{"id":8354,"date":"2015-02-04T15:20:55","date_gmt":"2015-02-04T06:20:55","guid":{"rendered":"http:\/\/cell0.cell.rbrc.jp\/en\/?page_id=8354"},"modified":"2026-08-28T09:01:31","modified_gmt":"2026-08-28T00:01:31","slug":"medium-list","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/en\/medium-list","title":{"rendered":"Medium List"},"content":{"rendered":"<p>During the initial culture period, please begin the culture <u>under the conditions specified by our cell bank.<\/u> <strong>(Please check the datasheet)<\/strong><br \/>\nProducts from other manufacturers may be used, provided that they have an equivalent composition.<br \/>\nWe strongly suggest all users to culture the cells under the culture condition recommended by our cell bank until you make enough stocks. After that, culture the cells with the other protocols of your interest for your research purposes.<\/p>\n<ul>\n<li><strong>L-glutamine<\/strong><\/li>\n<p>L-glutamine is an essential component of various basal media. However, due to its instability in aqueous solution,<br \/>\nsome commercially available media do not contain L-glutamine. In such cases, please supplement the medium with L-glutamine.<\/p>\n<p>L-glutamine remains stable when frozen, either completely or partially. However, prolonged storage in a fully thawed state may compromise its stability, particularly at temperatures above 2\u20138 \u00b0C. For long-term storage, L-glutamine should be aliquoted and stored at \u221220 \u00b0C.<\/p>\n<li><strong>Heat inactivation of serum<\/strong><\/li>\n<p>In our cell bank, serum is not heat-inactivated unless spesifically requested by the depositor.&#8221;\t<\/p>\n<li><strong>Cttokines<\/strong><\/li>\n<p>The &#8220;r&#8221; shown in the medium compisition on the datesheet stands for recombinant.<br \/>\n\u3000\u3000ex\uff1aRPMI1640+10%FBS+10 ng\/mL mouse rIL-3&#8243;\n<\/ul>\n<h5>Basal Cell Culture Medium<\/h5>\n\n<table id=\"tablepress-129\" class=\"tablepress tablepress-id-129\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\"><strong>Cell culture media<\/strong><\/th><th class=\"column-2\"><strong>Manufacturer, Catalog Number etc<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">BME<\/td><td class=\"column-2\">Basal Medium, Eagle\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(21010046) <span style=\"color:#00B050;\"><strong>without L-Glutamine<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">CultiCell<\/td><td class=\"column-2\">Equivalent product to StemMedium<br \/>\n<a href=\"http:\/\/www.kacnet.co.jp\/\">StemMedium Serum Free Media for Mouse ES Cell\u00a0\u00a0<strong>KAC co<\/strong>\u00a0 (KSDSRK100)<u>Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">Defined Keratinocyte-SFM<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(10744019)<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">DM-160AU<\/td><td class=\"column-2\"><a href=\"https:\/\/www.kyokutoseiyaku.co.jp\/\">(Ref.: Katsuta, H. and Takaoka, T. : Methods in Cell Biol. 14, 145-158,1976)\u00a0\u00a0<strong>KYOKUTO PHARMACEUTICAL INDUSTRIAL CO., LTD<\/strong>\u00a0<u>\u00a0Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">DM-201<\/td><td class=\"column-2\"><a href=\"https:\/\/www.kyokutoseiyaku.co.jp\/\"><strong>KYOKUTO PHARMACEUTICAL INDUSTRIAL CO., LTD<\/strong><u>\u00a0\u00a0Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">DMEM (low glucose)<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11885084)<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\">DMEM (high glucose)<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11995065)<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\">DMEM (high glucose, without Sodium pyruvate)<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11965092) or <strong>SIGMA<\/strong>\u00a0\u00a0(D5796)<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\">DMEM (high glucose, without L-Glutamine, with Sodium pyruvate)<\/td><td class=\"column-2\"><strong>GIBCO <\/strong>(10313021) <span style=\"color:#00B050;\"><strong>without L-Glutamine<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\">DMEM (high glucose, HEPES) <\/td><td class=\"column-2\"><strong>GIBCO<\/strong> (12430054)<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\">DMEM (KnockOut)<\/td><td class=\"column-2\">KnockOut DMEM\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(10829018) <span style=\"color:#00B050;\"><strong>without L-Glutamine<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\">DMEM\/HamF12<\/td><td class=\"column-2\">DMEM\/HamF12 (with L-Glutamine)\u00a0<strong>GIBCO <\/strong>(11320033, L-Glu 2.5mM, without HEPES) or\u00a0<strong>FUJIFILM Wako Pure Chemical Corporation <\/strong>(048-29785, L-Glu 2.5mM, without HEPES)<\/td>\n<\/tr>\n<tr class=\"row-14\">\n\t<td class=\"column-1\">DMEM\/HamF12 (without L-Glutamine)<\/td><td class=\"column-2\">DMEM\/HamF12 (<span style=\"color:#00B050;\"><strong>without L-Glutamine<\/strong><\/span>, with HEPES)\u00a0<strong>SIGMA<\/strong>\u00a0\u00a0\u00a0(D6421)<\/td>\n<\/tr>\n<tr class=\"row-15\">\n\t<td class=\"column-1\">DMEM\/HamF12 (with HEPES, with Sodium pyruvate)<\/td><td class=\"column-2\">DMEM\/HamF12 (with L-Glutamine 2.5mM, HEPES, Sodium pyruvate) <strong>SIGMA <\/strong>(D8437)<\/td>\n<\/tr>\n<tr class=\"row-16\">\n\t<td class=\"column-1\">DMEM\/HamF12 (with GlutaMax, without HEPES)<\/td><td class=\"column-2\">DMEM\/HamF12, GlutaMAX supplement 2.5mM, <strong>GIBCO<\/strong> (10565018)<\/td>\n<\/tr>\n<tr class=\"row-17\">\n\t<td class=\"column-1\">Earle's BSS<\/td><td class=\"column-2\">Earle's Balanced Salt Solution (EBSS) <strong> GIBCO<\/strong> (14155063)<\/td>\n<\/tr>\n<tr class=\"row-18\">\n\t<td class=\"column-1\">E-RDF<\/td><td class=\"column-2\"><strong>KYOKUTO PHARMACEUTICAL INDUSTRIAL CO., LTD\u00a0\u00a0<\/strong>\u00a0(26500)<\/td>\n<\/tr>\n<tr class=\"row-19\">\n\t<td class=\"column-1\">ES<\/td><td class=\"column-2\">ES medium \"Nissui\"\u00a0\u00a0has been discontinued. <br \/>\nThe composition of this medium is \"Eagle MEM(with L-glutamine), Non-Essential Amino Acids 0.2mM, sodium pyruvate 1mM, vitamine B12 0.1 mg\/L\".<\/td>\n<\/tr>\n<tr class=\"row-20\">\n\t<td class=\"column-1\">EX-CELL CD CHO Fusion<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0(14365C)<\/td>\n<\/tr>\n<tr class=\"row-21\">\n\t<td class=\"column-1\">G-031101<\/td><td class=\"column-2\"><a href=\"https:\/\/www.emukk.com\/WP\/\"><strong>Mx\u00a0<\/strong>(31101)\u00a0<u>Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-22\">\n\t<td class=\"column-1\">GIT<\/td><td class=\"column-2\">Manufacturer: <strong> Kohjin-bio Co.,Ltd <\/strong>(16041030)\u3001Distributor: <strong>FUJIFILM Wako<\/strong> (637-25715)<\/td>\n<\/tr>\n<tr class=\"row-23\">\n\t<td class=\"column-1\">GMEM<\/td><td class=\"column-2\">Glasgow's MEM\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(11710035)<\/td>\n<\/tr>\n<tr