{"id":9319,"date":"2017-02-09T13:24:10","date_gmt":"2017-02-09T04:24:10","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/en\/?page_id=9319"},"modified":"2024-03-11T16:45:48","modified_gmt":"2024-03-11T07:45:48","slug":"quality","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/en\/quality","title":{"rendered":"Quality Control"},"content":{"rendered":"

Our cell lines are cryopreserved under the culturing condition designated by the depositor of each cell line. We routinely test each lot of cells using the ampoules\/tubes randomly selected from a lot by thawing and culturing them for certain periods, and cryopreserve cells after confirmation that there are no problems about freeze-thaw and microbial contamination (bacteria and fungi).<\/p>\n

General Inspection<\/strong><\/p>\n\n\n\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Inspection<\/td>Human<\/td>Mouse<\/td>Other
\nmammals<\/td>		Other
\nanimals<\/td>		Hybridoma*1<\/sup><\/td>\n<\/tr>\n
Cell viability and Adhesion efficiency<\/a><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\n<\/tr>\n
Mycoplasma infection
\n(DNA staining method, PCR method)<\/a>*2<\/sup><\/td>		\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\n<\/tr>\n
Morphology<\/a><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\n<\/tr>\n
Test for misidentification
\n(Short Tandem Repeat polymorphism analysis)<\/td>		\u25cb<\/span><\/td><\/td><\/td><\/td><\/td>\n<\/tr>\n
Identification of animal species
\n(PCR method, Isozyme analysis*3<\/sup>)<\/td>		\u25cb<\/span>*4<\/sup><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td><\/td>\n<\/tr>\n
DNA barcoding analysis<\/a><\/td><\/td><\/td>\u25cb<\/span><\/td>\u25cb<\/span><\/td><\/td>\n<\/tr>\n
Identification of mouse strain
\n(SSLP analysis)<\/td>		<\/td>\u25cb<\/span>*5<\/sup><\/td><\/td><\/td><\/td>\n<\/tr>\n
Production of antibody<\/a><\/td><\/td><\/td><\/td><\/td>\u25cb<\/span><\/td>\n<\/tr>\n
Virus<\/td>HBV<\/td>Cells derived
\nfrom liver only<\/td>		<\/td><\/td><\/td><\/td>\n<\/tr>\n
HCV<\/td>Cells derived
\nfrom liver only<\/td>		<\/td><\/td><\/td><\/td>\n<\/tr>\n
HIV<\/td>Cells derived
\nfrom blood only<\/td>		<\/td><\/td><\/td><\/td>\n<\/tr>\n
HTLV-1<\/td>Cells derived
\nfrom blood only<\/td>		<\/td><\/td><\/td><\/td>\n<\/tr>\n
EBV<\/a><\/td>Cells derived from blood
\nand cancer cell lines*6<\/sup><\/td>		<\/td><\/td><\/td><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n*1<\/sup> When the hybridoma cell line is a hybrid including human blood cell, general tests for cell lines derived from human blood cells are performed.
\n*2<\/sup> Tests for mycoplasma infection are performed by “DNA-staining method” and “PCR method for genus Mycoplasma<\/em> and Acholeplasma<\/em>“.
\n*3<\/sup> Isozyme analysis is performed for some specific animal species other than the following species: human, mouse, rat, chicken, Syrian hamster, Chinese hamster, African green monkey, cynomolgus monkey, rabbit, dog, pig, cow, cat.
\n*4<\/sup> Not tested for human iPS cells (HPS), EBV-transformed B cell lines derived from Japanese (HEV) and Sonoda-Tajima Collection (HSC).
\n*5<\/sup> Not tested for mouse ES\/iPS cells which are cultured with feeder cells.
\n*6<\/sup> Tested for human cell lines derived from blood cells (but except for EBV-transformed B cell lines). This is also tested for a part of human cancer cell lines such as oral, esophageal and stomach cancer, since it has been reported that EBV-infection is a cause of these cancers in some cases.<\/p>\n

– In relation to human mesenchymal stem cells, tests for HBV, HCV, HIV and HTLV-1 are performed for a part of them but not for all. The results of tests are disclosed in the cell search column in the cases that they are performed. Providing cells are those which showed negative results for all tests.<\/p>\n

Specific Examination<\/strong>
\n\n\n\n\n\t\n\t\n\t\n\t\n\t\n\t
Examination<\/div><\/td>
Target cell etc.<\/div><\/td>
Cell lines<\/div><\/td>\n<\/tr>\n
Induction of differentiation to
\nsome specific cell type etc.<\/div><\/td>
Nerve cell<\/td>RCB0009 PC-12<\/a>
\nRCB2863 N14.5<\/a><\/td>\n<\/tr>\n
Muscle cell<\/td>RCB0987 C2C12<\/a>
\nRCB2366 Hu5\/E18<\/a><\/td>\n<\/tr>\n
Cardiomyocyte<\/td>RCB2318 P19.CL6<\/a><\/td>\n<\/tr>\n
Alkaline phosphatase (ALP)
\nactivity<\/td>		RCB1126 MC3T3-E1<\/a>(inclueded cloning sublines)<\/td>\n<\/tr>\n
Karyotype analysis<\/div><\/td>
A part of general cell lines (RCB) and a part of ES\/iPS cell lines<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\nOther Information<\/strong><\/p>\n