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Technical training
 "Lecture and practice on cryopreservation of human iPS cells (vitrification method)"
Medium List

Medium List

2xFM1Freeze Medium 1, double concentration of DMSO and Methylcellulose, especially useful for serum-free cultured cells (see Ohno, T. et al., Cytotechnology 1, 257-260, 1988)
ASF103ASF103 Ajinomoto, Tokyo, Japan
B27B27 Supplement (50X) GIBCO (17504-044)
Bacto-PeptoneBD (211677)
BES : N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acidSIGMA (B9879)
BIO : (2'Z,3'E)-6-Bromoindirubin-3'-acetoximeBIO-Acetoxime (GSK-3 Inhibitor X ) MERCK (361551)
BM Condimed H1Roche (04155645001)
BMEBasal Medium, Eagle GIBCO (21010-046)
bovine apo-TransferrinTransferrin, Bovine (Apo form), lyophilizedGIBCO (11108-016)
CELLBANKER 1CELLBANKER 1 Nippon Zenyaku Kogyo Co.,Ltd
CELLBANKER 2CELLBANKER 2 Nippon Zenyaku Kogyo Co.,Ltd
CSCalf Serum
CultiCellCultiCell Medium for Embryonic Stem Cells KAC Co. (KSDSRK100) 
Defined Keratinocyte-SFMDefined Keratinocyte-SFM (1X), Liquid, GIBCO (10744-019)
DispaseDispase I Oenon Holdings, Inc.
Dispase IIRoche (4942078001)
DM-160AU(see Katsuta, H. and Takaoka, T. : Methods in Cell Biol. 14, 145-158,1976) KYOKUTO PHARMACEUTICAL INDUSTRIAL CO.,LTD Contact
DM-201KYOKUTO PHARMACEUTICAL INDUSTRIAL CO.,LTD Contact
DMEMD-MEM, liquid, GIBCO (11885-084)
DMEM(high glucose)D-MEM(high glucose), liquid, GIBCO (11995-065)
DMEM(high glucose, without Sodium pyruvate)D-MEM(high glucose), liquid, GIBCO (11965-092)
DMEM(KnockOut)KnockOut D-MEM GIBCO (10829-018)
DMEM/HamF12 SIGMA (without L-Glu ;D6421 , with L-Glu ; D8437)
Earl's BSSEarl's Balanced Salt Solution GIBCO (14155-063)
E-RDFE-RDF medium KYOKUTO PHARMACEUTICAL INDUSTRIAL CO.,LTD (26500) Contact
ESES medium "Nissui" NISSUI PHARMACEUTICAL CO.,LTD (5971)
FBS (FCS)Fetal bovine serum (Fetal calf serum)
G-031101 GlycoTechnica Ltd. (31101) Contact
G418G418 disulfate salt solution (50mg/ml) SIGMA (G8168)
GITserum-Free medium GIT Kohjin-bio Co.,Ltd (637-25715)
GMEMGlasgow MEM GIBCO (11710-035)
Grace's Insect Medium, SupplementedGrace's insect cell culture medium GIBCO (11605-094)
Grace's Insect Medium, UnsupplementedGrace's insect cell culture medium GIBCO (11595-030)
HamF10Ham's F10 medium (Nutrient mixture F-10 Ham) SIGMA (N6908)
HamF12F-12 Nutrient Mixture (HAM) SIGMA (N6658)
HATGIBCO (21060-017)
HEPESGIBCO (15630-080)
HF-RITC80-7Hormon-Free RITC 80-7 medium Reseach Institute for the Functional Peptides Co.,Ltd (IFP-0150) Contact
HICSHeat inactivated CS
HIFBSHeat inactivated FBS
Horse SerumHorse Serum
human InsulinInsulin solution human SIGMA (I9278)
HuMedia-KG2HuMedia-KG2 KURABO (KK-2150S)
IMDMIscove's Modified Dulbecco's Medium SIGMA (I3390)
InsulinInsulin solution from Bovine Pancreas SIGMA (I0516)
IPL-41IPL-41 Insect medium GIBCO (11405081)
iPSellonCardio Incorporated (007001) Contact
Iscove'sIscove's Modified Dulbecco's Medium : IMDM
IWP-2Wnt Inhibitor IWP-2 Stemgent (04-0034) Contact
Keratinocyte-SFMGIBCO (17005-042)
KSRKnockOut Serum Replacement (KSR) GIBCO (10828-028)
L-15Leibovitz's L-15 medium  SIGMA (L1518)
M199GIBCO (11150-059)
M199 (20-25mM HEPES)GIBCO (12340-030)
McCoy's 5A McCoy's 5A medium  GIBCO (16600-082)
MEMSIGMA (M4655)
MEM ALPHAGIBCO (12571-063)
MEM(suspension)S-MEM  GIBCO (11380-037)
MEM Amino Acids SolutionMEM Amino Acids Solution (50x)  GIBCO (11130-051)
MEM Vitamine SolutionMEM Vitamine Solution (100x)  GIBCO (11120-052)
MMMitsuhashi & Maramorosch insect tissue culture medium (see Singh, K.R.P.,Curr. Sci. 36, 506, 1967)
NBS (NCS)Newborn bovine Serum (Newborn Calf Serum)
NCTC109SIGMA (N1140)
NCTC135SIGMA (N5138)
NEAANon-Essential Amino Acids10mM (100x), liquid 100ml  GIBCO (11140-050)
NEAA(ES Cell Qualified)ES Cell Qualified MEM Non-Essential Amino Acids Solution 10mM (100X)  Merck (TMS-001-C)
Nucleosides(ES Cell Qualified)ES Cell Qualified Nucleosides (100X)  Merck (ES-008-D)
Neurobasal Medium (NBM) GIBCO (21103-049)
Pluronic F-68SIGMA (P1300)
PLUSOID-MGlycoTechnica Ltd. Contact
POWEREDBY10GlycoTechnica Ltd. Contact
Primate ES Cell MediumReproCELL (RCHEMD001)
RDF(Glutamine(-))Research Institute for the Functional Peptides Co., Ltd Contact
RPMI 1640RPMI 1640 medium, liquid GIBCO (11875-093)
S-Clone CM-B SEKISUI MEDICAL (521669)
Schneider'sSchneider's drosophila medium  GIBCO (21720-024)
SFM-101SFM101 NISSUI PHARMACEUTICAL CO.,LTD (5963)
STEM-CELLBANKERSTEM-CELLBANKER GMP grade, TAKARA (CB045)
StemFit AK02NAjinomoto, Tokyo, Japan (AK02N)
StemMediumStemMedium Serum Free Media for Mouse ES Cell KAC Co. (KSDSRK100) Contact
Stem Pro-34 SFMGIBCO (10639-011)
Stem Span SFEMStemcell Technologies Inc (09650)
STK2Serum Free Medium for Mesenchymal Stem Cell TWOCELLS COMPANY, LIMITED (KBDSTC102) Contact
T3 3, 3', 5-Triiodo-L-thyronine, SIGMA (T2877)
TC-10Immuno-Biological Laboratories Co., Ltd.
TC-100Baculotechnologies Company Limited
TransferrinTransferrin  SIGMA (T1283)
Ultroser GUltroser G serum substitute, Pall corporation  (15950-017) Contact
WEHI-3-CMWEHI-3 condition medium
William's EWilliam's medium E  GIBCO (12551-032)
Y-27632WAKO (251-00514)