class=\"row-24\">\n\t<td class=\"column-1\">Grace's Insect Medium, Supplemented<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11605094)<\/td>\n<\/tr>\n<tr class=\"row-25\">\n\t<td class=\"column-1\">Grace's Insect Medium, Unsupplemented<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11595030)<\/td>\n<\/tr>\n<tr class=\"row-26\">\n\t<td class=\"column-1\">HamF10<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0\u00a0(N6908)<\/td>\n<\/tr>\n<tr class=\"row-27\">\n\t<td class=\"column-1\">HamF12<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0\u00a0(N6658)<\/td>\n<\/tr>\n<tr class=\"row-28\">\n\t<td class=\"column-1\">Hybridoma Serum-Free Medium<\/td><td class=\"column-2\"><strong>FUJIFILM Wako<\/strong> (081-10381) <\/td>\n<\/tr>\n<tr class=\"row-29\">\n\t<td class=\"column-1\">RITC80-7<\/td><td class=\"column-2\"><strong>Research Institute for the Functional Peptides Co., Ltd <\/strong>  (IFP-0150)<\/td>\n<\/tr>\n<tr class=\"row-30\">\n\t<td class=\"column-1\">HuMedia-KG2<\/td><td class=\"column-2\"><strong>KURABO <\/strong>\u00a0\u00a0(KK-2150S)<\/td>\n<\/tr>\n<tr class=\"row-31\">\n\t<td class=\"column-1\">IMDM<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0\u00a0(I3390) <span style=\"color:#00B050;\"><strong>without L-Glutamine<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-32\">\n\t<td class=\"column-1\">Iscove's<\/td><td class=\"column-2\">Iscove's Modified Dulbecco's Medium: IMDM<\/td>\n<\/tr>\n<tr class=\"row-33\">\n\t<td class=\"column-1\">IPL-41<\/td><td class=\"column-2\">IPL-41 Insect medium,\u00a0<strong>GIBCO<\/strong>\u00a0(11405081)<\/td>\n<\/tr>\n<tr class=\"row-34\">\n\t<td class=\"column-1\">iPSellon<\/td><td class=\"column-2\"><a href=\"http:\/\/www.cardio.co.jp\/\">Cardio Incorporated (007001)<u> Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-35\">\n\t<td class=\"column-1\">KBM 353<\/td><td class=\"column-2\"><strong>Kohjin-bio Co.,Ltd <\/strong>(16023530)<\/td>\n<\/tr>\n<tr class=\"row-36\">\n\t<td class=\"column-1\">Keratinocyte-SFM<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0(17005042)<\/td>\n<\/tr>\n<tr class=\"row-37\">\n\t<td class=\"column-1\">L-15<\/td><td class=\"column-2\">Leibovitz\u00a0\u00a0<strong>SIGMA<\/strong>\u00a0\u00a0(L1518)<\/td>\n<\/tr>\n<tr class=\"row-38\">\n\t<td class=\"column-1\">M199<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11150059)<span style=\"color:#00B050;\"><strong>without HEPES<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-39\">\n\t<td class=\"column-1\">M199 (20-25mM HEPES)<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(12340030)<\/td>\n<\/tr>\n<tr class=\"row-40\">\n\t<td class=\"column-1\">McCoy's 5A<\/td><td class=\"column-2\">McCoy's 5A (Modified) Medium\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(16600082)<\/td>\n<\/tr>\n<tr class=\"row-41\">\n\t<td class=\"column-1\">MSCGM<\/td><td class=\"column-2\"><strong>LONZA<\/strong> PT-3001<\/td>\n<\/tr>\n<tr class=\"row-42\">\n\t<td class=\"column-1\">MEM<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0\u00a0(M4655\uff09<\/td>\n<\/tr>\n<tr class=\"row-43\">\n\t<td class=\"column-1\">MEM ALPHA<\/td><td class=\"column-2\">MEM\u03b1, nucleosides <strong>GIBCO<\/strong>\u00a0\u00a0(12571-063)<br \/>\n<span style=\"color:#00B050;\"><strong>with Ribonucleosides, Deoxyribonucleosides<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-44\">\n\t<td class=\"column-1\">MEM(suspension)<\/td><td