{"id":11426,"date":"2018-09-28T11:41:45","date_gmt":"2018-09-28T02:41:45","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/ja\/?page_id=11426"},"modified":"2025-05-21T16:20:59","modified_gmt":"2025-05-21T07:20:59","slug":"dna_barcoding","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/ja\/quality\/dna_barcoding","title":{"rendered":"DNA\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u691c\u67fb"},"content":{"rendered":"

\u5f53\u5ba4\u3067\u306f\u3001\u7d30\u80de\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u691c\u67fb\u3068\u3057\u3066\u3001DNA\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u3059\u3002
\n\u3053\u306e\u691c\u67fb\u306fDNA \u30d0\u30fc\u30b3\u30fc\u30c9\uff08\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u9818\u57df\u306e\u77ed\u3044\u5869\u57fa\u914d\u5217\uff09*1<\/sup> \u3092PCR\u5897\u5e45\u3057\u3001\u305d\u306ePCR\u7523\u7269\u306eDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u884c\u3044\u307e\u3059\u3002\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7d50\u679c\u304c\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9*2<\/sup> \u306b\u767b\u9332\u3055\u308c\u3066\u3044\u308b\u5404\u7a2e\u52d5\u7269\u306e\u5869\u57fa\u914d\u5217\u306e\u3069\u308c\u3068\u4e00\u81f4\u3059\u308b\u304b\u3092\u8abf\u3079\u308b\u3053\u3068\u3067\u3001\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u6280\u8853\u306e\u9032\u6b69\u306b\u4f34\u3063\u3066\u3001\u69d8\u3005\u306a\u52d5\u7269\u7a2e\u3067DNA \u30d0\u30fc\u30b3\u30fc\u30c9\u306e\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u304c\u5b9f\u65bd\u3055\u308c\u3001\u305d\u306e\u89e3\u6790\u7d50\u679c\u304c\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306b\u767b\u9332\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u305f\u3081\u306b\u52d5\u7269\u7a2e\u540c\u5b9a\u3078\u306e\u5fdc\u7528\u304c\u53ef\u80fd\u3068\u306a\u3063\u305f\u691c\u67fb\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n

*1<\/sup> \u5f53\u5ba4\u7d30\u80de\u682a\u306e\u7531\u6765\u52d5\u7269\u7a2e\u540c\u5b9a\u3067\u89e3\u6790\u306b\u7528\u3044\u3066\u3044\u308bDNA \u30d0\u30fc\u30b3\u30fc\u30c9
\n\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306eCytochrome c oxidase subunit I (COI) \u907a\u4f1d\u5b50\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002
\nCOI\u907a\u4f1d\u5b50\u306e\u307f\u306e\u89e3\u6790\u3067\u52d5\u7269\u7a2e\u306e\u540c\u5b9a\u306b\u81f3\u3089\u306a\u3044\u5834\u5408\u3001\u305d\u306e\u4ed6\u306e\u907a\u4f1d\u5b50\u914d\u5217\u60c5\u5831\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u308b\u52d5\u7269\u7a2e\u3067\u3042\u308c\u3070\u3001Cytochrome b (Cytb) , 16S rRNA\u306a\u3069\u305d\u306e\u4ed6\u306e\u907a\u4f1d\u5b50\u3082\u89e3\u6790\u306e\u5bfe\u8c61\u3068\u3057\u307e\u3059\u3002<\/p>\n

*2<\/sup> \u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9
\nBarcode of Life Data Systems\uff08BOLD\uff09\u3000
\nhttp:\/\/www.boldsystems.org\/<\/a><\/p>\n

National Center for Biotechnology Information – BLAST (Basic Local Alignment Search Tool)
\nhttps:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi<\/a><\/p>\n

1. \u691c\u67fb\u306e\u6d41\u308c<\/strong>
\n\u2460 DNA\u62bd\u51fa\u3000\u2192 \u2461 DNA\u30d0\u30fc\u30b3\u30fc\u30c9\u9818\u57df\u306ePCR\u5897\u5e45\u3000\u2192 \u2462 \u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308b\u78ba\u8a8d\u2192 \u2463 \u5897\u5e45\u7523\u7269\u306e\u7cbe\u88fd\u3000\u2192 \u2465 DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3000\u2192 \u2466 \u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306b\u3088\u308b\u89e3\u6790<\/p>\n

