e2d57\u682a\u306b\u3064\u3044\u3066\u306f\u5bc4\u8a17\u8005\u306e\u767b\u9332\u60c5\u5831\u3068\u4e00\u81f4\u3057\u3066\u3044\u307e\u3057\u305f\uff08\u8868\uff11<\/a>\uff09\u3002\u3057\u304b\u3057\u3001\u4e0b\u8a18\u306e4\u682a\u306b\u3064\u3044\u3066\u306f\u7531\u6765\u52d5\u7269\u7a2e\u304c\u5bc4\u8a17\u8005\u306e\u767b\u9332\u60c5\u5831\u3068\u306f\u7570\u306a\u308b\u7d50\u679c\u3092\u5f97\u307e\u3057\u305f\u3002<\/p>\n

\u2460 \u3010\u30c6\u30a3\u30e9\u30d4\u30a2\u7531\u6765\u7d30\u80de\u306eHepa-T1\u7d30\u80de\u3011\uff08\u56f3\uff12<\/a>\uff09
\n\u3000Hepa-T1\u7d30\u80de\uff08RCB1156\uff09\u306f1995\u5e743\u6708\u306b\u56fd\u5916\u306e\u7814\u7a76\u8005\u304b\u3089\u30c6\u30a3\u30e9\u30d4\u30a2\u7531\u6765\u7d30\u80de\u682a\u3068\u3057\u3066\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u3067\u3059\u3002
\n\u3000\u56f3\uff12<\/span>\u306b\u793a\u3057\u307e\u3057\u305f\u3088\u3046\u306b\u3001Hepa-T1\u7d30\u80de\u304b\u3089\u5f97\u3089\u308c\u305fCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2461<\/span>\u306f\u3001NCBI\u306b\u767b\u9332\u3055\u308c\u3066\u3044\u308b\u30c6\u30a3\u30e9\u30d4\u30a2COI\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2460<\/span>\u3068\u306f\u5927\u304d\u304f\u7570\u306a\u308a\uff08\u4e00\u81f4\u738779.8\uff05\uff09\u3001NCBI\u306b\u767b\u9332\u3055\u308c\u3066\u3044\u308b\u30a6\u30ca\u30aeCOI\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2464<\/span>\u53ca\u3073\u30a6\u30ca\u30ae\u7531\u6765\u306eHepa-E1\u7d30\u80de\uff08RCB1155\uff09\u304b\u3089\u5f97\u3089\u308c\u305fCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2463<\/span>\u3068\u306f99.8\uff05\u4e00\u81f4\u3057\u3066\u3044\u307e\u3057\u305f\u3002
\n\u3000\u5bc4\u8a17\u76f4\u5f8c\u306e\u7d30\u80de\u2462<\/span>\u3067\u3082\u540c\u3058\u7d50\u679c\u3067\u3057\u305f\u306e\u3067\uff08\u56f3\uff12<\/a>\uff09\u3001Hepa-T1\u7d30\u80de\u306f\u5bc4\u8a17\u5f53\u521d\u304b\u3089\u30a6\u30ca\u30ae\u7531\u6765\u306e\u7d30\u80de\u682a\u3067\u3042\u3063\u305f\u3068\u5224\u65ad\u3055\u308c\u307e\u3059\u3002
\n\u3000\u5c1a\u3001Hepa-T1\u7d30\u80de\u3068Hepa-E1\u7d30\u80de\u306f\u524d\u8ff0\u306e\u56fd\u5916\u306e\u540c\u3058\u7814\u7a76\u8005\u304b\u3089\u540c\u6642\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u3067\u3042\u308a\u3001\u5bc4\u8a17\u8005\u81ea\u8eab\u3082\u6c17\u304c\u4ed8\u304b\u306a\u3044\u3046\u3061\u306bHepa-T1\u7d30\u80de\u304cHepa-E1\u7d30\u80de\u306b\u3088\u3063\u3066\u7f6e\u63db\u3055\u308c\u305f\u30b1\u30fc\u30b9\u3067\u3042\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3082\u306e\u3068\u8003\u3048\u307e\u3059\u3002\u3053\u3046\u3057\u305f\u7d30\u80de\u306e\u7f6e\u63db\uff08\u53d6\u308a\u9055\u3048\u7b49\uff09\u306e\u539f\u56e0\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u6587\u732e\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\uff08\u6587\u732e\uff13<\/span>\uff09\u3002<\/p>\n