class=\"column-2\">S-MEM\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(11380-037) <span style=\"color:#00B050;\"><strong>without  L-Glutamine<\/strong><\/span><\/td>\n<\/tr>\n<tr class=\"row-45\">\n\t<td class=\"column-1\">MM<\/td><td class=\"column-2\">Mitsuhashi &amp; Maramorosch insect tissue culture medium (Ref.: Singh, K.R.P.,Curr. Sci. 36, 506, 1967\uff09\u3000<strong>HiMedia<\/strong> (IML002)<\/td>\n<\/tr>\n<tr class=\"row-46\">\n\t<td class=\"column-1\">NCTC109<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0(N1140)<\/td>\n<\/tr>\n<tr class=\"row-47\">\n\t<td class=\"column-1\">NCTC135<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0(N3262)<\/td>\n<\/tr>\n<tr class=\"row-48\">\n\t<td class=\"column-1\">Neurobasal Medium (NBM)<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0(21103-049)<\/td>\n<\/tr>\n<tr class=\"row-49\">\n\t<td class=\"column-1\">Pluronic F-68<\/td><td class=\"column-2\">This product has been discontinued. The replacement is Kolliphor P 118 (SIGMA, K4894)<\/td>\n<\/tr>\n<tr class=\"row-50\">\n\t<td class=\"column-1\">PLUSOID-M<\/td><td class=\"column-2\"><a href=\"https:\/\/www.emukk.com\/WP\/\"><strong>Mx\u00a0<\/strong>(31101)\u00a0<u>Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-51\">\n\t<td class=\"column-1\">POWEREDBY10<\/td><td class=\"column-2\"><a href=\"https:\/\/www.emukk.com\/WP\/\"><strong>Mx<\/strong>\u00a0(31101)<u>\u00a0Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-52\">\n\t<td class=\"column-1\">Primate ES Cell Medium<\/td><td class=\"column-2\"><strong>ReproCELL<\/strong>\u00a0\u00a0(RCHEMD001)<\/td>\n<\/tr>\n<tr class=\"row-53\">\n\t<td class=\"column-1\">RDF (Glutamine(-))<\/td><td class=\"column-2\"><strong>Research Institute for the Functional Peptides Co., Ltd<\/strong><\/td>\n<\/tr>\n<tr class=\"row-54\">\n\t<td class=\"column-1\">RPMI 1640<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(11875093)<\/td>\n<\/tr>\n<tr class=\"row-55\">\n\t<td class=\"column-1\">S-Clone CM-B<\/td><td class=\"column-2\"><strong>SEKISUI CHEMICAL CO., LTD. <\/strong>\u00a0(521669)\u00a0<\/td>\n<\/tr>\n<tr class=\"row-56\">\n\t<td class=\"column-1\">Schneider's<\/td><td class=\"column-2\">Schneider's drosophila medium\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(21720024)<\/td>\n<\/tr>\n<tr class=\"row-57\">\n\t<td class=\"column-1\">SFM-101<\/td><td class=\"column-2\">SFM101 NISSUI \u00a0<strong>Shimadzu Diagnostiics Corporation<\/strong>\u00a0\u00a0(05963)<\/td>\n<\/tr>\n<tr class=\"row-58\">\n\t<td class=\"column-1\">StemFit AK02N<\/td><td class=\"column-2\"><strong>Ajinomoto, Tokyo, Japan (AK02N)<\/strong><\/td>\n<\/tr>\n<tr class=\"row-59\">\n\t<td class=\"column-1\">StemMedium<\/td><td class=\"column-2\"><a href=\"http:\/\/www.kacnet.co.jp\/\">StemMedium Serum Free Media for Mouse ES Cell\u00a0\u00a0<strong>KAC co\u00a0<\/strong> (KSDSRK100)\u00a0<u>\u00a0Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-60\">\n\t<td class=\"column-1\">Stem Pro-34 SFM<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(10639011)<\/td>\n<\/tr>\n<tr class=\"row-61\">\n\t<td class=\"column-1\">Stem Span SFEM<\/td><td class=\"column-2\"><strong>Stemcell Technologies Inc<\/strong>\u00a0\u00a0(ST-09650)<\/td>\n<\/tr>\n<tr class=\"row-62\">\n\t<td class=\"column-1\">STK2<\/td><td class=\"column-2\"><a href=\"http:\/\/www.twocells.com\/\">Serum Free Medium for Mesenchymal Stem Cell <strong>TWOCELLS