2. \u6e96\u5099\u3059\u308b\u3082\u306e<\/strong>
\n(1) \u691c\u4f53\uff1a\u7d30\u80de\u304b\u3089\u62bd\u51fa\u3057\u305f\u30b2\u30ce\u30e0DNA\u6eb6\u6db2
\n(2) \u6a5f\u5668\uff1a\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u3000Applied Biosystems®<\/sup> Veriti®<\/sup> Thermal Cycler \u7b49
\n(3) PCR\u30d7\u30e9\u30a4\u30de\u30fc
\nA\uff09COI\u907a\u4f1d\u5b50\u3092\u5897\u5e45\u3059\u308b\u30ab\u30af\u30c6\u30eb\u30d7\u30e9\u30a4\u30de\u30fc\u3000Universal primer cocktails\u3000\u203b\u5e45\u5e83\u3044\u52d5\u7269\u7a2e\u306b\u5bfe\u3057\u3066\u5897\u5e45\u304c\u5f97\u3089\u308c\u307e\u3059
\nMammalian primer mix for routine COI barcoding (Ivanova et al, 2007. Mol Ecol Notes 7:544-548)
\n\u203b\u539f\u6cd5\u3067\u306f\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7528\u306bM13\u914d\u5217\u304c\u4ed8\u4e0e\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u5f53\u5ba4\u3067\u306fForward\u306bSP6\u3001Reverse\u306bT7\u914d\u5217\u3092\u4ed8\u4e0e\u3057\u3066\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

C_VF1LFt1_SP6\u3000Forward primer cocktails (1:1:1:3\u306e\u6bd4\u7387\u3067\u6df7\u5408\u3059\u308b)<\/p>\n

\n
\n
1x LepF1_t1\uff1a\n<\/div>\n
5\u2019- ATTTAGGTGACACTATAG<\/span>A<\/span>T<\/span>T<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>A<\/span>T<\/span>C<\/span>A<\/span>T<\/span>A<\/span>A<\/span>A<\/span>G<\/span>A<\/span>T<\/span>A<\/span>T<\/span>T<\/span>G<\/span>G<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
1x VF1_t1\uff1a\n<\/div>\n
5\u2019- ATTTAGGTGACACTATAG<\/span>T<\/span>C<\/span>T<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>C<\/span>A<\/span>A<\/span>A<\/span>G<\/span>A<\/span>C<\/span>A<\/span>T<\/span>T<\/span>G<\/span>G<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
1x VF1d_t1\uff1a\n<\/div>\n
5\u2019- ATTTAGGTGACACTATAG<\/span>T<\/span>C<\/span>T<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>C<\/span>A<\/span>A<\/span>RG<\/span>A<\/span>YA<\/span>T<\/span>YG<\/span>G<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
3x VF1i_t1\uff1a\n<\/div>\n
5\u2019- ATTTAGGTGACACTATAG<\/span>T<\/span>C<\/span>T<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>A<\/span>C<\/span>C<\/span>A<\/span>NA<\/span>A<\/span>NG<\/span>A<\/span>NA<\/span>T<\/span>NG<\/span>G<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n<\/div>\n

C_VR1LRt1_T7\u3000Reverse primer cocktails (1:1:1:3\u306e\u6bd4\u7387\u3067\u6df7\u5408\u3059\u308b)<\/p>\n

\n
\n
1x LepR1_t1\uff1a\n<\/div>\n
5\u2019- TAATACGACTCACTATAGGG<\/span>T<\/span>A<\/span>A<\/span>A<\/span>C<\/span>T<\/span>T<\/span>C<\/span>T<\/span>G<\/span>G<\/span>A<\/span>T<\/span>G<\/span>T<\/span>C<\/span>C<\/span>A<\/span>A<\/span>A<\/span>A<\/span>A<\/span>A<\/span>T<\/span>C<\/span>A<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
1x VR1_t1\uff1a\n<\/div>\n
5\u2019- TAATACGACTCACTATAGGG<\/span>T<\/span>A<\/span>G<\/span>A<\/span>C<\/span>T<\/span>T<\/span>C<\/span>T<\/span>G<\/span>G<\/span>G<\/span>T<\/span>G<\/span>G<\/span>C<\/span>C<\/span>A<\/span>A<\/span>A<\/span>G<\/span>A<\/span>A<\/span>T<\/span>C<\/span>A<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
1x VR1d_t1\uff1a\n<\/div>\n
5\u2019- TAATACGACTCACTATAGGG<\/span>T<\/span>A<\/span>G<\/span>A<\/span>C<\/span>T<\/span>T<\/span>C<\/span>T<\/span>G<\/span>G<\/span>G<\/span>T<\/span>G<\/span>G<\/span>C<\/span>C<\/span>RA<\/span>A<\/span>RA<\/span>A<\/span>YC<\/span>A<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
3x VR1i_t1\uff1a\n<\/div>\n
5\u2019- TAATACGACTCACTATAGGG<\/span>T<\/span>A<\/span>G<\/span>A<\/span>C<\/span>T<\/span>T<\/span>C<\/span>T<\/span>G<\/span>G<\/span>G<\/span>T<\/span>G<\/span>NC<\/span>C<\/span>NA<\/span>A<\/span>NA<\/span>A<\/span>NC<\/span>A<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n<\/div>\n