\u2461 \u3010\u30af\u30ef\u30b4\u30de\u30c0\u30e9\u30d2\u30c8\u30ea\u7531\u6765\u7d30\u80de\u306eFRI-SpIm-1229\u7d30\u80de\u3011\uff08\u56f3\uff13<\/a>\uff09
\n\u3000FRI-SpIm-1229\u7d30\u80de\uff08RCB0281\uff09\u306f2000\u5e7410\u6708\u306b\u56fd\u5185\u306e\u7814\u7a76\u8005\u304b\u3089\u30af\u30ef\u30b4\u30de\u30c0\u30e9\u30d2\u30c8\u30ea\uff08\u86fe\u306e\u4e00\u7a2e\uff09\u7531\u6765\u7d30\u80de\u682a\u3068\u3057\u3066\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u3067\u3059\u3002
\n\u3000\u30af\u30ef\u30b4\u30de\u30c0\u30e9\u30d2\u30c8\u30ea\u306eCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306f\u516c\u958b\u3055\u308c\u3066\u3044\u308b\u60c5\u5831\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001\u56f3\uff13<\/span>\u306b\u793a\u3057\u307e\u3059\u3088\u3046\u306b\u3001FRI-SpIm-1229\u7d30\u80de\u304b\u3089\u5f97\u3089\u308c\u305fCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2460<\/span>\u306f\u3001\u30e8\u30c8\u30a6\u30ac\uff08\u86fe\u306e\u4e00\u7a2e\uff09\u306eCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2462<\/span>\u306899.5\uff05\u4e00\u81f4\u3057\u3066\u3044\u307e\u3057\u305f\u3002
\n\u3000\u5bc4\u8a17\u76f4\u5f8c\u306e\u7d30\u80de\u2461<\/span>\u3067\u308299.2\uff05\u306e\u4e00\u81f4\u3067\u3057\u305f\u306e\u3067\uff08\u56f3\uff13<\/a>\uff09\u3001FRI-SpIm-1229\u7d30\u80de\u306f\u5bc4\u8a17\u5f53\u521d\u304b\u3089\u30e8\u30c8\u30a6\u30ac\u7531\u6765\u306e\u7d30\u80de\u682a\u3067\u3042\u3063\u305f\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3068\u63a8\u6e2c\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u2462 \u3010\u30ab\u30a8\u30eb\u7531\u6765\u7d30\u80de\u306eLAH1, LAH2\u7d30\u80de\u3011\uff08\u56f3\uff14<\/a>\u3001\u56f35<\/a>\u3001\u56f3\uff16<\/a>\uff09
\n\u3000\u30ab\u30a8\u30eb\u7531\u6765\u306eLAH1 (RCB1733)\u53ca\u3073LAH2 (RCB1735)\u7d30\u80de\u306f\u3001\u540c\u3058\u304f\u30ab\u30a8\u30eb\u7531\u6765\u306eLAH3 (RCB1734) \u7d30\u80de\u3068\u540c\u6642\u306b\u30012001\u5e743\u6708\u306b\u56fd\u5185\u306e\u540c\u3058\u7814\u7a76\u8005\u304b\u3089\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u3067\u3059\u3002
\n\u3000\u30ab\u30a8\u30eb\u306b\u3064\u304d\u307e\u3057\u3066\u306fCOI\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u767b\u9332\u304c\u4e0d\u5341\u5206\u3067\u3042\u3063\u305f\u305f\u3081\u306bCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\u3067\u306f\u5224\u5225\u304c\u4e0d\u53ef\u80fd\u3067\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306ecytochrome b\u907a\u4f1d\u5b50\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u30c7\u30fc\u30bf\u304cNCBI\u306b\u767b\u9332\u3055\u308c\u3066\u304a\u308a\u3001cytochrome b\u907a\u4f1d\u5b50\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u306b\u3066\u691c\u67fb\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u306e\u3067\uff08\u6587\u732e\uff14<\/span>\uff09\u3001LAH1, LAH2, LAH3\u7d30\u80de\u306b\u3064\u304d\u307e\u3057\u3066\u540c\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<\/p>\n