COMPANY, LIMITED<\/strong> (KBDSTC102) <u>Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-63\">\n\t<td class=\"column-1\">TC-100<\/td><td class=\"column-2\"><strong>Baculotechnologies Company Limited<\/strong> or <strong>Sigma-Aldrich<\/strong><\/td>\n<\/tr>\n<tr class=\"row-64\">\n\t<td class=\"column-1\">William's E<\/td><td class=\"column-2\">William's medium E,\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(12551032)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-129 from cache -->\n<h5>Reagents<\/h5>\n\n<table id=\"tablepress-130\" class=\"tablepress tablepress-id-130\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\"><strong>Reagents<\/strong><\/th><th class=\"column-2\"><strong>Manufacturer, Catalog Number etc<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">B27<\/td><td class=\"column-2\">B27 Supplement (50X)\u00a0<strong>GIBCO\u00a0<\/strong>(17504044)<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">Bacto-Peptone<\/td><td class=\"column-2\"><strong>BD<\/strong>\u00a0(211677)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">BES : N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0(B9879)<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">BIO : (2'Z,3'E)-6-Bromoindirubin-3'-acetoxime<\/td><td class=\"column-2\">BIO-Acetoxime (GSK-3 Inhibitor X )\u00a0<strong>Merck <\/strong>(361551)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">BM Condimed H1<\/td><td class=\"column-2\"><strong>Roche<\/strong>\u00a0(04155645001)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">bovine apo-Transferrin<\/td><td class=\"column-2\">Transferrin, Bovine (Apo form), lyophilized\u00a0<strong>GIBCO\u00a0<\/strong>(11108016)<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\">Dispase<\/td><td class=\"column-2\">Dispase I\u3000<strong>\u00a0FUJIFILM Wako Pure Chemical Corporation <\/strong>(386-02271)<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\">Dispase II<\/td><td class=\"column-2\"><strong>Roche<\/strong>\u00a0(4942078001)<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\">Earle's BSS<\/td><td class=\"column-2\">Earle's Balanced Salt Solution\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(14155063)<\/td>\n<\/tr>\n<tr class=\"row-11\">\n\t<td class=\"column-1\">G418<\/td><td class=\"column-2\">G418 disulfate salt solution (50mg\/ml)\u00a0\u00a0<strong>SIGMA<\/strong>\u00a0\u00a0(G8168)<\/td>\n<\/tr>\n<tr class=\"row-12\">\n\t<td class=\"column-1\">HAT<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(21060017)<\/td>\n<\/tr>\n<tr class=\"row-13\">\n\t<td class=\"column-1\">HEPES<\/td><td class=\"column-2\"><strong>GIBCO<\/strong>\u00a0\u00a0(15630080)<\/td>\n<\/tr>\n<tr class=\"row-14\">\n\t<td class=\"column-1\">human Insulin<\/td><td class=\"column-2\">Insulin solution human,\u00a0<strong>SIGMA\u00a0<\/strong>(I9278)<\/td>\n<\/tr>\n<tr class=\"row-15\">\n\t<td class=\"column-1\">Insulin<\/td><td class=\"column-2\">Insulin solution from Bovine Pancreas\u00a0<strong>SIGMA<\/strong>\u00a0(I0516)<\/td>\n<\/tr>\n<tr class=\"row-16\">\n\t<td class=\"column-1\">IWP-2<\/td><td class=\"column-2\"><a href=\"https:\/\/reprocell.co.jp\/\">Wnt Inhibitor IWP-2,\u00a0<strong>Stemgent<\/strong>\u00a0(04-0034)<u>\u00a0<\/u><u>Contact<\/u><\/a><\/td>\n<\/tr>\n<tr class=\"row-17\">\n\t<td class=\"column-1\">KSR<\/td><td class=\"column-2\">KnockOut Serum Replacement (KSR)\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(10828028)<\/td>\n<\/tr>\n<tr class=\"row-18\">\n\t<td