B\uff09COI\u907a\u4f1d\u5b50\u3092\u5897\u5e45\u3059\u308b\u52d5\u7269\u7a2e\u7279\u7570\u7684\u30d7\u30e9\u30a4\u30de\u30fc\u3000\u203b\u7279\u5b9a\u306e\u52d5\u7269\u7a2e\u306b\u5bfe\u3057\u3066\u5897\u5e45\u304c\u5f97\u3089\u308c\u307e\u3059
\n\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u306fA) \u306e\u30ab\u30af\u30c6\u30eb\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u3046\u307e\u304f\u5897\u5e45\u3067\u304d\u306a\u3044 \u3042\u308b\u3044\u306f \u3046\u307e\u304f\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u304c\u8aad\u3081\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002
\n\u3082\u3057\u691c\u67fb\u5bfe\u8c61\u306e\u52d5\u7269\u7a2e\u306b\u7279\u7570\u7684\u306aCOI\u907a\u4f1d\u5b50\u30d7\u30e9\u30a4\u30de\u30fc \u3042\u308b\u3044\u306f COI\u907a\u4f1d\u5b50\u306e\u5869\u57fa\u914d\u5217\u60c5\u5831\u304c\u516c\u958b\u3055\u308c\u3066\u3044\u308b\u306e\u3067\u3042\u308c\u3070\u3001\u7279\u7570\u7684\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f5c\u88fd\u3057\u3066\u5897\u5e45\u3057\u307e\u3059\u3002<\/p>\n

C) COI\u907a\u4f1d\u5b50\u4ee5\u5916\u306eDNA\u30d0\u30fc\u30b3\u30fc\u30c9\u5897\u5e45\u7528\u30d7\u30e9\u30a4\u30de\u30fc
\nCOI\u907a\u4f1d\u5b50\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u767b\u9332\u72b6\u6cc1\u306f\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u69d8\u3005\u3067\u3059\u3002\u7279\u306b \u201c\u30a2\u30b8\u30a2\u56fa\u6709\u7a2e\u201d \u3084 \u540c\u3058\u52d5\u7269\u7a2e\u3067\u3042\u3063\u3066\u3082\u751f\u606f\u57df\u306e\u7570\u306a\u308b \u201c\u4e9c\u7a2e\u201d \u3092\u540c\u5b9a\u3057\u305f\u3044\u5834\u5408\u3001\u307e\u3063\u305f\u304f\u767b\u9332\u304c\u306a\u3044 \u3042\u308b\u3044\u306f \u5c11\u306a\u3044\u70ba\u306b\u3001\u540c\u5b9a\u306b\u81f3\u3089\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002
\n\u3053\u306e\u69d8\u306a\u30b1\u30fc\u30b9\u3067\u306f\u3001\u5bfe\u8c61\u52d5\u7269\u306e\u7a2e\u30fb\u4e9c\u7a2e\u306b\u7279\u7570\u7684\u306a\u305d\u306e\u4ed6\u306e\u907a\u4f1d\u5b50\u3092\u7528\u3044\u305f\u89e3\u6790\u65b9\u6cd5\u304c\u306a\u3044\u304b\u533b\u5b66\u6587\u732e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u7b49\u3067\u691c\u7d22\u3057\u3001\u305d\u308c\u3089\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u4f5c\u88fd\u3057\u3066\u5897\u5e45\u3057\u307e\u3059\u3002<\/p>\n