LAH1\u7d30\u80de\uff08\u56f3\uff15<\/a>\u306e\u2461\u2462<\/span>\uff09
\n\u5bc4\u8a17\u8005\u767b\u9332\u60c5\u5831\uff1a\u91ce\u751f\u578b\u30fb\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\uff08\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u7a2e\uff09\u7531\u6765
\nLAH1\u7d30\u80de\u306ecytochrome b\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2461<\/span>\u306f\u3001\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u7a2e\uff08\u56f3\uff15<\/a>\u306e\u2460<\/span>\uff09\u3067\u306f\u306a\u304f\uff08\u4e00\u81f4\u7387\uff1a74.5\uff05\uff09\u3001\u30d2\u30ad\u30ac\u30a8\u30eb\u5c5e\u30cb\u30db\u30f3\u30d2\u30ad\u30ac\u30a8\u30eb\u7a2e\u30a2\u30ba\u30de\u30d2\u30ad\u30ac\u30a8\u30eb\u4e9c\u7a2e\uff08\u56f3\uff15<\/a>\u306e\u2463<\/span>\uff09\u3068\u6700\u3082\u9ad8\u3044\u76f8\u540c\u6027\u3092\u793a\u3057\u307e\u3057\u305f\uff0899.5\uff05\u306e\u4e00\u81f4\uff09<\/p>\n

LAH2\u7d30\u80de\uff08\u56f3\uff16<\/a>\u306e\u2461\u2462\uff09
\n\u5bc4\u8a17\u8005\u767b\u9332\u60c5\u5831\uff1a\u30a2\u30eb\u30d3\u30ce\u30fb\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\uff08\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u7a2e\uff09\u7531\u6765
\nLAH2\u7d30\u80de\u306ecytochrome b\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2461<\/span>\u306f\u3001\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u7a2e\uff08\u56f3\uff16<\/a>\u306e\u2460\uff09\u3067\u306f\u306a\u304f\uff08\u4e00\u81f4\u7387\uff1a90.5\uff05\uff09\u3001\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u7a2e\u30ca\u30b4\u30e4\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u4e9c\u7a2e\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\uff08\u56f3\uff16<\/a>\u306e\u2465\uff09\u3068100\uff05\u4e00\u81f4\u3057\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n

\u3000\u4e0a\u8a18\u306e\u3068\u304a\u308a\u3001LAH1\u7d30\u80de\u3068LAH2\u7d30\u80de\u306f\u5bc4\u8a17\u8005\u306e\u767b\u9332\u60c5\u5831\u3068\u306f\u7570\u306a\u308b\u30ab\u30a8\u30eb\u7531\u6765\u3067\u3042\u308b\u3068\u3044\u3046\u7d50\u679c\u3067\u3057\u305f\u3002<\/p>\n

\u3000\u56f3\uff15\u2462<\/span>\u3001\u56f3\uff16\u2462\u2464<\/span>\u306b\u3082\u793a\u3057\u3066\u3042\u308a\u307e\u3059\u3068\u304a\u308a\u3001\u5bc4\u8a17\u76f4\u5f8c\u306e\u7d30\u80de\u3067\u3082\u5168\u3066\u540c\u3058\u7d50\u679c\u3067\u3057\u305f\u306e\u3067\u3001LAH1\u7d30\u80de\u306f\u5bc4\u8a17\u5f53\u521d\u304b\u3089\u30d2\u30ad\u30ac\u30a8\u30eb\u5c5e\u30cb\u30db\u30f3\u30d2\u30ad\u30ac\u30a8\u30eb\u7a2e\u30a2\u30ba\u30de\u30d2\u30ad\u30ac\u30a8\u30eb\u4e9c\u7a2e\u3001\u307e\u305f\u3001LAH2\u7d30\u80de\u306f\u5bc4\u8a17\u5f53\u521d\u304b\u3089\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u7a2e\u30ca\u30b4\u30e4\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u4e9c\u7a2e\u3067\u3042\u3063\u305f\u3068\u5224\u65ad\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u3000\u4e00\u65b9\u3067\u3001LAH3\u7d30\u80de\u306f\u3001\u4e0b\u8a18\u306e\u3068\u304a\u308a\u5bc4\u8a17\u8005\u306e\u767b\u9332\u60c5\u5831\u3068\u4e00\u81f4\u3057\u3066\u3044\u307e\u3057\u305f\u3002<\/p>\n

LAH3\u7d30\u80de\uff08\u56f3\uff16<\/a>\u306e\u2463\u2464\uff09
\n\u5bc4\u8a17\u8005\u767b\u9332\u60c5\u5831\uff1a\u30a2\u30eb\u30d3\u30ce\u30fb\u30ca\u30b4\u30e4\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\uff08\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u7a2e\u30ca\u30b4\u30e4\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u4e9c\u7a2e\uff09\u7531\u6765
\nLAH3\u7d30\u80de\u306ecytochrome b\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u2463\u306f\u3001\u30c8\u30ce\u30b5\u30de\u30ac\u30a8\u30eb\u5c5e\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u7a2e\u30ca\u30b4\u30e4\u30c0\u30eb\u30de\u30ac\u30a8\u30eb\u4e9c\u7a2e\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\uff08\u56f3\uff16<\/a>\u306e\u2465\uff09\u3068100\uff05\u4e00\u81f4<\/p>\n