class=\"column-1\">L-Glutamine<\/td><td class=\"column-2\">L-Glutamine 200mM (100X)\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(25030081)<\/td>\n<\/tr>\n<tr class=\"row-19\">\n\t<td class=\"column-1\">MEM Amino Acids Solution<\/td><td class=\"column-2\">MEM Amino Acids Solution (50x)\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(11130051)<\/td>\n<\/tr>\n<tr class=\"row-20\">\n\t<td class=\"column-1\">MEM Vitamine Solution<\/td><td class=\"column-2\">MEM Vitamine Solution (100x)\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(11120052)<\/td>\n<\/tr>\n<tr class=\"row-21\">\n\t<td class=\"column-1\">NEAA<\/td><td class=\"column-2\">Non-Essential Amino Acids 10mM (100x), liquid 100ml\u00a0\u00a0<strong>GIBCO<\/strong>\u00a0\u00a0(11140050)<\/td>\n<\/tr>\n<tr class=\"row-22\">\n\t<td class=\"column-1\">NEAA (ES Cell Qualified)<\/td><td class=\"column-2\">ES Cell Qualified MEM Non-Essential Amino Acids Solution 10mM (100X)\u00a0\u00a0<strong>\u00a0Merck<\/strong>\u00a0\u00a0(TMS-001-C)<\/td>\n<\/tr>\n<tr class=\"row-23\">\n\t<td class=\"column-1\">Nucleosides (ES Cell Qualified)<\/td><td class=\"column-2\">ES Cell Qualified Nucleosides (100X)\u00a0\u00a0<strong>\u00a0Merck<\/strong>\u00a0(ES-008-D)<\/td>\n<\/tr>\n<tr class=\"row-24\">\n\t<td class=\"column-1\">T3<\/td><td class=\"column-2\">3, 3', 5-Triiodo-L-thyronine,\u00a0<strong>SIGMA<\/strong>\u00a0(T2877)<\/td>\n<\/tr>\n<tr class=\"row-25\">\n\t<td class=\"column-1\">Transferrin<\/td><td class=\"column-2\"><strong>SIGMA<\/strong>\u00a0\u00a0(T1283\uff09<\/td>\n<\/tr>\n<tr class=\"row-26\">\n\t<td class=\"column-1\">Ultroser G<\/td><td class=\"column-2\">Ultroser G serum substitute<\/td>\n<\/tr>\n<tr class=\"row-27\">\n\t<td class=\"column-1\">Y-27632<\/td><td class=\"column-2\">CultureSure Y-27632, <strong>FUJIFILM Wako Pure Chemical Corporation<\/strong> (034-24024)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-130 from cache -->\n<h5>Reagents for Cryopreservation<\/h5>\n\n<table id=\"tablepress-131\" class=\"tablepress tablepress-id-131\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\"><strong><\/strong><\/th><th class=\"column-2\"><strong>Manufacturer, Catalog Number etc<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">2xFM1<\/td><td class=\"column-2\">Freeze Medium 1, double concentration of DMSO and Methylcellulose, especially useful for serum-free cultured cells<br \/>\n(Ref.: Ohno, T. et al., Cytotechnology 1, 257-260, 1988)\u00a0\u00a0<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">CELLBANKER 1<\/td><td class=\"column-2\">CELLBANKER 1 <strong>Nippon Zenyaku Kogyo Co.,Ltd<\/strong> (CB011)<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">CELLBANKER 2<\/td><td class=\"column-2\">CELLBANKER 2 <strong>Nippon Zenyaku Kogyo Co.,Ltd<\/strong> (CB031)<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">Cell Reservoir One (with DMSO)<\/td><td class=\"column-2\"><strong>Nacalai<\/strong>\u00a0\u00a0(07485-44)<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">STEM-CELLBANKER<\/td><td class=\"column-2\">STEM-CELLBANKER GMP grade,\u00a0<strong>TAKARA<\/strong>\u00a0(CB045)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">CELLBANKER 1plus<\/td><td class=\"column-2\"><strong>Nippon Zenyaku Kogyo Co.,Ltd<\/strong> (CB021)<\/td>\n<\/tr>\n<tr class=\"row-8\">\n\t<td class=\"column-1\">Bambanker<\/td><td class=\"column-2\"><strong>GC Lymphotec<\/strong> (CS-02-002)<\/td>\n<\/tr>\n<tr