D) \u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc
\nA) ~ C) \u306e\u907a\u4f1d\u5b50\u5897\u5e45\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3001SP6\u30fbT7\u306e\u69d8\u306a\u6c4e\u7528\u914d\u5217\u3092\u4ed8\u4e0e\u3057\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u6c4e\u7528\u914d\u5217\u3092\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u3057\u3066\u7528\u3044\u307e\u3059\u3002\u6c4e\u7528\u914d\u5217\u3092\u4ed8\u4e0e\u3057\u3066\u3044\u306a\u3044\u5834\u5408\u306f\u3001\u907a\u4f1d\u5b50\u5897\u5e45\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u540c\u3058\u3082\u306e\u3092\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u7528\u3044\u307e\u3059\u3002<\/p>\n

\n
\n
SP6\uff1a\n<\/div>\n
5\u2019- ATTTAGGTGACACTATAG<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n
\n
T7\uff1a\n<\/div>\n
5\u2019- TAATACGACTCACTATAGGG<\/span> -3\u2019<\/tt>\n<\/div>\n<\/div>\n<\/div>\n

3. \u65b9\u6cd5<\/strong>
\n\u203b \u3053\u3053\u3067\u7d39\u4ecb\u3059\u308b\u306e\u306f\uff62A) COI\u907a\u4f1d\u5b50\u3092\u5897\u5e45\u3059\u308b\u30ab\u30af\u30c6\u30eb\u30d7\u30e9\u30a4\u30de\u30fc\uff63\u3092\u7528\u3044\u308b\u5834\u5408\u3067\u3059\u3002
\n\u305d\u306e\u4ed6\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u7528\u3044\u305fPCR\u6761\u4ef6\u306b\u3064\u3044\u3066\u306f\u6587\u732e \u7b49\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n

(1) PCR Reaction Mixture\uff081\u53cd\u5fdc\u5206\uff09
\n\u203b\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u524d\u306b\u30d0\u30f3\u30c9\u306e\u5207\u308a\u51fa\u3057\u7cbe\u88fd\u3092\u5fc5\u8981\u3068\u3059\u308b\u5834\u5408\u306f\u6570\u53cd\u5fdc\u5206\u3092\u7528\u610f\u3057\u307e\u3059\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
10X Ex Taq Buffer<\/td>\n5.0 \u03bcL<\/td>\n<\/tr>\n
dNTP Mixture (2.5 mM each)\t<\/td>\n4.0 \u03bcL<\/td>\n<\/tr>\n
Forward Primer [C_VF1LFt1_SP6] (10 \u03bcM)<\/td>\n0.5 \u03bcL<\/td>\n<\/tr>\n
Reverse Primer [C_VR1LRt1_T7] (10 \u03bcM)<\/td>\n0.5 \u03bcL<\/td>\n<\/tr>\n
TaKaRa Ex Taq HS (5 units\/\u03bcL)<\/td>\n0.25 \u03bcL<\/td>\n<\/tr>\n
DDW<\/td>\n37.75 \u03bcL<\/td>\n<\/tr>\n
\n
\n<\/td>\n<\/tr>\n
Total<\/td>\n48.0 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

(2) PCR\u30c1\u30e5\u30fc\u30d6\u306bPCR Reaction Mixture 48 \u03bcL\u3092\u5206\u6ce8\u3057\u3001\u305d\u308c\u305e\u308c\u306b\u691c\u4f53\u306e Template DNA 20ng (10ng\/\u03bcL, 2\u03bcL) \u3092\u52a0\u3048\u308b\u3002
\n(3) PCR\u30b5\u30a4\u30af\u30eb<\/p>\n

\"pcr_cycle\"<\/a><\/p>\n

(4) \u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308b\u78ba\u8a8d\u3000\u7d50\u679c\u4f8b\uff082\uff05 \u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u4f7f\u7528\uff09<\/p>\n

\"agarose\"<\/a><\/p>\n

(5) \u5897\u5e45\u7523\u7269\u306e\u7cbe\u88fd\u30fb\u5438\u5149\u5ea6\u6e2c\u5b9a
\n\u5e02\u8ca9\u306ePCR\u7cbe\u88fd\u30ad\u30c3\u30c8\u3001\u30b2\u30eb\u5207\u308a\u51fa\u3057\u7cbe\u88fd\u30ad\u30c3\u30c8\u7b49\u3092\u7528\u3044\u3066\u5897\u5e45\u7523\u7269\u3092\u7cbe\u88fd\u3057\u307e\u3059\u3002
\n\u307e\u305f\u3001\u5897\u5e45\u7523\u7269\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u306b\u5fc5\u8981\u306a\u6fc3\u5ea6\u306b\u8abf\u6574\u3057\u307e\u3059\u3002<\/p>\n