\u3000LAH2\u7d30\u80de\u306fLAH3\u7d30\u80de\u3068\u540c\u3058\u7d50\u679c\u3067\u3057\u305f\u3002\u524d\u8ff0\u306e\u3088\u3046\u306b\u3001\u3053\u308c\u3089\uff13\u682a\u306f\u540c\u3058\u7814\u7a76\u8005\u304b\u3089\u540c\u6642\u306b\u5bc4\u8a17\u3092\u53d7\u3051\u305f\u7d30\u80de\u682a\u3067\u3042\u308a\u3001\u5bc4\u8a17\u8005\u81ea\u8eab\u3082\u6c17\u304c\u4ed8\u304b\u306a\u3044\u3046\u3061\u306bLAH2\u7d30\u80de\u306fLAH3\u7d30\u80de\u306b\u3088\u3063\u3066\u7f6e\u63db\u3055\u308c\u305f\u30b1\u30fc\u30b9\u3067\u3042\u308b\u53ef\u80fd\u6027\u3092\u5426\u5b9a\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u3000\u4ee5\u4e0a\u306e\u3088\u3046\u306b\u30014\u682a\u306e\u7531\u6765\u52d5\u7269\u7a2e\u304c\u5bc4\u8a17\u8005\u60c5\u5831\u3068\u7570\u306a\u308b\u3053\u3068\u304c\u5224\u660e\u3044\u305f\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u307e\u3067\u767a\u4fe1\u3057\u3066\u304d\u305f\u60c5\u5831\u3068\u7570\u306a\u308b\u7d50\u679c\u3068\u306a\u308a\u307e\u3057\u305f\u3053\u3068\u3001\u304a\u8a6b\u3073\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002\u306a\u304a\u3001\u3053\u308c\u30894\u682a\u3092\u63d0\u4f9b\u3057\u305f\u5168\u3066\u306e\u5229\u7528\u8005\u306b\u306f\u3001\u9673\u8b1d\u3068\u8aac\u660e\u306e\u3054\u9023\u7d61\u3092\u5dee\u3057\u4e0a\u3052\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u3010\u4eca\u5f8c\u306e\u5bfe\u5fdc\u306b\u3064\u304d\u307e\u3057\u3066\u3011
\n\u3000\u65e2\u306b\u63d0\u4f9b\u3057\u3066\u3044\u308b\u7d30\u80de\u306e\u7531\u6765\u52d5\u7269\u7a2e\u306e\u540c\u5b9a\u691c\u67fb\u306f\u7d42\u4e86\u3057\u307e\u3057\u305f\u3002\u30d2\u30c8\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u500b\u4eba\u8b58\u5225\u691c\u67fb\u3067\u3042\u308bSTR\u691c\u67fb\uff08\u6587\u732e\uff13<\/span>\uff09\u3092\u3001\u30de\u30a6\u30b9\u7d30\u80de\u306b\u3064\u3044\u3066\u306f\u7cfb\u7d71\u8b58\u5225\u691c\u67fb\u3067\u3042\u308bSSLP\u691c\u67fb\uff08\u6587\u732e\uff15<\/span>\uff09\u3092\u30eb\u30fc\u30c1\u30f3\u691c\u67fb\u3068\u3057\u3066\u5b9f\u65bd\u3057\u3066\u304a\u308a\u3001\u3053\u308c\u3089\u306f\u52d5\u7269\u7a2e\u306e\u540c\u5b9a\u3082\u517c\u306d\u305f\u691c\u67fb\u306b\u306a\u3063\u3066\u3044\u307e\u3059\uff08\u7a2e\u304c\u7570\u306a\u308b\u3068\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u305b\u3093\uff09\u3002\u4eca\u5f8c\u306e\u30d2\u30c8\u3001\u30de\u30a6\u30b9\u4ee5\u5916\u306e\u52d5\u7269\u7a2e\u306e\u5bc4\u8a17\u7d30\u80de\u306b\u3064\u304d\u307e\u3057\u3066\u306f\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u907a\u4f1d\u5b50PCR\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3001\u540c\u6cd5\u3067\u5224\u5225\u3067\u304d\u306a\u3044\u52d5\u7269\u7a2e\u306b\u3064\u3044\u3066\u306fCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\u3092\u884c\u3044\u3001\u66f4\u306bCOI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\u3067\u3082\u5224\u5225\u3067\u304d\u306a\u3044\u52d5\u7269\u7a2e\u306b\u3064\u3044\u3066\u306f\u3001\u65e5\u3005\u30c7\u30fc\u30bf\u304c\u66f4\u65b0\u3055\u308c\u308bNCBI\u7b49\u306e\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3092\u8abf\u67fb\u3057\u306a\u304c\u3089\u3001cytochrome