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Quality Control

Quality Control for Distribution

Quality Control at Deposition

 

Quality Control for Distribution

Cell Engineering Division performs quality control (QC) based on resource information provided by the Depositor to ensure the reproducibility of the experimental results.

Please confirm the list and explanations of performed, not-performed and supplemental QC tests.

We would like to ask all the users to confirm the quality and characteristics of our cell resources as soon as possible, before starting your full scale experiments. If there is any question or concern on possible defect, please let us know by email to cellqa.brcriken.jp so that we can take required action. We greatly appreciate all your feedbacks that are necessary for improving the quality of our resources.

●All cell materials excluding human umbilical cord blood cells and human mesenchymal stem cells.
Information of characteristics of cell resources obtained by the depositor/developer are shown in the web catalog.

Performed:

  • Culture each cell line and cryopreserve them in the conditions specified by the depositor/developer and perform the following analyses (#1 and #2) as a random sampling.
    #1. Test the recovery of cells after thawing and confirm the culture to be free of microorganisms such as bacteria and fungi.
    #2. Test the infection of mycoplasma with DNA-staining method.

Not Performed:

  • Analyses other than #1 and #2 above.

Supplemental Tests:

  • When any other analyses for the quality of cells are performed, the results are shown in the web catalog.

●All human cell lines
In relation to human cell lines, there is a standard method to detect misidentified cell lines and thus we routinely perform it for all human cell lines.

Performed:

  • Short Tandem Repeat (STR) polymorphism profiling analysis

 

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For Depositor

Whole activities of Cell Engineering Division, RIKEN BioResource Center depend on the deposit of cell resources and their associated information by scientific community. We greatly appreciate all depositors/developers for the contribution.

In relation to cultured cell resources, genetic mutation, infection by microorganisms and cross-contamination by other cells can occur during culture and should be considered as inevitable events, even though one does best to prevent them to occur. In addition, because of sophistication and complication in experimental systems and intellectual property right issues regarding cell resources, it has become very difficult for any scientist to grasp accurate information. These unintended and undesirable defects or mistakes in the quality of cell resources and associated information should not become causes for a lawsuit by users or third parties against scientists who have generously deposited their resources. With this notion, RIKEN BRC recently revised its Materials Transfer Agreements (MTAs) for Distribution, by adding the indemnification clause in Articles 9 and 10 of the MTA for non-profit academic use or Articles 10 and 11 of the MTA for profit use. As legal obstacles are removed, we would like to ask scientific community for active deposition of cell resources. At the same time, to improve the integrity of science using cell resources, we would like to ask all depositors to provide us the information associated with cell resources as much as possible.

Thank you very much for your cooperation.

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Quality Control at Deposition

Cell Engineering Division performs quality control (QC) based on resource information provided by the Depositor to ensure the reproducibility of the experimental results.

●All cell materials excluding human umbilical cord blood cells and human mesenchymal stem cells.
Information of characteristics of cell resources obtained by the depositor/developer are shown in the web catalog.

Performed:

  • Culture each cell line and cryopreserve them in the conditions specified by the depositor/developer and perform the following analyses.
  • Test the recovery of cells after thawing and confirm the culture to be free of microorganisms such as bacteria and fungi.
  • Test the infection of mycoplasma with DNA-staining method.

Not Performed:

  • Analyses other than those described above.

Supplemental Tests:

  • When any other analyses for the quality of cells are performed, the results are shown in the web catalog.

●All human cell lines
In relation to human cell lines, there is a standard method to detect misidentified cell lines and thus we perform it for all human cell lines.

Performed:

  • Short Tandem Repeat (STR) polymorphism profiling analysis

●A part of mouse cells derived from inbred strains (C57BL/6, BALB/c, C3H, 129, DBA/2)
In relation to a part of mouse cell lines derived from inbred strains (C57BL/6, BALB/c, C3H, 129, DBA/2), we perform the following analysis to confirm the originated strains.

Performed:

  • Simple Sequence Length Polymorphism (SSLP) analysis

●ES/iPS cells
Basically, only the analyses performed for all cell resources are carried out. However, in relation to certain standard cell lines other analyses such as karyotyping, flow cytometry analysis for some markers, teratoma formation and so forth were performed. When such specific analyses are performed, the results are shown in the web catalog for each cell line.

●Human umbilical cord blood cells and human mesenchymal stem cells
We directly distribute the ampoules which were cryopreserved and deposited by the depositors without any culture or analyses in our hand. In relation to the analyses performed in the depositors’ laboratories, please refer to the web catalog for each cell material.

 

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