{"id":10329,"date":"2021-09-27T15:43:35","date_gmt":"2021-09-27T06:43:35","guid":{"rendered":"http:\/\/cell.brc.riken.jp\/en\/?page_id=10329"},"modified":"2021-09-27T15:43:35","modified_gmt":"2021-09-27T06:43:35","slug":"cryotubes","status":"publish","type":"page","link":"http:\/\/cell.brc.riken.jp\/en\/cryotubes","title":{"rendered":"Be careful about explosion of cryotubes"},"content":{"rendered":"<p><span style=\"color: #ff0000;\">We are taking appropriate measures to reduce the risk of explosion of cryotubes.<br \/>\nHowever, there remains a possibility that cryotubes explode.<br \/>\nTherefore, please always wear a face guard and protective gloves when handling frozen cryotubes.<\/span><\/p>\n<p><strong>Glass ampules: <\/strong><br \/>\nWe always check cracks or pinholes in all glass ampules before shipping.<br \/>\nHowever, there remains a possibility that cryotubes explode.<br \/>\nOne of the possible causes is a pinhole that we cannot detect by our inspection steps. Liquid nitrogen can come into cryotubes through such a pinhole. When thawing the cells, liquid nitrogen inside cryotubes expands drastically and results in explosion of cryotubes.<br \/>\n<span style=\"color: #ff0000;\">We urge all users to wear a face guard and protective gloves, when taking out cryotubes from a preservation tank. Please check inside the cryotubes whether liquid nitrogen is present.<\/span><\/p>\n<p>An example of explosion:<br \/>\nPut a glass ampule into a 15 mL tube (an outer tube for protection of ampule) on dry ice during shipping procedure.<br \/>\n\u2193<br \/>\nClose the outer lid (styrofoam).<br \/>\n\u2193<br \/>\nExplosion of a glass ampule resulted in a rupture of 15 mL outer tube.<br \/>\n<img decoding=\"async\" src=\"\/en\/wp-content\/uploads\/images\/tube_1.png\"><\/a><\/p>\n<p><strong>Plastic tubes:<\/strong><br \/>\nWe always preserve plastic cryotubes in the vapor-phase of liquid nitrogen tanks, and we always ensure that the caps are tightly fastened before shipping.<br \/>\nWe have received reports from some users regarding explosion of plastic cryotubes.<br \/>\n<span style=\"color: #ff0000;\">Of note, plastic cryotubes must be preserved in the vapor-phase of liquid nitrogen tank.<\/span> Otherwise, liquid nitrogen can come into cryotubes, since screw caps are never air-tight.<br \/>\nIn addition, it is strongly recommended to perform the following step.<br \/>\nBefore thawing the cells, loosen the cap of the plastic cryotubes 1\/4 turn in a safety cabinet to release the pressure inside and then re-tighten the cap.<\/p>\n<p>An example of explosion:<br \/>\nTook out a plastic cryotube out of liquid nitrogen tank for thawing the cells.<br \/>\n\u2193<br \/>\nPlaced the plastic cryotube on dry ice, and then explosion occurred (Photo provided by a user).<br \/>\n<img decoding=\"async\" src=\"\/en\/wp-content\/uploads\/images\/tube_2.png\"><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are taking appropriate measures to reduce the risk of explosion of cryotubes. However, there remains a possibility that cryotubes explode. Therefore, please always wear a face guard and protective gloves when handling frozen cryotubes. Glass ampules: We always check cracks or pinholes in all glass ampules before shipping. However, there remains a possibility that cryotubes explode. One of the possible causes is a pinhole that we cannot detect by our inspection steps. Liquid nitrogen can come into cryotubes through such a pinhole. When thawing the cells, liquid nitrogen inside cryotubes expands drastically and results in explosion of cryotubes. We urge all users to wear a face guard and protective gloves, when taking out cryotubes from a preservation tank. Please check inside the cryotubes whether liquid nitrogen is present. An example of explosion: Put a glass ampule into a 15 mL tube (an outer tube for protection of ampule) on dry ice during shipping procedure. \u2193 Close the outer lid (styrofoam). \u2193 Explosion of a glass ampule resulted in a rupture of 15 mL outer tube. Plastic tubes: We always preserve plastic cryotubes in the vapor-phase of liquid nitrogen tanks, and we always ensure that the caps are tightly fastened [&hellip;]<\/p>\n","protected":false},"author":14,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-10329","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/10329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/14"}],"replies":[{"embeddable":true,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=10329"}],"version-history":[{"count":1,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/10329\/revisions"}],"predecessor-version":[{"id":10331,"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/10329\/revisions\/10331"}],"wp:attachment":[{"href":"http:\/\/cell.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=10329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}