{"id":8402,"date":"2015-02-05T16:17:18","date_gmt":"2015-02-05T07:17:18","guid":{"rendered":"http:\/\/cell0.cell.rbrc.jp\/en\/?page_id=8402"},"modified":"2026-02-16T15:56:33","modified_gmt":"2026-02-16T06:56:33","slug":"inspection","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/en\/inspection","title":{"rendered":"Cell line validation testing"},"content":{"rendered":"

If contaminated cell lines are used in research, the data obtained may not be reproducible or dependable. Recently Nature and Science have published articles warning the research community about contamination, and the journals are considering a policy of refusing papers that use data derived from non-validated cell lines.<\/p>\n

To give one example, the editorial policy the journal Cancer Research requires that the method of validation testing by the source cell bank is described in submitted papers if the cell lines were received within six months. If more than six months have passed since receipt of the cell line, retesting is required.<\/p>\n

To respond to these needs, the Cell Engineering Division at RIKEN BRC has begun a cell line verification testing service for material which is supplied by, or is deposited in, BRC. Details are shown below.<\/p>\n

 <\/p>\n

\u25cf Cell lines supplied by RIKEN BRC (Note: A separate fee is required to obtain cell lines from BRC)<\/p>\n

    \n
  1. \u00a0Cell line validation report
    \nWhen users require validation of cell lines for publication purposes, RIKEN BRC will issue a report (i.e.,\u00a0Validation Report (1)<\/a>\u00a0,\u00a0Validation Report (3)<\/a>). The report will give the date of supply and demonstrate that cell validation testing was conducted prior to shipment. This service is free of charge.\u00a0\u00a0\u00a0How to apply: Send an email to\u00a0cellbank.brc\"\"riken.jp<\/a>\u00a0with a description of the cell line for which you require a validation report. Write “Validation testing service” in the subject line.<\/li>\n
  2. \u00a0Validation re-testing at RIKEN BRC (Only for cell lines supplied by BRC<\/span>)
    \nWe will issue a report (i.e.,\u00a0    Validation Report (2)<\/a>), if the user requires revalidation testing of cell lines originally supplied to them by RIKEN BRC. The user will pay the necessary fees for the testing and drafting of the report. (See table below.)<\/li>\n<\/ol>\n

    Inspection fees will change effective Wednesday, April 1, 2026.
    \nThe new fees will apply to applications received on or after Wednesday, April 1, 2026\u00a0 (Japan Standard Time).
    \nThe current fees will apply to properly completed applications received by Wednesday March 31, 2026 (Reiwa 8).<\/p>\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n
    Inspection items<\/td>\nInspection samples<\/td>\nCurrent fees<\/td>\nNew fees<\/td>\n<\/tr>\n
    Actual expenses required
    (tax included)<\/td>\n    		Actual expenses required
    (tax included)<\/td>\n<\/tr>\n
    Mycoplasma test<\/a><\/td>\nConditional culture medium*<\/td>\n38,610 JPY<\/td>\n50,710 JPY<\/td>\n<\/tr>\n
    Short tandem repeat polymorphism
    analysis test for human cell lines<\/td>\n    		Cellular genomic DNA or cell pellet<\/td>\n37,510 JPY<\/td>\n49,170 JPY<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    *A cell line in a conditional culture medium must pass for at least three weeks in an antibiotic-free culture, and at least three days must have elapsed since passage or exchange of culture medium.<\/p>\n

    \u00a0Note:<\/strong> We cannot conduct validation testing on cell lines supplied by other cell banks since we are not able to conduct tests on cell lines supplied by other cell banks.<\/p>\n

    How to apply:<\/p>\n