QIAquick PCR Purification Kit (50 \/ 250)\uff08Cat No. 28104 \/ 28106\uff09
\nQIAquick Gel Extraction Kit (50 \/ 250)\uff08Cat No. 28704 \/ 28706\uff09\u7b49<\/p>\n

(6) \u30b5\u30f3\u30ac\u30fc\u6cd5\u3092\u7528\u3044\u305f\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u306b\u3088\u308bDNA\u914d\u5217\u89e3\u6790\u3092\u884c\u3044\u307e\u3059\u3002
\n\u914d\u5217\u30c7\u30fc\u30bf\u3068\u6ce2\u5f62\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u305f\u3089\u3001\u201c\u8aad\u307f\u59cb\u3081\u201d \u3068 \u201c\u8aad\u307f\u7d42\u308f\u308a\u201d \u306e\u8aad\u307f\u53d6\u308a\u4e0d\u826f\u90e8\u5206\u3092\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u3066\u30b3\u30f3\u30bb\u30f3\u30b5\u30b9\u914d\u5217\u3092\u4f5c\u6210\u3057\u307e\u3059\u3002<\/p>\n

\"sanger\"<\/a><\/p>\n

(7) \u7d50\u679c\u89e3\u6790<\/p>\n

\u300cBarcode of Life Data Systems\uff08BOLD\uff09\u300d\u306b\u4f5c\u6210\u3057\u305f\u30b3\u30f3\u30bb\u30f3\u30b5\u30b9\u914d\u5217\u3092\u5165\u529b\u3057\u3066\u89e3\u6790\u3057\u307e\u3059\u3002
\n\u89e3\u6790\u7d50\u679c\u306e\u4f8b\uff1aRCB2943 OLHdrR-e3\u306e\u5834\u5408
\nhttps:\/\/cellbank.brc.riken.jp\/cell_bank\/CellInfo\/?cellNo=RCB2943&lang=Ja<\/a><\/p>\n

\u540c\u5b9a\u3055\u308c\u305f\u52d5\u7269\u7a2e\uff1aOryzias latipes<\/em>\uff08\u30df\u30ca\u30df\u30e1\u30c0\u30ab\uff09100%<\/p>\n

\"BOLD_v5_01\"<\/a><\/p>\n

\"BOLD_v5_02\"<\/a><\/p>\n

\u30e1\u30c0\u30ab\u7531\u6765\u7d30\u80de\u682a\u306e\u89e3\u6790\u7d50\u679c Oryzias latipes<\/em><\/p>\n\n\n\n\n\n\n\n\n
\u2460 \u30e1\u30c0\u30ab(Hd-rR\u7cfb\u7d71) COI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9
https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/AB498065<\/a><\/td>\n		\u2466 \u30e1\u30c0\u30ab COI \u30b7\u30fc\u30af\u30a8\u30f3\u30b9
https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/NC_004387<\/a><\/td>\n<\/tr>\n
\u2461 RCB0187 OLHE-131 Lot.1 \uff1aOLHE-131<\/td>\n\u2467 RCB0184 OLF-136 Lot.1 \uff1aOLF-136<\/td>\n<\/tr>\n
\u2462 RCB2943 OLHdrR-e3 Lot.1 \uff1aOLHdrR-e3<\/td>\n\u2468 RCB0188 OLME-104 Lot.2 \uff1aOLME-104<\/td>\n<\/tr>\n
\u2463 RCB2944 OLCAB-e31 Lot.1 \uff1aOLCAB-e31<\/td>\n\u2469 \u30e1\u30c0\u30ab(HNI\u7cfb\u7d71) COI\u30b7\u30fc\u30af\u30a8\u30f3\u30b9
https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/AB498066<\/a><\/td>\n<\/tr>\n
\u2464 RCB2945 DIT29 Lot.1 \uff1aDIT29<\/td>\n\u246a RCB2942 OLHNI-2 Lot.1 \uff1aOLHNI-2<\/td>\n<\/tr>\n
\u2465 RCB2946 OLCAB-e3 Lot.1 \uff1aOLCAB-e3<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"medaka_3.png\"<\/a><\/p>\n