b\u907a\u4f1d\u5b50\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u7b49\u306e\u8a72\u5f53\u52d5\u7269\u7a2e\u306b\u7279\u7570\u7684\u306a\u691c\u67fb\u3092\u691c\u8a0e\u3057\u3066\u3001\u52d5\u7269\u7a2e\u306e\u540c\u5b9a\u306b\u52aa\u3081\u3066\u3044\u304d\u305f\u3044\u3068\u8003\u3048\u3066\u304a\u308a\u307e\u3059\u3002
\n\u3000\u751f\u7269\u79d1\u5b66\u5206\u91ce\u306f\u65e5\u9032\u6708\u6b69\u306e\u767a\u5c55\u3092\u9042\u3052\u3066\u304a\u308a\u3001COI\u907a\u4f1d\u5b50\u306a\u3089\u3073\u306bcytochrome b\u907a\u4f1d\u5b50\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u691c\u67fb\u304c\u53ef\u80fd\u3068\u306a\u3063\u305f\u306e\u3082\u3001\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u8aad\u6280\u8853\u306e\u9032\u6b69\u306b\u3088\u3063\u3066\u4f4e\u30b3\u30b9\u30c8\u5316\u304c\u56f3\u3089\u308c\u3001\u69d8\u3005\u306a\u52d5\u7269\u7a2e\u306e\u30b2\u30ce\u30e0\u30fb\u907a\u4f1d\u5b50\u914d\u5217\u60c5\u5831\u304c\u96c6\u7a4d\u3055\u308c\u305f\u3053\u3068\u306b\u3088\u308b\u3082\u306e\u3067\u3059\u3002\u4eca\u5f8c\u3082\u3001\u7d30\u80de\u6750\u6599\u306e\u54c1\u8cea\u7ba1\u7406\u65b9\u6cd5\u306b\u3001\u3053\u3046\u3057\u305f\u6700\u65b0\u306e\u6280\u8853\u3068\u60c5\u5831\u3092\u7a4d\u6975\u7684\u306b\u5c0e\u5165\u3057\u3001\u3088\u308a\u9ad8\u54c1\u8cea\u306a\u7d30\u80de\u6750\u6599\u306e\u63d0\u4f9b\u306b\u52aa\u3081\u3066\u3044\u304f\u6240\u5b58\u3067\u3059\u3002
\n\u3000\u5f15\u304d\u7d9a\u304d\u3001\u7406\u7814\u30d0\u30a4\u30aa\u30ea\u30bd\u30fc\u30b9\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u306e\u4e8b\u696d\u306b\u5bfe\u3057\u3066\u3054\u7406\u89e3\u3068\u3054\u652f\u63f4\u3092\u8cdc\u308a\u305f\u304f\u3001\u5b9c\u3057\u304f\u304a\u9858\u3044\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<\/p>\n

\u3010\u6587\u732e\u3011<\/p>\n

    \n
  1. Ono, K. et al. Species identification of animal cells by nested PCR targeted to mitochondrial DNA. In Vitro Cell Dev Biol Anim 43: 168-175 (2007)<\/li>\n
  2. Almeida, J.L., Cole, K.D., and Plant, A.L. Standards for Cell Line Authentication and Beyond. PLoS Biol 14: e1002476 (2016)<\/li>\n
  3. Yoshino, K. et al. Essential role for gene profiling analysis in the authentication of human cell lines. Hum Cell 19: 43-48 (2006)<\/li>\n
  4. Huang, Z., Yang, C., and Ke, D. DNA barcoding and molecular phylogeny in Ranidae. Mitochondrial DNA A DNA Mapp Seq Anal 27: 4003-4007 (2016)<\/li>\n
  5. Yoshino, K. et al. Development of a simple method to determine the mouse strain from which cultured cell lines originated. Interdisciplinary Bio Central 2: Article No. 14 (open access journal) doi: 10.4051\/ibc.2010.2.4.0014 (2010)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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