class=\"row-9\">\n\t<td class=\"column-1\">DMSO<\/td><td class=\"column-2\"><strong>SIGMA<\/strong> (D2650)<\/td>\n<\/tr>\n<tr class=\"row-10\">\n\t<td class=\"column-1\">Glycerol<\/td><td class=\"column-2\"><strong>SIGMA<\/strong> (12-1120-5)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-131 from cache -->\n<h5>Abbreviations<\/h5>\n\n<table id=\"tablepress-132\" class=\"tablepress tablepress-id-132\">\n<thead>\n<tr class=\"row-1\">\n\t<th class=\"column-1\">CS<\/th><th class=\"column-2\">Calf Serum<\/th>\n<\/tr>\n<\/thead>\n<tbody class=\"row-striping\">\n<tr class=\"row-2\">\n\t<td class=\"column-1\">FBS (FCS)<\/td><td class=\"column-2\">Fetal bovine serum (Fetal calf serum)<\/td>\n<\/tr>\n<tr class=\"row-3\">\n\t<td class=\"column-1\">HICS<\/td><td class=\"column-2\">Heat inactivated CS<\/td>\n<\/tr>\n<tr class=\"row-4\">\n\t<td class=\"column-1\">HIFBS<\/td><td class=\"column-2\">Heat inactivated FBS<\/td>\n<\/tr>\n<tr class=\"row-5\">\n\t<td class=\"column-1\">HS<\/td><td class=\"column-2\">Horse Serum<\/td>\n<\/tr>\n<tr class=\"row-6\">\n\t<td class=\"column-1\">NBS (NCS)<\/td><td class=\"column-2\">Newborn bovine Serum (Newborn Calf Serum)<\/td>\n<\/tr>\n<tr class=\"row-7\">\n\t<td class=\"column-1\">WEHI-3-CM<\/td><td class=\"column-2\">WEHI-3 condition medium<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<!-- #tablepress-132 from cache -->\n","protected":false},"excerpt":{"rendered":"<p>During the initial culture period, please begin the culture under the conditions specified by our cell bank. (Please check the datasheet) Products from other manufacturers may be used, provided that they have an equivalent composition. We strongly suggest all users to culture the cells under the culture condition recommended by our cell bank until you make enough stocks. After that, culture the cells with the other protocols of your interest for your research purposes. L-glutamine L-glutamine is an essential component of various basal media. However, due to its instability in aqueous solution, some commercially available media do not contain L-glutamine. In such cases, please supplement the medium with L-glutamine. L-glutamine remains stable when frozen, either completely or partially. However, prolonged storage in a fully thawed state may compromise its stability, particularly at temperatures above 2\u20138 \u00b0C. For long-term storage, L-glutamine should be aliquoted and stored at \u221220 \u00b0C. Heat inactivation of serum In our cell bank, serum is not heat-inactivated unless spesifically requested by the depositor.&#8221; Cttokines The &#8220;r&#8221; shown in the medium compisition on the datesheet stands for recombinant. \u3000\u3000ex\uff1aRPMI1640+10%FBS+10 ng\/mL mouse rIL-3&#8243; Basal Cell Culture Medium Reagents Reagents for Cryopreservation Abbreviations<\/p>\n","protected":false},"author":14,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-8354","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8354","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/14"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=8354"}],"version-history":[{"count":2,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8354\/revisions"}],"predecessor-version":[{"id":11363,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/8354\/revisions\/11363"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=8354"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}