4. \u5224\u5b9a<\/strong>
\nBOLD\u300cSpecies Level Barcode Records\u300d\u3067\u306e\u89e3\u6790\u306e\u7d50\u679c\u300195\uff05\u4ee5\u4e0a\u306e\u4e00\u81f4\u7387\u3068\u306a\u308a\u3001\u5bc4\u8a17\u60c5\u5831\u3068\u52d5\u7269\u7a2e\u304c\u4e00\u81f4\u3057\u305f\u5834\u5408\u306f\u5408\u683c\u3068\u306a\u308a\u307e\u3059\u3002
\n\u300c\u4e00\u81f4\u7387\u304c\u4f4e\u3044\u300d\u300c\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u767b\u9332\u60c5\u5831\u304c\u306a\u3044 or \u5c11\u306a\u3044\u300d\u300c\u4e00\u81f4\u7387\u306f\u9ad8\u3044\u304c\u4e9c\u7a2e\u306e\u7279\u5b9a\u307e\u3067\u81f3\u3089\u306a\u3044\u300d
\n\u300c\u65b0\u305f\u306a\u77e5\u898b\u306b\u3088\u308a\u5b66\u540d\u304c\u5909\u66f4\u307e\u305f\u306f\u518d\u5206\u985e\u3055\u308c\u3066\u3044\u308b\u300d\u300c\u5b66\u540d\u306b\u5bfe\u7acb\u610f\u898b\u304c\u5b58\u5728\u3059\u308b\u300d\u306a\u3069\u3001\u52d5\u7269\u7a2e\u306b\u3088\u3063\u3066\u69d8\u3005\u306a\u30b1\u30fc\u30b9\u304c\u3042\u308a\u307e\u3059\u3002
\nCOI\u907a\u4f1d\u5b50\u3060\u3051\u3067\u306f\u540c\u5b9a\u3067\u304d\u306a\u3044\u5834\u5408\u306b\u306f\u305d\u306e\u4ed6\u306e\u907a\u4f1d\u5b50\u3092\u7528\u3044\u305f\u89e3\u6790\u65b9\u6cd5\u3082\u691c\u8a0e\u3057\u3001\u7dcf\u5408\u7684\u306a\u5224\u5b9a\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n

5. \u53c2\u8003\u6587\u732e<\/strong>
\nAlmeida, J.L., Cole, K.D., and Plant, A.L.
\nStandards for Cell Line Authentication and Beyond.
\nPLoS Biol<\/em> 14: e1002476 (2016).<\/a><\/p>\n

Hebert PD, Cywinska A, Ball SL, deWaard JR.
\nBiological identifications through DNA barcodes.
\nProc Biol Sci.<\/em> 2003 Feb 7;270(1512):313-21. doi:10.1098\/rspb.2002.2218.<\/a> PMID: 12614582<\/a>; PMCID: PMC1691236.<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5f53\u5ba4\u3067\u306f\u3001\u7d30\u80de\u306e\u7531\u6765\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u691c\u67fb\u3068\u3057\u3066\u3001DNA\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u691c\u67fb\u3092\u5b9f\u65bd\u3057\u3066\u304a\u308a\u307e\u3059\u3002 \u3053\u306e\u691c\u67fb\u306fDNA \u30d0\u30fc\u30b3\u30fc\u30c9\uff08\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u9818\u57df\u306e\u77ed\u3044\u5869\u57fa\u914d\u5217\uff09*1 \u3092PCR\u5897\u5e45\u3057\u3001\u305d\u306ePCR\u7523\u7269\u306eDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u884c\u3044\u307e\u3059\u3002\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u7d50\u679c\u304c\u516c\u958b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9*2 \u306b\u767b\u9332\u3055\u308c\u3066\u3044\u308b\u5404\u7a2e\u52d5\u7269\u306e\u5869\u57fa\u914d\u5217\u306e\u3069\u308c\u3068\u4e00\u81f4\u3059\u308b\u304b\u3092\u8abf\u3079\u308b\u3053\u3068\u3067\u3001\u52d5\u7269\u7a2e\u3092\u540c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u6280\u8853\u306e\u9032\u6b69\u306b […]<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":3425,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_mc_calendar":[],"footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-11426","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/11426","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=11426"}],"version-history":[{"count":8,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/11426\/revisions"}],"predecessor-version":[{"id":15002,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/11426\/revisions\/15002"}],"up":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/3425"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=11426"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}